The Potential of NO3--N Utilization by a Woody Shrub Species Lindera triloba: A Cultivation Test to Estimate the Saturation Point of Soil NO3–-N for Plants

Responses of seedlings of a shrub species, Lindera triloba, grown in perlite culture medium, to nitrate (NO3–-N) supply were investigated to estimate the saturating point of available NO3–-N for plant utilization. NO3–-N concentration and nitrate reductase activity (NRA) in leaves and roots were use...

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Main Authors: Lina Koyama, Naoko Tokuchi, Muneto Hirobe, Keisuke Koba
Format: Article
Language:English
Published: Wiley 2001-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/tsw.2001.378
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author Lina Koyama
Naoko Tokuchi
Muneto Hirobe
Keisuke Koba
author_facet Lina Koyama
Naoko Tokuchi
Muneto Hirobe
Keisuke Koba
author_sort Lina Koyama
collection DOAJ
description Responses of seedlings of a shrub species, Lindera triloba, grown in perlite culture medium, to nitrate (NO3–-N) supply were investigated to estimate the saturating point of available NO3–-N for plant utilization. NO3–-N concentration and nitrate reductase activity (NRA) in leaves and roots were used as indicators of NO3–-N uptake and assimilation by L. triloba. Root NRA increased with NO3–-N supply when concentrations were low and reached a plateau at high NO3–-N concentrations. On the other hand, root NO3–-N concentration increased linearly with NO3–-N supply; therefore, it is suggested that NO3–-N uptake did not limit NO3–-N assimilation by L. triloba. In contrast, leaf NRA and leaf NO3–-N concentration were low and were not influenced by NO3–-N supply. This may be caused by the lack of transport of NO3–-N from roots to leaves. The NO3–-N retained in perlite was compared with NO3–-N pool sizes in soils from a forest where L. triloba occurs naturally to estimate the level of NO3–-N availability to plants in the forest soil. The maximum NO3–-N pool size in the forest soil was comparable to concentrations at which root NRA reached a plateau in perlite cultures. These results indicate that soil NO3–-N availability is below the saturation point for NO3–-N uptake by L. triloba, and it is the limiting factor of NO3–-N utilization by L. trilobaunder field conditions in which this species naturally occurs.
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spelling doaj-art-981e49ff151e4f9eb76a4cbbace445992025-08-20T02:10:09ZengWileyThe Scientific World Journal1537-744X2001-01-01151451910.1100/tsw.2001.378The Potential of NO3--N Utilization by a Woody Shrub Species Lindera triloba: A Cultivation Test to Estimate the Saturation Point of Soil NO3–-N for PlantsLina Koyama0Naoko Tokuchi1Muneto Hirobe2Keisuke Koba3Graduate School of Agriculture, JapanGraduate School of Agriculture, JapanGraduate School of Agriculture, JapanGraduate School of Agriculture, JapanResponses of seedlings of a shrub species, Lindera triloba, grown in perlite culture medium, to nitrate (NO3–-N) supply were investigated to estimate the saturating point of available NO3–-N for plant utilization. NO3–-N concentration and nitrate reductase activity (NRA) in leaves and roots were used as indicators of NO3–-N uptake and assimilation by L. triloba. Root NRA increased with NO3–-N supply when concentrations were low and reached a plateau at high NO3–-N concentrations. On the other hand, root NO3–-N concentration increased linearly with NO3–-N supply; therefore, it is suggested that NO3–-N uptake did not limit NO3–-N assimilation by L. triloba. In contrast, leaf NRA and leaf NO3–-N concentration were low and were not influenced by NO3–-N supply. This may be caused by the lack of transport of NO3–-N from roots to leaves. The NO3–-N retained in perlite was compared with NO3–-N pool sizes in soils from a forest where L. triloba occurs naturally to estimate the level of NO3–-N availability to plants in the forest soil. The maximum NO3–-N pool size in the forest soil was comparable to concentrations at which root NRA reached a plateau in perlite cultures. These results indicate that soil NO3–-N availability is below the saturation point for NO3–-N uptake by L. triloba, and it is the limiting factor of NO3–-N utilization by L. trilobaunder field conditions in which this species naturally occurs.http://dx.doi.org/10.1100/tsw.2001.378
spellingShingle Lina Koyama
Naoko Tokuchi
Muneto Hirobe
Keisuke Koba
The Potential of NO3--N Utilization by a Woody Shrub Species Lindera triloba: A Cultivation Test to Estimate the Saturation Point of Soil NO3–-N for Plants
The Scientific World Journal
title The Potential of NO3--N Utilization by a Woody Shrub Species Lindera triloba: A Cultivation Test to Estimate the Saturation Point of Soil NO3–-N for Plants
title_full The Potential of NO3--N Utilization by a Woody Shrub Species Lindera triloba: A Cultivation Test to Estimate the Saturation Point of Soil NO3–-N for Plants
title_fullStr The Potential of NO3--N Utilization by a Woody Shrub Species Lindera triloba: A Cultivation Test to Estimate the Saturation Point of Soil NO3–-N for Plants
title_full_unstemmed The Potential of NO3--N Utilization by a Woody Shrub Species Lindera triloba: A Cultivation Test to Estimate the Saturation Point of Soil NO3–-N for Plants
title_short The Potential of NO3--N Utilization by a Woody Shrub Species Lindera triloba: A Cultivation Test to Estimate the Saturation Point of Soil NO3–-N for Plants
title_sort potential of no3 n utilization by a woody shrub species lindera triloba a cultivation test to estimate the saturation point of soil no3 n for plants
url http://dx.doi.org/10.1100/tsw.2001.378
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