Effects of a novel bacterial 6-phytase on nutrient digestibility in lactating dairy cows

ABSTRACT: Undigested P and N are excreted and contribute to the environmental effects of dairy farming. Degradation of phytate in the rumen has been shown to be high but variable, leading to incomplete phytate-P (PP) utilization and resultant antinutritional effects of phytate along the gastrointest...

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Main Authors: R. García-González, S. Ped, G. Dusel, C. Koch, T. Christensen, L. Marchal, Y. Dersjant-Li
Format: Article
Language:English
Published: Elsevier 2025-04-01
Series:Journal of Dairy Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S0022030225000712
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author R. García-González
S. Ped
G. Dusel
C. Koch
T. Christensen
L. Marchal
Y. Dersjant-Li
author_facet R. García-González
S. Ped
G. Dusel
C. Koch
T. Christensen
L. Marchal
Y. Dersjant-Li
author_sort R. García-González
collection DOAJ
description ABSTRACT: Undigested P and N are excreted and contribute to the environmental effects of dairy farming. Degradation of phytate in the rumen has been shown to be high but variable, leading to incomplete phytate-P (PP) utilization and resultant antinutritional effects of phytate along the gastrointestinal tract. We investigated the effect of a novel bacterial 6-phytase (PhyG) on the apparent total-tract digestibility (ATTD) and excretion of DM, PP, P, CP, and NDF in lactating dairy cows. Forty-eight lactating Holstein-Friesian cows were assigned to 3 treatments with 16 cows per treatment in a randomized block design. Cows averaged 44.1 kg milk/d and 124 DIM at the experiment start. Diets were fed ad libitum as a TMR that was formulated to meet nutritional requirements, except for P, which was fed at 92% of the recommendation. Treatments comprised a control diet (CON) and the control diet supplemented with 2,000 or 5,000 phytase units/kg DM of PhyG. The experiment lasted for 19 d, including a 14-d diet adaptation period and a 5-d fecal collection period. Dry matter intake did not differ across treatments. The ATTD of PP in the CON group was 88.5%, indicating incomplete phytate degradation. The ATTD of ash, P, and CP increased linearly, whereas the ATTD of PP and NDF increased both linearly and quadratically with increasing PhyG dose. With PhyG at 5,000 phytase units per kg, the ATTD of PP, P, CP, and NDF increased by 9.2%, 13.7%, 4.1%, and 7.5% points above CON, respectively. Fecal excretion of ash, PP, P, and NDF were reduced linearly. Supplementation of PhyG also reduced excretion of PP quadratically, and CP excretion tended to be reduced linearly. Body weight and milk yield did not differ across treatments, but milk protein yield tended to increase linearly with increasing PhyG dose. The addition of PhyG to the diet of lactating dairy cows improved nutrient utilization and decreased nutrient excretion and could therefore contribute to reducing the environmental effects of dairy production.
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publisher Elsevier
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spelling doaj-art-9807a8d282f44495b7caa2da408388fb2025-08-20T02:11:58ZengElsevierJournal of Dairy Science0022-03022025-04-0110843690369910.3168/jds.2024-25663Effects of a novel bacterial 6-phytase on nutrient digestibility in lactating dairy cowsR. García-González0S. Ped1G. Dusel2C. Koch3T. Christensen4L. Marchal5Y. Dersjant-Li6Danisco Animal Nutrition & Health (IFF), 2342BH Oegstgeest, the Netherlands; Corresponding authorUniversity of Applied Sciences Bingen, 55411 Bingen am Rhein, GermanyUniversity of Applied Sciences Bingen, 55411 Bingen am Rhein, GermanyEducational and Research Centre for Animal Husbandry, Hofgut Neumühle, 67728 Münchweiler an der Alsenz, GermanyDanisco Animal Nutrition & Health (IFF), 8220 Brabrand, DenmarkDanisco Animal Nutrition & Health (IFF), 2342BH Oegstgeest, the NetherlandsDanisco Animal Nutrition & Health (IFF), 2342BH Oegstgeest, the NetherlandsABSTRACT: Undigested P and N are excreted and contribute to the environmental effects of dairy farming. Degradation of phytate in the rumen has been shown to be high but variable, leading to incomplete phytate-P (PP) utilization and resultant antinutritional effects of phytate along the gastrointestinal tract. We investigated the effect of a novel bacterial 6-phytase (PhyG) on the apparent total-tract digestibility (ATTD) and excretion of DM, PP, P, CP, and NDF in lactating dairy cows. Forty-eight lactating Holstein-Friesian cows were assigned to 3 treatments with 16 cows per treatment in a randomized block design. Cows averaged 44.1 kg milk/d and 124 DIM at the experiment start. Diets were fed ad libitum as a TMR that was formulated to meet nutritional requirements, except for P, which was fed at 92% of the recommendation. Treatments comprised a control diet (CON) and the control diet supplemented with 2,000 or 5,000 phytase units/kg DM of PhyG. The experiment lasted for 19 d, including a 14-d diet adaptation period and a 5-d fecal collection period. Dry matter intake did not differ across treatments. The ATTD of PP in the CON group was 88.5%, indicating incomplete phytate degradation. The ATTD of ash, P, and CP increased linearly, whereas the ATTD of PP and NDF increased both linearly and quadratically with increasing PhyG dose. With PhyG at 5,000 phytase units per kg, the ATTD of PP, P, CP, and NDF increased by 9.2%, 13.7%, 4.1%, and 7.5% points above CON, respectively. Fecal excretion of ash, PP, P, and NDF were reduced linearly. Supplementation of PhyG also reduced excretion of PP quadratically, and CP excretion tended to be reduced linearly. Body weight and milk yield did not differ across treatments, but milk protein yield tended to increase linearly with increasing PhyG dose. The addition of PhyG to the diet of lactating dairy cows improved nutrient utilization and decreased nutrient excretion and could therefore contribute to reducing the environmental effects of dairy production.http://www.sciencedirect.com/science/article/pii/S0022030225000712digestibilityphosphorusphytasephytate
spellingShingle R. García-González
S. Ped
G. Dusel
C. Koch
T. Christensen
L. Marchal
Y. Dersjant-Li
Effects of a novel bacterial 6-phytase on nutrient digestibility in lactating dairy cows
Journal of Dairy Science
digestibility
phosphorus
phytase
phytate
title Effects of a novel bacterial 6-phytase on nutrient digestibility in lactating dairy cows
title_full Effects of a novel bacterial 6-phytase on nutrient digestibility in lactating dairy cows
title_fullStr Effects of a novel bacterial 6-phytase on nutrient digestibility in lactating dairy cows
title_full_unstemmed Effects of a novel bacterial 6-phytase on nutrient digestibility in lactating dairy cows
title_short Effects of a novel bacterial 6-phytase on nutrient digestibility in lactating dairy cows
title_sort effects of a novel bacterial 6 phytase on nutrient digestibility in lactating dairy cows
topic digestibility
phosphorus
phytase
phytate
url http://www.sciencedirect.com/science/article/pii/S0022030225000712
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