Lysate of Parabacteroides distasonis prevents severe forms of experimental autoimmune encephalomyelitis by modulating the priming of T cell response
The gut microbiota influences the reactivity of the immune system, and Parabacteroides distasonis has emerged as an anti-inflammatory commensal. Here, we investigated whether its lysate could prevent severe forms of neuroinflammation in experimental autoimmune encephalomyelitis (EAE) in mice and how...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1475126/full |
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| author | Zuzana Jiraskova Zakostelska Michal Kraus Stepan Coufal Petra Prochazkova Zaneta Slavickova Tomas Thon Tomas Hrncir Jakub Kreisinger Klara Kostovcikova Pavlina Kleinova Jana Lizrova Preiningerova Miluse Pavelcova Veronika Ticha Ivana Kovarova Eva Kubala Havrdova Helena Tlaskalova-Hogenova Miloslav Kverka |
| author_facet | Zuzana Jiraskova Zakostelska Michal Kraus Stepan Coufal Petra Prochazkova Zaneta Slavickova Tomas Thon Tomas Hrncir Jakub Kreisinger Klara Kostovcikova Pavlina Kleinova Jana Lizrova Preiningerova Miluse Pavelcova Veronika Ticha Ivana Kovarova Eva Kubala Havrdova Helena Tlaskalova-Hogenova Miloslav Kverka |
| author_sort | Zuzana Jiraskova Zakostelska |
| collection | DOAJ |
| description | The gut microbiota influences the reactivity of the immune system, and Parabacteroides distasonis has emerged as an anti-inflammatory commensal. Here, we investigated whether its lysate could prevent severe forms of neuroinflammation in experimental autoimmune encephalomyelitis (EAE) in mice and how this preventive strategy affects the gut microbiota and immune response. Lysate of anaerobically cultured P. distasonis (Pd lysate) was orally administered to C57BL/6 mice in four weekly doses. One week later, EAE was induced and disease severity was assessed three weeks after induction. Fecal microbiota changes in both vehicle- and Pd lysate-treated animals was analyzed by 16S V3–V4 amplicon sequencing and qPCR, antimicrobial peptide expression in the intestinal mucosa was measured by qPCR, and immune cell composition in the mesenteric and inguinal lymph nodes was measured by multicolor flow cytometry. Pd lysate significantly delayed the development of EAE and reduced its severity when administered prior to disease induction. EAE induction was the main factor in altering the gut microbiota, decreasing the abundance of lactobacilli and segmented filamentous bacteria. Pd lysate significantly increased the intestinal abundance of the genera Anaerostipes, Parabacteroides and Prevotella, and altered the expression of antimicrobial peptides in the intestinal mucosa. It significantly increased the frequency of regulatory T cells, induced an anti-inflammatory milieu in mesenteric lymph nodes, and reduced the activation of T cells at the priming site. Pd lysate prevents severe forms of EAE by triggering a T regulatory response and modulating T cell priming to autoantigens. Pd lysate could thus be a future modulator of neuroinflammation that increases the resistance to multiple sclerosis. |
| format | Article |
| id | doaj-art-97ff8d58fcf64dfeb21157fb3d391849 |
| institution | OA Journals |
| issn | 1664-3224 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj-art-97ff8d58fcf64dfeb21157fb3d3918492025-08-20T01:56:44ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-12-011510.3389/fimmu.2024.14751261475126Lysate of Parabacteroides distasonis prevents severe forms of experimental autoimmune encephalomyelitis by modulating the priming of T cell responseZuzana Jiraskova Zakostelska0Michal Kraus1Stepan Coufal2Petra Prochazkova3Zaneta Slavickova4Tomas Thon5Tomas Hrncir6Jakub Kreisinger7Klara Kostovcikova8Pavlina Kleinova9Jana Lizrova Preiningerova10Miluse Pavelcova11Veronika Ticha12Ivana Kovarova13Eva Kubala Havrdova14Helena Tlaskalova-Hogenova15Miloslav Kverka16Laboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences, Novy Hradek, CzechiaLaboratory of Animal Evolutionary Biology, Department of Zoology, Faculty of Science, Charles University, Prague, CzechiaLaboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, CzechiaDepartment of Neurology and Centre of Clinical Neuroscience, First Medical Faculty, Charles University and General Medical Hospital in Prague, Prague, CzechiaDepartment of Neurology and Centre of Clinical Neuroscience, First Medical Faculty, Charles University and General Medical Hospital in Prague, Prague, CzechiaDepartment of Neurology and Centre of Clinical Neuroscience, First Medical Faculty, Charles University and General Medical Hospital in Prague, Prague, CzechiaDepartment of Neurology and Centre of Clinical Neuroscience, First Medical Faculty, Charles University and General Medical Hospital in Prague, Prague, CzechiaDepartment of Neurology and Centre of Clinical Neuroscience, First Medical Faculty, Charles University and General Medical Hospital in Prague, Prague, CzechiaDepartment of Neurology and Centre of Clinical Neuroscience, First Medical Faculty, Charles University and General Medical Hospital in Prague, Prague, CzechiaLaboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Cellular and Molecular Immunology, Institute of Microbiology of the Czech Academy of Sciences, Prague, CzechiaThe gut microbiota influences the reactivity of the immune system, and Parabacteroides distasonis has emerged as an anti-inflammatory commensal. Here, we investigated whether its lysate could prevent severe forms of neuroinflammation in experimental autoimmune encephalomyelitis (EAE) in mice and how this preventive strategy affects the gut microbiota and immune response. Lysate of anaerobically cultured P. distasonis (Pd lysate) was orally administered to C57BL/6 mice in four weekly doses. One week later, EAE was induced and disease severity was assessed three weeks after induction. Fecal microbiota changes in both vehicle- and Pd lysate-treated animals was analyzed by 16S V3–V4 amplicon sequencing and qPCR, antimicrobial peptide expression in the intestinal mucosa was measured by qPCR, and immune cell composition in the mesenteric and inguinal lymph nodes was measured by multicolor flow cytometry. Pd lysate significantly delayed the development of EAE and reduced its severity when administered prior to disease induction. EAE induction was the main factor in altering the gut microbiota, decreasing the abundance of lactobacilli and segmented filamentous bacteria. Pd lysate significantly increased the intestinal abundance of the genera Anaerostipes, Parabacteroides and Prevotella, and altered the expression of antimicrobial peptides in the intestinal mucosa. It significantly increased the frequency of regulatory T cells, induced an anti-inflammatory milieu in mesenteric lymph nodes, and reduced the activation of T cells at the priming site. Pd lysate prevents severe forms of EAE by triggering a T regulatory response and modulating T cell priming to autoantigens. Pd lysate could thus be a future modulator of neuroinflammation that increases the resistance to multiple sclerosis.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1475126/fullmultiple sclerosisexperimental autoimmune encephalomyelitisinflammationParabacteroides distasonismicrobiotaregulatory T cells |
| spellingShingle | Zuzana Jiraskova Zakostelska Michal Kraus Stepan Coufal Petra Prochazkova Zaneta Slavickova Tomas Thon Tomas Hrncir Jakub Kreisinger Klara Kostovcikova Pavlina Kleinova Jana Lizrova Preiningerova Miluse Pavelcova Veronika Ticha Ivana Kovarova Eva Kubala Havrdova Helena Tlaskalova-Hogenova Miloslav Kverka Lysate of Parabacteroides distasonis prevents severe forms of experimental autoimmune encephalomyelitis by modulating the priming of T cell response Frontiers in Immunology multiple sclerosis experimental autoimmune encephalomyelitis inflammation Parabacteroides distasonis microbiota regulatory T cells |
| title | Lysate of Parabacteroides distasonis prevents severe forms of experimental autoimmune encephalomyelitis by modulating the priming of T cell response |
| title_full | Lysate of Parabacteroides distasonis prevents severe forms of experimental autoimmune encephalomyelitis by modulating the priming of T cell response |
| title_fullStr | Lysate of Parabacteroides distasonis prevents severe forms of experimental autoimmune encephalomyelitis by modulating the priming of T cell response |
| title_full_unstemmed | Lysate of Parabacteroides distasonis prevents severe forms of experimental autoimmune encephalomyelitis by modulating the priming of T cell response |
| title_short | Lysate of Parabacteroides distasonis prevents severe forms of experimental autoimmune encephalomyelitis by modulating the priming of T cell response |
| title_sort | lysate of parabacteroides distasonis prevents severe forms of experimental autoimmune encephalomyelitis by modulating the priming of t cell response |
| topic | multiple sclerosis experimental autoimmune encephalomyelitis inflammation Parabacteroides distasonis microbiota regulatory T cells |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1475126/full |
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