Pitfalls and other issues with the MTT assay
While the colorimetric assay commonly referred to as ‘MTT’ does provide an indication of effects of photodamage on mitochondrial enzyme levels, a careful analysis showed that the resulting data were not correlated with a clonogenic assay. While MTT data can be claimed to represent ‘cytotoxicity’, th...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-08-01
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| Series: | Photodiagnosis and Photodynamic Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1572100025002327 |
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| author | David Kessel |
| author_facet | David Kessel |
| author_sort | David Kessel |
| collection | DOAJ |
| description | While the colorimetric assay commonly referred to as ‘MTT’ does provide an indication of effects of photodamage on mitochondrial enzyme levels, a careful analysis showed that the resulting data were not correlated with a clonogenic assay. While MTT data can be claimed to represent ‘cytotoxicity’, this is an undefined term and appears not to be necessarily correlated with the object of anti-tumor therapy: tumor eradication. Use of this assay for assessing effects of treatment on viability are therefore unreliable. |
| format | Article |
| id | doaj-art-97e4b9a7deda4ad7baa01d465ba2ccfb |
| institution | DOAJ |
| issn | 1572-1000 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photodiagnosis and Photodynamic Therapy |
| spelling | doaj-art-97e4b9a7deda4ad7baa01d465ba2ccfb2025-08-20T03:23:22ZengElsevierPhotodiagnosis and Photodynamic Therapy1572-10002025-08-015410470010.1016/j.pdpdt.2025.104700Pitfalls and other issues with the MTT assayDavid Kessel0Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201, USAWhile the colorimetric assay commonly referred to as ‘MTT’ does provide an indication of effects of photodamage on mitochondrial enzyme levels, a careful analysis showed that the resulting data were not correlated with a clonogenic assay. While MTT data can be claimed to represent ‘cytotoxicity’, this is an undefined term and appears not to be necessarily correlated with the object of anti-tumor therapy: tumor eradication. Use of this assay for assessing effects of treatment on viability are therefore unreliable.http://www.sciencedirect.com/science/article/pii/S1572100025002327MTTClonogenicPhotodynamic therapy |
| spellingShingle | David Kessel Pitfalls and other issues with the MTT assay Photodiagnosis and Photodynamic Therapy MTT Clonogenic Photodynamic therapy |
| title | Pitfalls and other issues with the MTT assay |
| title_full | Pitfalls and other issues with the MTT assay |
| title_fullStr | Pitfalls and other issues with the MTT assay |
| title_full_unstemmed | Pitfalls and other issues with the MTT assay |
| title_short | Pitfalls and other issues with the MTT assay |
| title_sort | pitfalls and other issues with the mtt assay |
| topic | MTT Clonogenic Photodynamic therapy |
| url | http://www.sciencedirect.com/science/article/pii/S1572100025002327 |
| work_keys_str_mv | AT davidkessel pitfallsandotherissueswiththemttassay |