The association between parameters of oral mucosal immunity and 25-hydroxyvitamin D in patients with rampant caries
Aim. To determine the saliva level of immunoregulatory proteins in patients with rampant caries and 25-hydroxyvitamin D (25(OH)D) deficiency and evaluate the association of their concentration with 25(OH)D plasma level.Materials and methods. The study was performed in two groups. The experimental gr...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Siberian State Medical University (Tomsk)
2022-01-01
|
| Series: | Бюллетень сибирской медицины |
| Subjects: | |
| Online Access: | https://bulletin.ssmu.ru/jour/article/view/4577 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849773356510871552 |
|---|---|
| author | A. S. Putneva T. M. Karavaeva M. V. Maksimenya P. P. Tereshkov M. N. Mishchenko E. V. Fefelova N. N. Tsybikov A. A. Parshina |
| author_facet | A. S. Putneva T. M. Karavaeva M. V. Maksimenya P. P. Tereshkov M. N. Mishchenko E. V. Fefelova N. N. Tsybikov A. A. Parshina |
| author_sort | A. S. Putneva |
| collection | DOAJ |
| description | Aim. To determine the saliva level of immunoregulatory proteins in patients with rampant caries and 25-hydroxyvitamin D (25(OH)D) deficiency and evaluate the association of their concentration with 25(OH)D plasma level.Materials and methods. The study was performed in two groups. The experimental group included 15 patients aged 20–22 years with rampant caries and the 25(OH)D plasma level of < 20 ng / ml. The control group encompassed 15 healthy age-matched volunteers with the 25(OH)D plasma level of 20–100 ng / ml. The concentrations of B7.2 (CD86), free active TGF-β1, CTLA-4, PD-1, Tim-3, LAG-3, IGFBP-4, and ICAM-1 were assessed using flow cytometry. The levels of LL-37 and secretory immunoglobulin A (sIgA) were measured using ELISA. The Spearman’s rank correlation coefficient was used to reveal a correlation between the indicated proteins and the 25(OH)D plasma level.Results. A decrease in B7.2 (CD86), PD-1, Tim-3, sIgA, and LL-37 and elevation of IGFBP-4 and ICAM-1 saliva levels were detected in patients with rampant caries and 25-hydroxyvitamin D deficiency. A positive Spearman’s rank correlation coefficient was revealed between plasma 25(OH)D and saliva levels of free active TGF-β1, CTLA4, B7.2 (CD86), LL-37, and sIgA. A negative correlation was revealed between 25(OH)Dand ICAM-1.Conclusion. 25(OH)D deficiency in patients with rampant caries is associated with decreased levels of B7.2 (CD86), PD-1, Tim-3, sIgA, and LL-37 and elevated levels of IGFBP-4 and ICAM-1 in the saliva. |
| format | Article |
| id | doaj-art-97d5cba66c5e41f484441bf7a38ee620 |
| institution | DOAJ |
| issn | 1682-0363 1819-3684 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj-art-97d5cba66c5e41f484441bf7a38ee6202025-08-20T03:02:04ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842022-01-01204323810.20538/1682-0363-2021-4-32-382837The association between parameters of oral mucosal immunity and 25-hydroxyvitamin D in patients with rampant cariesA. S. Putneva0T. M. Karavaeva1M. V. Maksimenya2P. P. Tereshkov3M. N. Mishchenko4E. V. Fefelova5N. N. Tsybikov6A. A. Parshina7Chita State Medical Academy (CSMA)Chita State Medical Academy (CSMA)Chita State Medical Academy (CSMA)Chita State Medical Academy (CSMA)Chita State Medical Academy (CSMA)Chita State Medical Academy (CSMA)Chita State Medical Academy (CSMA)Chita State Medical Academy (CSMA)Aim. To determine the saliva level of immunoregulatory proteins in patients with rampant caries and 25-hydroxyvitamin D (25(OH)D) deficiency and evaluate the association of their concentration with 25(OH)D plasma level.Materials and methods. The study was performed in two groups. The experimental group included 15 patients aged 20–22 years with rampant caries and the 25(OH)D plasma level of < 20 ng / ml. The control group encompassed 15 healthy age-matched volunteers with the 25(OH)D plasma level of 20–100 ng / ml. The concentrations of B7.2 (CD86), free active TGF-β1, CTLA-4, PD-1, Tim-3, LAG-3, IGFBP-4, and ICAM-1 were assessed using flow cytometry. The levels of LL-37 and secretory immunoglobulin A (sIgA) were measured using ELISA. The Spearman’s rank correlation coefficient was used to reveal a correlation between the indicated proteins and the 25(OH)D plasma level.Results. A decrease in B7.2 (CD86), PD-1, Tim-3, sIgA, and LL-37 and elevation of IGFBP-4 and ICAM-1 saliva levels were detected in patients with rampant caries and 25-hydroxyvitamin D deficiency. A positive Spearman’s rank correlation coefficient was revealed between plasma 25(OH)D and saliva levels of free active TGF-β1, CTLA4, B7.2 (CD86), LL-37, and sIgA. A negative correlation was revealed between 25(OH)Dand ICAM-1.Conclusion. 25(OH)D deficiency in patients with rampant caries is associated with decreased levels of B7.2 (CD86), PD-1, Tim-3, sIgA, and LL-37 and elevated levels of IGFBP-4 and ICAM-1 in the saliva.https://bulletin.ssmu.ru/jour/article/view/4577rampant caries25-hydroxyvitamin d deficiencymucosal immunity |
| spellingShingle | A. S. Putneva T. M. Karavaeva M. V. Maksimenya P. P. Tereshkov M. N. Mishchenko E. V. Fefelova N. N. Tsybikov A. A. Parshina The association between parameters of oral mucosal immunity and 25-hydroxyvitamin D in patients with rampant caries Бюллетень сибирской медицины rampant caries 25-hydroxyvitamin d deficiency mucosal immunity |
| title | The association between parameters of oral mucosal immunity and 25-hydroxyvitamin D in patients with rampant caries |
| title_full | The association between parameters of oral mucosal immunity and 25-hydroxyvitamin D in patients with rampant caries |
| title_fullStr | The association between parameters of oral mucosal immunity and 25-hydroxyvitamin D in patients with rampant caries |
| title_full_unstemmed | The association between parameters of oral mucosal immunity and 25-hydroxyvitamin D in patients with rampant caries |
| title_short | The association between parameters of oral mucosal immunity and 25-hydroxyvitamin D in patients with rampant caries |
| title_sort | association between parameters of oral mucosal immunity and 25 hydroxyvitamin d in patients with rampant caries |
| topic | rampant caries 25-hydroxyvitamin d deficiency mucosal immunity |
| url | https://bulletin.ssmu.ru/jour/article/view/4577 |
| work_keys_str_mv | AT asputneva theassociationbetweenparametersoforalmucosalimmunityand25hydroxyvitamindinpatientswithrampantcaries AT tmkaravaeva theassociationbetweenparametersoforalmucosalimmunityand25hydroxyvitamindinpatientswithrampantcaries AT mvmaksimenya theassociationbetweenparametersoforalmucosalimmunityand25hydroxyvitamindinpatientswithrampantcaries AT pptereshkov theassociationbetweenparametersoforalmucosalimmunityand25hydroxyvitamindinpatientswithrampantcaries AT mnmishchenko theassociationbetweenparametersoforalmucosalimmunityand25hydroxyvitamindinpatientswithrampantcaries AT evfefelova theassociationbetweenparametersoforalmucosalimmunityand25hydroxyvitamindinpatientswithrampantcaries AT nntsybikov theassociationbetweenparametersoforalmucosalimmunityand25hydroxyvitamindinpatientswithrampantcaries AT aaparshina theassociationbetweenparametersoforalmucosalimmunityand25hydroxyvitamindinpatientswithrampantcaries AT asputneva associationbetweenparametersoforalmucosalimmunityand25hydroxyvitamindinpatientswithrampantcaries AT tmkaravaeva associationbetweenparametersoforalmucosalimmunityand25hydroxyvitamindinpatientswithrampantcaries AT mvmaksimenya associationbetweenparametersoforalmucosalimmunityand25hydroxyvitamindinpatientswithrampantcaries AT pptereshkov associationbetweenparametersoforalmucosalimmunityand25hydroxyvitamindinpatientswithrampantcaries AT mnmishchenko associationbetweenparametersoforalmucosalimmunityand25hydroxyvitamindinpatientswithrampantcaries AT evfefelova associationbetweenparametersoforalmucosalimmunityand25hydroxyvitamindinpatientswithrampantcaries AT nntsybikov associationbetweenparametersoforalmucosalimmunityand25hydroxyvitamindinpatientswithrampantcaries AT aaparshina associationbetweenparametersoforalmucosalimmunityand25hydroxyvitamindinpatientswithrampantcaries |