Influence of Nisin Grafting on the Antibacterial Efficacy of AMP Self-Assembled Monolayers (SAMs)
The use of antimicrobial peptides (AMPs) covalently grafted on surfaces has been recognized in recent years as a promising strategy to fight against biofilm formation. However, after grafting, the understanding of AMP–bacteria interactions is still debated in the literature. In this study, Nisin, a...
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MDPI AG
2024-11-01
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| Series: | Molecules |
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| author | Chloé Richet Adeline Marguier Audrey Bertin Thérèse Leblois Vincent Humblot |
| author_facet | Chloé Richet Adeline Marguier Audrey Bertin Thérèse Leblois Vincent Humblot |
| author_sort | Chloé Richet |
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| description | The use of antimicrobial peptides (AMPs) covalently grafted on surfaces has been recognized in recent years as a promising strategy to fight against biofilm formation. However, after grafting, the understanding of AMP–bacteria interactions is still debated in the literature. In this study, Nisin, a cyclic AMP, was grafted onto gold surfaces via an indirect grafting on acidic thiol self-assembled monolayers using succinimide linkers. The physical and chemical properties of these SAMs were then finely characterized by XPS and FT-IR to confirm the covalent grafting of Nisin. The antiadhesion and bactericidal effects were then studied for <i>Escherichia coli</i> ATCC25922, <i>Staphylococcus aureus</i> ATCC 25923, and <i>Listeria ivanovii</i> Li4(pVS2) by a posteriori analysis of the culture supernatants (i.e., indirect technique) and ex situ by optical microscopy following crystal violet staining (i.e., direct technique). Statistical analysis reveals that the Nisin coating has bactericidal and antiadhesive properties towards Gram-positive bacteria, while no significant results were obtained for Gram-negative bacteria. |
| format | Article |
| id | doaj-art-97c46a2d46d94fc0907e171cd7dc87dd |
| institution | OA Journals |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj-art-97c46a2d46d94fc0907e171cd7dc87dd2025-08-20T02:04:59ZengMDPI AGMolecules1420-30492024-11-012922541710.3390/molecules29225417Influence of Nisin Grafting on the Antibacterial Efficacy of AMP Self-Assembled Monolayers (SAMs)Chloé Richet0Adeline Marguier1Audrey Bertin2Thérèse Leblois3Vincent Humblot4Department of Micro Nano Sciences and Systems (MN2S), Université Franche-Comté, UMR 6174 CNRS, FEMTO-ST Institute, F-25000 Besançon, FranceDepartment of Micro Nano Sciences and Systems (MN2S), Université Franche-Comté, UMR 6174 CNRS, FEMTO-ST Institute, F-25000 Besançon, FranceDepartment of Micro Nano Sciences and Systems (MN2S), Université Franche-Comté, UMR 6174 CNRS, FEMTO-ST Institute, F-25000 Besançon, FranceDepartment of Micro Nano Sciences and Systems (MN2S), Université Franche-Comté, UMR 6174 CNRS, FEMTO-ST Institute, F-25000 Besançon, FranceDepartment of Micro Nano Sciences and Systems (MN2S), Université Franche-Comté, UMR 6174 CNRS, FEMTO-ST Institute, F-25000 Besançon, FranceThe use of antimicrobial peptides (AMPs) covalently grafted on surfaces has been recognized in recent years as a promising strategy to fight against biofilm formation. However, after grafting, the understanding of AMP–bacteria interactions is still debated in the literature. In this study, Nisin, a cyclic AMP, was grafted onto gold surfaces via an indirect grafting on acidic thiol self-assembled monolayers using succinimide linkers. The physical and chemical properties of these SAMs were then finely characterized by XPS and FT-IR to confirm the covalent grafting of Nisin. The antiadhesion and bactericidal effects were then studied for <i>Escherichia coli</i> ATCC25922, <i>Staphylococcus aureus</i> ATCC 25923, and <i>Listeria ivanovii</i> Li4(pVS2) by a posteriori analysis of the culture supernatants (i.e., indirect technique) and ex situ by optical microscopy following crystal violet staining (i.e., direct technique). Statistical analysis reveals that the Nisin coating has bactericidal and antiadhesive properties towards Gram-positive bacteria, while no significant results were obtained for Gram-negative bacteria.https://www.mdpi.com/1420-3049/29/22/5417Nisin coatingantimicrobial peptidebactericidal effectantiadhesive properties |
| spellingShingle | Chloé Richet Adeline Marguier Audrey Bertin Thérèse Leblois Vincent Humblot Influence of Nisin Grafting on the Antibacterial Efficacy of AMP Self-Assembled Monolayers (SAMs) Molecules Nisin coating antimicrobial peptide bactericidal effect antiadhesive properties |
| title | Influence of Nisin Grafting on the Antibacterial Efficacy of AMP Self-Assembled Monolayers (SAMs) |
| title_full | Influence of Nisin Grafting on the Antibacterial Efficacy of AMP Self-Assembled Monolayers (SAMs) |
| title_fullStr | Influence of Nisin Grafting on the Antibacterial Efficacy of AMP Self-Assembled Monolayers (SAMs) |
| title_full_unstemmed | Influence of Nisin Grafting on the Antibacterial Efficacy of AMP Self-Assembled Monolayers (SAMs) |
| title_short | Influence of Nisin Grafting on the Antibacterial Efficacy of AMP Self-Assembled Monolayers (SAMs) |
| title_sort | influence of nisin grafting on the antibacterial efficacy of amp self assembled monolayers sams |
| topic | Nisin coating antimicrobial peptide bactericidal effect antiadhesive properties |
| url | https://www.mdpi.com/1420-3049/29/22/5417 |
| work_keys_str_mv | AT chloerichet influenceofnisingraftingontheantibacterialefficacyofampselfassembledmonolayerssams AT adelinemarguier influenceofnisingraftingontheantibacterialefficacyofampselfassembledmonolayerssams AT audreybertin influenceofnisingraftingontheantibacterialefficacyofampselfassembledmonolayerssams AT thereseleblois influenceofnisingraftingontheantibacterialefficacyofampselfassembledmonolayerssams AT vincenthumblot influenceofnisingraftingontheantibacterialefficacyofampselfassembledmonolayerssams |