Role of the epithelial layer in the generation of superoxide anion by the guinea-pig isolated trachea
The lucigenin-dependent chemiluminescence generation by guinea-pig isolated tracheal two rings preparations was studied. Tracheal preparations stimulated with phorbol myristate acetate (PMA) or opsonized zymosan generated chemiluminescence. The total amount of chemiluminescence generated in 120 min...
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| Format: | Article |
| Language: | English |
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Wiley
1998-01-01
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| Series: | Mediators of Inflammation |
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| Online Access: | http://dx.doi.org/10.1080/09629359891360 |
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| author | G. Sadeghi-Hashjin P. A. J. Henricks G. Folkerts T. Muis J. Garssen F. P. Nijkamp |
| author_facet | G. Sadeghi-Hashjin P. A. J. Henricks G. Folkerts T. Muis J. Garssen F. P. Nijkamp |
| author_sort | G. Sadeghi-Hashjin |
| collection | DOAJ |
| description | The lucigenin-dependent chemiluminescence generation by guinea-pig isolated tracheal two rings preparations was studied. Tracheal preparations stimulated with phorbol myristate acetate (PMA) or opsonized zymosan generated chemiluminescence. The total amount of chemiluminescence generated in 120 min was 754 ± 63 mV × min for PMA and 4832 ±396 mV × min for zymosan. Generation of chemiluminescence was decreased by more than 50% when the tissues were co-incubated with superoxide dismutase (100 U/ml). Also, addition of direct donors of nitric oxide diminished chemiluminescence generation by zymosan-activated tracheal rings significantly by about 50%. However, the presence of the precursor or of inhibitors of nitric oxide synthase did not influence zymosan-induced chemiluminescence. Removal of the epithelial layer from tracheal rings caused an approximately 90% decrease in chemiluminescence response. However, isolated epithelial cell suspensions did not generate chemiluminescence. Histologic examination showed that the number of eosinophils in the tracheal tissue was reduced from 56 ± 7 to 18 ± 8 per mm basal membrane when the epithelial layer was removed. These results indicated that (1) superoxide anion formation can take place in the guinea-pig trachea, (2) eosinophils in the epithelial and submucosal layers of guinea-pig trachea are likely candidates for superoxide generation although other cell types can also be involved, and (3) besides relaxing airway smooth muscle, nitric oxide donors may also affect superoxide in the airways. |
| format | Article |
| id | doaj-art-97acba82fca84e6bba11566522d5e91f |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-97acba82fca84e6bba11566522d5e91f2025-02-03T01:23:41ZengWileyMediators of Inflammation0962-93511466-18611998-01-0171354010.1080/09629359891360Role of the epithelial layer in the generation of superoxide anion by the guinea-pig isolated tracheaG. Sadeghi-Hashjin0P. A. J. Henricks1G. Folkerts2T. Muis3J. Garssen4F. P. Nijkamp5Department of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, PO Box 80.082, Utrecht 3508 TB, The NetherlandsDepartment of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, PO Box 80.082, Utrecht 3508 TB, The NetherlandsDepartment of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, PO Box 80.082, Utrecht 3508 TB, The NetherlandsDepartment of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, PO Box 80.082, Utrecht 3508 TB, The NetherlandsDepartment of Immunobiology and Hematology, National Institute for Public Health and the Environment, PO Box 1, Bilthoven 3720 BA, The NetherlandsDepartment of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, PO Box 80.082, Utrecht 3508 TB, The NetherlandsThe lucigenin-dependent chemiluminescence generation by guinea-pig isolated tracheal two rings preparations was studied. Tracheal preparations stimulated with phorbol myristate acetate (PMA) or opsonized zymosan generated chemiluminescence. The total amount of chemiluminescence generated in 120 min was 754 ± 63 mV × min for PMA and 4832 ±396 mV × min for zymosan. Generation of chemiluminescence was decreased by more than 50% when the tissues were co-incubated with superoxide dismutase (100 U/ml). Also, addition of direct donors of nitric oxide diminished chemiluminescence generation by zymosan-activated tracheal rings significantly by about 50%. However, the presence of the precursor or of inhibitors of nitric oxide synthase did not influence zymosan-induced chemiluminescence. Removal of the epithelial layer from tracheal rings caused an approximately 90% decrease in chemiluminescence response. However, isolated epithelial cell suspensions did not generate chemiluminescence. Histologic examination showed that the number of eosinophils in the tracheal tissue was reduced from 56 ± 7 to 18 ± 8 per mm basal membrane when the epithelial layer was removed. These results indicated that (1) superoxide anion formation can take place in the guinea-pig trachea, (2) eosinophils in the epithelial and submucosal layers of guinea-pig trachea are likely candidates for superoxide generation although other cell types can also be involved, and (3) besides relaxing airway smooth muscle, nitric oxide donors may also affect superoxide in the airways.http://dx.doi.org/10.1080/09629359891360ChemiluminescenceAirwaysEosinophilsNitric oxideOxygen radicals. |
| spellingShingle | G. Sadeghi-Hashjin P. A. J. Henricks G. Folkerts T. Muis J. Garssen F. P. Nijkamp Role of the epithelial layer in the generation of superoxide anion by the guinea-pig isolated trachea Mediators of Inflammation Chemiluminescence Airways Eosinophils Nitric oxide Oxygen radicals. |
| title | Role of the epithelial layer in the generation of superoxide anion by the guinea-pig isolated trachea |
| title_full | Role of the epithelial layer in the generation of superoxide anion by the guinea-pig isolated trachea |
| title_fullStr | Role of the epithelial layer in the generation of superoxide anion by the guinea-pig isolated trachea |
| title_full_unstemmed | Role of the epithelial layer in the generation of superoxide anion by the guinea-pig isolated trachea |
| title_short | Role of the epithelial layer in the generation of superoxide anion by the guinea-pig isolated trachea |
| title_sort | role of the epithelial layer in the generation of superoxide anion by the guinea pig isolated trachea |
| topic | Chemiluminescence Airways Eosinophils Nitric oxide Oxygen radicals. |
| url | http://dx.doi.org/10.1080/09629359891360 |
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