Low-cost modular systems for agarose gel documentation
While conducting recombinant DNA technology procedures, such as DNA purification, agarose gel electrophoresis is often used for identification, characterization and quantification of DNA. The collection of data for experiments involving such techniques frequently involves capturing images using syst...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-11-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/btn-2022-0060 |
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| _version_ | 1850153994124525568 |
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| author | Alfredo G Gonzalez Maribel González-García Rafael Pérez-Ballestero |
| author_facet | Alfredo G Gonzalez Maribel González-García Rafael Pérez-Ballestero |
| author_sort | Alfredo G Gonzalez |
| collection | DOAJ |
| description | While conducting recombinant DNA technology procedures, such as DNA purification, agarose gel electrophoresis is often used for identification, characterization and quantification of DNA. The collection of data for experiments involving such techniques frequently involves capturing images using systems that are expensive and/or proprietary, such that they are not user-serviceable when they malfunction or become antiquated. In response to these limitations, work was done to replace the authors' existing aging Mac OS-based modular system with open-source software and generic hardware. Several versions of a modular imaging system that can be adjusted to fit nearly all use cases are described. The systems developed can accommodate diverse uses from research laboratories to educational environments where commercial systems could be unaffordable. |
| format | Article |
| id | doaj-art-97a99226d3524b0bb22fea0bfaa4d83b |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2022-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-97a99226d3524b0bb22fea0bfaa4d83b2025-08-20T02:25:34ZengTaylor & Francis GroupBioTechniques0736-62051940-98182022-11-0173522723210.2144/btn-2022-0060Low-cost modular systems for agarose gel documentationAlfredo G Gonzalez0Maribel González-García1Rafael Pérez-Ballestero21Department of Chemistry, Texas A&M University-Kingsville, Kingsville, TX 78363, USA1Department of Chemistry, Texas A&M University-Kingsville, Kingsville, TX 78363, USA2Department of Biological & Health Sciences, Texas A&M University-Kingsville, Kingsville, TX 78363, USAWhile conducting recombinant DNA technology procedures, such as DNA purification, agarose gel electrophoresis is often used for identification, characterization and quantification of DNA. The collection of data for experiments involving such techniques frequently involves capturing images using systems that are expensive and/or proprietary, such that they are not user-serviceable when they malfunction or become antiquated. In response to these limitations, work was done to replace the authors' existing aging Mac OS-based modular system with open-source software and generic hardware. Several versions of a modular imaging system that can be adjusted to fit nearly all use cases are described. The systems developed can accommodate diverse uses from research laboratories to educational environments where commercial systems could be unaffordable.https://www.future-science.com/doi/10.2144/btn-2022-0060agarose gelDNAeducationelectrophoresisimagingLinux |
| spellingShingle | Alfredo G Gonzalez Maribel González-García Rafael Pérez-Ballestero Low-cost modular systems for agarose gel documentation BioTechniques agarose gel DNA education electrophoresis imaging Linux |
| title | Low-cost modular systems for agarose gel documentation |
| title_full | Low-cost modular systems for agarose gel documentation |
| title_fullStr | Low-cost modular systems for agarose gel documentation |
| title_full_unstemmed | Low-cost modular systems for agarose gel documentation |
| title_short | Low-cost modular systems for agarose gel documentation |
| title_sort | low cost modular systems for agarose gel documentation |
| topic | agarose gel DNA education electrophoresis imaging Linux |
| url | https://www.future-science.com/doi/10.2144/btn-2022-0060 |
| work_keys_str_mv | AT alfredoggonzalez lowcostmodularsystemsforagarosegeldocumentation AT maribelgonzalezgarcia lowcostmodularsystemsforagarosegeldocumentation AT rafaelperezballestero lowcostmodularsystemsforagarosegeldocumentation |