An Isospora Species (Apicomplexa: Eimeriidae) Identified From a Black‐Faced Cuckoo‐Shrike (Coracina novaehollandiae) (Gmelin, 1789) (Passeriformes: Campephagidae) in Western Australia
ABSTRACT We describe and characterize a new Isospora species from the black‐faced cuckoo‐shrike (Coracina novaehollandiae) in Western Australia, using both morphological and molecular approaches. Microscopic analysis of a fecal sample revealed 20 ellipsoidal oocysts, which are subspheroidal to ovoid...
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Wiley
2025-04-01
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| Series: | Ecology and Evolution |
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| Online Access: | https://doi.org/10.1002/ece3.71298 |
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| author | Yinhua Chen Belinda Brice Bruno P. Berto Qiong Li Rongchang Yang |
| author_facet | Yinhua Chen Belinda Brice Bruno P. Berto Qiong Li Rongchang Yang |
| author_sort | Yinhua Chen |
| collection | DOAJ |
| description | ABSTRACT We describe and characterize a new Isospora species from the black‐faced cuckoo‐shrike (Coracina novaehollandiae) in Western Australia, using both morphological and molecular approaches. Microscopic analysis of a fecal sample revealed 20 ellipsoidal oocysts, which are subspheroidal to ovoid, measuring 40–43 × 39–41 μm (mean 41.4 × 39.6 μm) with a length/width (L/W) ratio of 1.0–1.1 (mean 1.04). The oocyst wall is bi‐layered (~1.5 μm thick), with a smooth outer layer constituting approximately two thirds of the total thickness. A micropyle is present, characterized by a slight invagination of the inner layer (~6.0 μm wide), but no micropyle cap is observed. Both the oocyst residuum and polar granule are absent. Sporocysts (n = 20) are ellipsoidal, measuring 23–24 × 13–14 μm (mean 23.3 × 13.4 μm) with an L/W ratio of 1.7–1.8 (mean 1.74). A flattened to knob‐like Stieda body (~1.5 × 3.0 μm) is present, while sub‐Stieda and para‐Stieda bodies are absent. The sporocyst residuum is composed of granules clustered by a membrane, forming an irregular shape (~12.0 × 8.0 μm). Sporozoites exhibit anterior and posterior refractile bodies and a nucleus. Molecular analyses of the 18S rRNA, 28S rRNA, and COI gene loci demonstrated that this species forms a distinct clade with Isospora serinuse in phylogenetic trees based on all three loci. It shares genetic similarities with I. serinuse of 98.8%, 93.7%, and 98.9% for the 18S rRNA, 28S rRNA, and COI loci, respectively. Phylogenetic analysis confirms that this new species is closely related to I. serinuse. Based on these findings, we propose this isolate as a new species, Isospora coracinae n. sp. This is the first coccidian species identified from the Campephagidae family in Australia. |
| format | Article |
| id | doaj-art-97a8ed9327da446282ce2e8a7ca79486 |
| institution | OA Journals |
| issn | 2045-7758 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wiley |
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| series | Ecology and Evolution |
| spelling | doaj-art-97a8ed9327da446282ce2e8a7ca794862025-08-20T02:35:33ZengWileyEcology and Evolution2045-77582025-04-01154n/an/a10.1002/ece3.71298An Isospora Species (Apicomplexa: Eimeriidae) Identified From a Black‐Faced Cuckoo‐Shrike (Coracina novaehollandiae) (Gmelin, 1789) (Passeriformes: Campephagidae) in Western AustraliaYinhua Chen0Belinda Brice1Bruno P. Berto2Qiong Li3Rongchang Yang4Maotai Institute Renhuai ChinaKanyana Wildlife Rehabilitation Centre Lesmurdie Western Australia AustraliaDepartamento de Biologia Animal, Instituto de Ciências Biológicas e da Saúde Universidade Federal Rural Do Rio de Janeiro Seropédica BrazilMaotai Institute Renhuai ChinaSchool of Agricultural Sciences Murdoch University Murdoch Western Australia AustraliaABSTRACT We describe and characterize a new Isospora species from the black‐faced cuckoo‐shrike (Coracina novaehollandiae) in Western Australia, using both morphological and molecular approaches. Microscopic analysis of a fecal sample revealed 20 ellipsoidal oocysts, which are subspheroidal to ovoid, measuring 40–43 × 39–41 μm (mean 41.4 × 39.6 μm) with a length/width (L/W) ratio of 1.0–1.1 (mean 1.04). The oocyst wall is bi‐layered (~1.5 μm thick), with a smooth outer layer constituting approximately two thirds of the total thickness. A micropyle is present, characterized by a slight invagination of the inner layer (~6.0 μm wide), but no micropyle cap is observed. Both the oocyst residuum and polar granule are absent. Sporocysts (n = 20) are ellipsoidal, measuring 23–24 × 13–14 μm (mean 23.3 × 13.4 μm) with an L/W ratio of 1.7–1.8 (mean 1.74). A flattened to knob‐like Stieda body (~1.5 × 3.0 μm) is present, while sub‐Stieda and para‐Stieda bodies are absent. The sporocyst residuum is composed of granules clustered by a membrane, forming an irregular shape (~12.0 × 8.0 μm). Sporozoites exhibit anterior and posterior refractile bodies and a nucleus. Molecular analyses of the 18S rRNA, 28S rRNA, and COI gene loci demonstrated that this species forms a distinct clade with Isospora serinuse in phylogenetic trees based on all three loci. It shares genetic similarities with I. serinuse of 98.8%, 93.7%, and 98.9% for the 18S rRNA, 28S rRNA, and COI loci, respectively. Phylogenetic analysis confirms that this new species is closely related to I. serinuse. Based on these findings, we propose this isolate as a new species, Isospora coracinae n. sp. This is the first coccidian species identified from the Campephagidae family in Australia.https://doi.org/10.1002/ece3.7129818S rRNA28S rRNAblack‐faced cuckoo‐shrikeCoccidiaCOI geneIsospora |
| spellingShingle | Yinhua Chen Belinda Brice Bruno P. Berto Qiong Li Rongchang Yang An Isospora Species (Apicomplexa: Eimeriidae) Identified From a Black‐Faced Cuckoo‐Shrike (Coracina novaehollandiae) (Gmelin, 1789) (Passeriformes: Campephagidae) in Western Australia Ecology and Evolution 18S rRNA 28S rRNA black‐faced cuckoo‐shrike Coccidia COI gene Isospora |
| title | An Isospora Species (Apicomplexa: Eimeriidae) Identified From a Black‐Faced Cuckoo‐Shrike (Coracina novaehollandiae) (Gmelin, 1789) (Passeriformes: Campephagidae) in Western Australia |
| title_full | An Isospora Species (Apicomplexa: Eimeriidae) Identified From a Black‐Faced Cuckoo‐Shrike (Coracina novaehollandiae) (Gmelin, 1789) (Passeriformes: Campephagidae) in Western Australia |
| title_fullStr | An Isospora Species (Apicomplexa: Eimeriidae) Identified From a Black‐Faced Cuckoo‐Shrike (Coracina novaehollandiae) (Gmelin, 1789) (Passeriformes: Campephagidae) in Western Australia |
| title_full_unstemmed | An Isospora Species (Apicomplexa: Eimeriidae) Identified From a Black‐Faced Cuckoo‐Shrike (Coracina novaehollandiae) (Gmelin, 1789) (Passeriformes: Campephagidae) in Western Australia |
| title_short | An Isospora Species (Apicomplexa: Eimeriidae) Identified From a Black‐Faced Cuckoo‐Shrike (Coracina novaehollandiae) (Gmelin, 1789) (Passeriformes: Campephagidae) in Western Australia |
| title_sort | isospora species apicomplexa eimeriidae identified from a black faced cuckoo shrike coracina novaehollandiae gmelin 1789 passeriformes campephagidae in western australia |
| topic | 18S rRNA 28S rRNA black‐faced cuckoo‐shrike Coccidia COI gene Isospora |
| url | https://doi.org/10.1002/ece3.71298 |
| work_keys_str_mv | AT yinhuachen anisosporaspeciesapicomplexaeimeriidaeidentifiedfromablackfacedcuckooshrikecoracinanovaehollandiaegmelin1789passeriformescampephagidaeinwesternaustralia AT belindabrice anisosporaspeciesapicomplexaeimeriidaeidentifiedfromablackfacedcuckooshrikecoracinanovaehollandiaegmelin1789passeriformescampephagidaeinwesternaustralia AT brunopberto anisosporaspeciesapicomplexaeimeriidaeidentifiedfromablackfacedcuckooshrikecoracinanovaehollandiaegmelin1789passeriformescampephagidaeinwesternaustralia AT qiongli anisosporaspeciesapicomplexaeimeriidaeidentifiedfromablackfacedcuckooshrikecoracinanovaehollandiaegmelin1789passeriformescampephagidaeinwesternaustralia AT rongchangyang anisosporaspeciesapicomplexaeimeriidaeidentifiedfromablackfacedcuckooshrikecoracinanovaehollandiaegmelin1789passeriformescampephagidaeinwesternaustralia AT yinhuachen isosporaspeciesapicomplexaeimeriidaeidentifiedfromablackfacedcuckooshrikecoracinanovaehollandiaegmelin1789passeriformescampephagidaeinwesternaustralia AT belindabrice isosporaspeciesapicomplexaeimeriidaeidentifiedfromablackfacedcuckooshrikecoracinanovaehollandiaegmelin1789passeriformescampephagidaeinwesternaustralia AT brunopberto isosporaspeciesapicomplexaeimeriidaeidentifiedfromablackfacedcuckooshrikecoracinanovaehollandiaegmelin1789passeriformescampephagidaeinwesternaustralia AT qiongli isosporaspeciesapicomplexaeimeriidaeidentifiedfromablackfacedcuckooshrikecoracinanovaehollandiaegmelin1789passeriformescampephagidaeinwesternaustralia AT rongchangyang isosporaspeciesapicomplexaeimeriidaeidentifiedfromablackfacedcuckooshrikecoracinanovaehollandiaegmelin1789passeriformescampephagidaeinwesternaustralia |