Hydrogen-Releasing Micromaterial Dressings: Promoting Wound Healing by Modulating Extracellular Matrix Accumulation Through Wnt/β-Catenin and TGF-β/Smad Pathways
<b>Background</b>: Wound healing is a complex and intricate biological process that involves multiple systems within the body and initiates a series of highly coordinated responses to repair damage and restore integrity and functionality. We previously identified that breathing hydrogen...
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2025-02-01
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| author | Pengxiang Zhao Yufei Li Boyuan Guo Ziyi Liu Xujuan Zhang Mengyu Liu Xuemei Ma |
| author_facet | Pengxiang Zhao Yufei Li Boyuan Guo Ziyi Liu Xujuan Zhang Mengyu Liu Xuemei Ma |
| author_sort | Pengxiang Zhao |
| collection | DOAJ |
| description | <b>Background</b>: Wound healing is a complex and intricate biological process that involves multiple systems within the body and initiates a series of highly coordinated responses to repair damage and restore integrity and functionality. We previously identified that breathing hydrogen can significantly inhibit early inflammation, activate autologous stem cells, and promote the accumulation of extracellular matrix (ECM). However, the broader functions and downstream targets of hydrogen-induced ECM accumulation and tissue remodeling are unknown in the wound-healing process. <b>Methods</b>: Consequently, this thesis developed a hydrogen sustained-release dressing based on a micro storage material and reveals the mechanism of hydrogen in treating wound healing. Upon encapsulating the hydrogen storage materials, magnesium (Mg), and ammonia borane (AB), we found that SiO<sub>2</sub>@Mg exhibits superior sustained-release performance, while SiO<sub>2</sub>@AB demonstrates a higher hydrogen storage capacity. We used a <i>C57/BL6</i> mouse full-thickness skin defect wound model to analyze and compare different hydrogen dressings. <b>Results</b>: It was identified that hydrogen dressings can significantly improve the healing rate of wounds by promoting epithelialization, angiogenesis, and collagen accumulation in wound tissue, and that the effect of slow-release dressings is better than of non-slow-release dressings. We also found that hydrogen dressing can promote transcriptome-level expression related to cell proliferation and differentiation and ECM accumulation, mainly through the <i>Wnt1/β-catenin</i> pathway and <i>TGF-β1/Smad2</i> pathway. <b>Conclusions</b>: Overall, these results provide a novel insight into the field of hydrogen treatment and wound healing. |
| format | Article |
| id | doaj-art-97a6690d528646dda097e6c4dbed5f0a |
| institution | Kabale University |
| issn | 1999-4923 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj-art-97a6690d528646dda097e6c4dbed5f0a2025-08-20T03:43:29ZengMDPI AGPharmaceutics1999-49232025-02-0117327910.3390/pharmaceutics17030279Hydrogen-Releasing Micromaterial Dressings: Promoting Wound Healing by Modulating Extracellular Matrix Accumulation Through Wnt/β-Catenin and TGF-β/Smad PathwaysPengxiang Zhao0Yufei Li1Boyuan Guo2Ziyi Liu3Xujuan Zhang4Mengyu Liu5Xuemei Ma6College of Chemistry and Life Sciences, Beijing University of Technology, Beijing 100124, ChinaCollege of Chemistry and Life Sciences, Beijing University of Technology, Beijing 100124, ChinaCollege of Chemistry and Life Sciences, Beijing University of Technology, Beijing 100124, ChinaCollege of Chemistry and Life Sciences, Beijing University of Technology, Beijing 100124, ChinaCollege of Chemistry and Life Sciences, Beijing University of Technology, Beijing 100124, ChinaCollege of Chemistry and Life Sciences, Beijing University of Technology, Beijing 100124, ChinaCollege of Chemistry and Life Sciences, Beijing University of Technology, Beijing 100124, China<b>Background</b>: Wound healing is a complex and intricate biological process that involves multiple systems within the body and initiates a series of highly coordinated responses to repair damage and restore integrity and functionality. We previously identified that breathing hydrogen can significantly inhibit early inflammation, activate autologous stem cells, and promote the accumulation of extracellular matrix (ECM). However, the broader functions and downstream targets of hydrogen-induced ECM accumulation and tissue remodeling are unknown in the wound-healing process. <b>Methods</b>: Consequently, this thesis developed a hydrogen sustained-release dressing based on a micro storage material and reveals the mechanism of hydrogen in treating wound healing. Upon encapsulating the hydrogen storage materials, magnesium (Mg), and ammonia borane (AB), we found that SiO<sub>2</sub>@Mg exhibits superior sustained-release performance, while SiO<sub>2</sub>@AB demonstrates a higher hydrogen storage capacity. We used a <i>C57/BL6</i> mouse full-thickness skin defect wound model to analyze and compare different hydrogen dressings. <b>Results</b>: It was identified that hydrogen dressings can significantly improve the healing rate of wounds by promoting epithelialization, angiogenesis, and collagen accumulation in wound tissue, and that the effect of slow-release dressings is better than of non-slow-release dressings. We also found that hydrogen dressing can promote transcriptome-level expression related to cell proliferation and differentiation and ECM accumulation, mainly through the <i>Wnt1/β-catenin</i> pathway and <i>TGF-β1/Smad2</i> pathway. <b>Conclusions</b>: Overall, these results provide a novel insight into the field of hydrogen treatment and wound healing.https://www.mdpi.com/1999-4923/17/3/279wound healinghydrogen-releasing micromaterialextracellular matrix depositionangiogenesismolecular mechanism |
| spellingShingle | Pengxiang Zhao Yufei Li Boyuan Guo Ziyi Liu Xujuan Zhang Mengyu Liu Xuemei Ma Hydrogen-Releasing Micromaterial Dressings: Promoting Wound Healing by Modulating Extracellular Matrix Accumulation Through Wnt/β-Catenin and TGF-β/Smad Pathways Pharmaceutics wound healing hydrogen-releasing micromaterial extracellular matrix deposition angiogenesis molecular mechanism |
| title | Hydrogen-Releasing Micromaterial Dressings: Promoting Wound Healing by Modulating Extracellular Matrix Accumulation Through Wnt/β-Catenin and TGF-β/Smad Pathways |
| title_full | Hydrogen-Releasing Micromaterial Dressings: Promoting Wound Healing by Modulating Extracellular Matrix Accumulation Through Wnt/β-Catenin and TGF-β/Smad Pathways |
| title_fullStr | Hydrogen-Releasing Micromaterial Dressings: Promoting Wound Healing by Modulating Extracellular Matrix Accumulation Through Wnt/β-Catenin and TGF-β/Smad Pathways |
| title_full_unstemmed | Hydrogen-Releasing Micromaterial Dressings: Promoting Wound Healing by Modulating Extracellular Matrix Accumulation Through Wnt/β-Catenin and TGF-β/Smad Pathways |
| title_short | Hydrogen-Releasing Micromaterial Dressings: Promoting Wound Healing by Modulating Extracellular Matrix Accumulation Through Wnt/β-Catenin and TGF-β/Smad Pathways |
| title_sort | hydrogen releasing micromaterial dressings promoting wound healing by modulating extracellular matrix accumulation through wnt β catenin and tgf β smad pathways |
| topic | wound healing hydrogen-releasing micromaterial extracellular matrix deposition angiogenesis molecular mechanism |
| url | https://www.mdpi.com/1999-4923/17/3/279 |
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