Cerebrospinal fluid-contacting neurons are sensory neurons with uniform morphological and region-specific electrophysiological properties in the mouse spinal cord
Abstract Cerebrospinal Fluid-contacting neurons (CSF-cNs) are GABAergic bipolar neurons found, in contact with the cerebrospinal fluid, along the vertebrate medullo-spinal central canal. They express Polycystin Kidney Disease 2-Like 1 channels (PKD2L1), members of the Transient Receptor Potential su...
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Nature Portfolio
2025-08-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-08559-x |
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| author | Elysa Crozat Edith Blasco Jorge Ramirez-Franco Priscille Riondel Nina Jurčić Riad Seddik Caroline Michelle Jérôme Trouslard Nicolas Wanaverbecq |
| author_facet | Elysa Crozat Edith Blasco Jorge Ramirez-Franco Priscille Riondel Nina Jurčić Riad Seddik Caroline Michelle Jérôme Trouslard Nicolas Wanaverbecq |
| author_sort | Elysa Crozat |
| collection | DOAJ |
| description | Abstract Cerebrospinal Fluid-contacting neurons (CSF-cNs) are GABAergic bipolar neurons found, in contact with the cerebrospinal fluid, along the vertebrate medullo-spinal central canal. They express Polycystin Kidney Disease 2-Like 1 channels (PKD2L1), members of the Transient Receptor Potential superfamily, and were shown to modulate motor activity and therefore suggested to act as a novel sensory system. However, in mice, they remain largely uncharacterized and it is crucial to comprehensively characterize their morphological and electrophysiological properties to determine whether they form a homogenous neuronal population and understand their role in the CNS. We show that CSF-cNs are distributed throughout the spinal cord with a uniform morphology and a primarily ventral localization. They exhibit region-specific properties, expression of voltage-dependent and ligand-gated conductances and detect variation in extracellular pH through activation of PKD2L1 and Acid-sensing Ion Channels. They possess GABAB and muscarinic receptors, not glutamatergic metabotropic ones, to modulate Ca2+ channels. CSF-cNs represent unique sensory neurons with a uniform morphology and electrophysiological properties that appear specific to the spinal cord segment inserted in. The future challenges in the field, will be to elucidate the physiological stimuli activating CSF-cNs and the neuronal network they are integrated in to modulate body function through specific local spinal network. |
| format | Article |
| id | doaj-art-97a386aa69cb44be8384eb2f487cc291 |
| institution | Kabale University |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
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| series | Communications Biology |
| spelling | doaj-art-97a386aa69cb44be8384eb2f487cc2912025-08-20T03:44:06ZengNature PortfolioCommunications Biology2399-36422025-08-018112410.1038/s42003-025-08559-xCerebrospinal fluid-contacting neurons are sensory neurons with uniform morphological and region-specific electrophysiological properties in the mouse spinal cordElysa Crozat0Edith Blasco1Jorge Ramirez-Franco2Priscille Riondel3Nina Jurčić4Riad Seddik5Caroline Michelle6Jérôme Trouslard7Nicolas Wanaverbecq8Institut de Neurosciences de la Timone, Aix Marseille Université (AMU) & CNRSInstitut de Neurosciences de la Timone, Aix Marseille Université (AMU) & CNRSInstitut de Neurosciences de la Timone, Aix Marseille Université (AMU) & CNRSInstitut de Neurosciences de la Timone, Aix Marseille Université (AMU) & CNRSInstitut de Neurosciences de la Timone, Aix Marseille Université (AMU) & CNRSInstitut de Neurosciences de la Timone, Aix Marseille Université (AMU) & CNRSInstitut de Neurosciences de la Timone, Aix Marseille Université (AMU) & CNRSInstitut de Neurosciences de la Timone, Aix Marseille Université (AMU) & CNRSInstitut de Neurosciences de la Timone, Aix Marseille Université (AMU) & CNRSAbstract Cerebrospinal Fluid-contacting neurons (CSF-cNs) are GABAergic bipolar neurons found, in contact with the cerebrospinal fluid, along the vertebrate medullo-spinal central canal. They express Polycystin Kidney Disease 2-Like 1 channels (PKD2L1), members of the Transient Receptor Potential superfamily, and were shown to modulate motor activity and therefore suggested to act as a novel sensory system. However, in mice, they remain largely uncharacterized and it is crucial to comprehensively characterize their morphological and electrophysiological properties to determine whether they form a homogenous neuronal population and understand their role in the CNS. We show that CSF-cNs are distributed throughout the spinal cord with a uniform morphology and a primarily ventral localization. They exhibit region-specific properties, expression of voltage-dependent and ligand-gated conductances and detect variation in extracellular pH through activation of PKD2L1 and Acid-sensing Ion Channels. They possess GABAB and muscarinic receptors, not glutamatergic metabotropic ones, to modulate Ca2+ channels. CSF-cNs represent unique sensory neurons with a uniform morphology and electrophysiological properties that appear specific to the spinal cord segment inserted in. The future challenges in the field, will be to elucidate the physiological stimuli activating CSF-cNs and the neuronal network they are integrated in to modulate body function through specific local spinal network.https://doi.org/10.1038/s42003-025-08559-x |
| spellingShingle | Elysa Crozat Edith Blasco Jorge Ramirez-Franco Priscille Riondel Nina Jurčić Riad Seddik Caroline Michelle Jérôme Trouslard Nicolas Wanaverbecq Cerebrospinal fluid-contacting neurons are sensory neurons with uniform morphological and region-specific electrophysiological properties in the mouse spinal cord Communications Biology |
| title | Cerebrospinal fluid-contacting neurons are sensory neurons with uniform morphological and region-specific electrophysiological properties in the mouse spinal cord |
| title_full | Cerebrospinal fluid-contacting neurons are sensory neurons with uniform morphological and region-specific electrophysiological properties in the mouse spinal cord |
| title_fullStr | Cerebrospinal fluid-contacting neurons are sensory neurons with uniform morphological and region-specific electrophysiological properties in the mouse spinal cord |
| title_full_unstemmed | Cerebrospinal fluid-contacting neurons are sensory neurons with uniform morphological and region-specific electrophysiological properties in the mouse spinal cord |
| title_short | Cerebrospinal fluid-contacting neurons are sensory neurons with uniform morphological and region-specific electrophysiological properties in the mouse spinal cord |
| title_sort | cerebrospinal fluid contacting neurons are sensory neurons with uniform morphological and region specific electrophysiological properties in the mouse spinal cord |
| url | https://doi.org/10.1038/s42003-025-08559-x |
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