Effect of Carbon Sources on Glutamate Production from Corynebacterium glutamicum 2262

A comparative study between natural and synthetic environments is carried out by realizing six fermentation experiments in batch culture. The objective of this study is to consider the effect of carbon sources on growth and the production of glutamic acid by Corynebacterium glutamicum 2262. The diff...

Full description

Saved in:
Bibliographic Details
Main Authors: Abdenacer Mouffok, Djedjiga Bellouche, Nabil Nancib, Joseph Boudrant
Format: Article
Language:English
Published: Hasan Eleroğlu 2022-12-01
Series:Turkish Journal of Agriculture: Food Science and Technology
Subjects:
Online Access:http://www.agrifoodscience.com/index.php/TURJAF/article/view/5091
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850248926750310400
author Abdenacer Mouffok
Djedjiga Bellouche
Nabil Nancib
Joseph Boudrant
author_facet Abdenacer Mouffok
Djedjiga Bellouche
Nabil Nancib
Joseph Boudrant
author_sort Abdenacer Mouffok
collection DOAJ
description A comparative study between natural and synthetic environments is carried out by realizing six fermentation experiments in batch culture. The objective of this study is to consider the effect of carbon sources on growth and the production of glutamic acid by Corynebacterium glutamicum 2262. The difference between the culture media lies in the carbon source. Two natural environments of date juice, one of which is treated with invertase to hydrolyse sucrose with a concentration of total sugars of 88 g/L. Four synthetic media with a concentration of 34 g/L sugars: medium containing mixed sugars (glucose + fructose + sucrose) with rates proportional to that of date juice, the other three media are composed of a single sugar (either glucose, fructose, or sucrose). The results showed that Corynebacterium glutamicum is able to use the three sugars whether they are single or mixed, although the best results of glutamate production (8.41 g/L) are obtained on the mixture of three sugars, which explains the interest and valorisation of date waste. On the other hand, the date juice-based media are shown to have a glutamate concentration of 7.98 g/L during the hydrolysis of sucrose of date juice.
format Article
id doaj-art-97a383f9b7ea4bef9256c62a3960f625
institution OA Journals
issn 2148-127X
language English
publishDate 2022-12-01
publisher Hasan Eleroğlu
record_format Article
series Turkish Journal of Agriculture: Food Science and Technology
spelling doaj-art-97a383f9b7ea4bef9256c62a3960f6252025-08-20T01:58:35ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2022-12-0110122300230610.24925/turjaf.v10i12.2300-2306.50912673Effect of Carbon Sources on Glutamate Production from Corynebacterium glutamicum 2262Abdenacer Mouffok0Djedjiga Bellouche1Nabil Nancib2Joseph Boudrant3Laboratory of Applied Microbiology, Departement of Microbiologie, Faculty of Natural Sciences, University of Ferhat Abbas Setif 1, 19000Laboratory of Applied Microbiology, Ferhat Abbas University, Setif-1Laboratory of Applied Microbiology, Ferhat Abbas University, Setif-1Laboratory Reactions and Chemical Engineering (LRGP), University of Nancy, Vandoeuvre-les Nancy 54505.A comparative study between natural and synthetic environments is carried out by realizing six fermentation experiments in batch culture. The objective of this study is to consider the effect of carbon sources on growth and the production of glutamic acid by Corynebacterium glutamicum 2262. The difference between the culture media lies in the carbon source. Two natural environments of date juice, one of which is treated with invertase to hydrolyse sucrose with a concentration of total sugars of 88 g/L. Four synthetic media with a concentration of 34 g/L sugars: medium containing mixed sugars (glucose + fructose + sucrose) with rates proportional to that of date juice, the other three media are composed of a single sugar (either glucose, fructose, or sucrose). The results showed that Corynebacterium glutamicum is able to use the three sugars whether they are single or mixed, although the best results of glutamate production (8.41 g/L) are obtained on the mixture of three sugars, which explains the interest and valorisation of date waste. On the other hand, the date juice-based media are shown to have a glutamate concentration of 7.98 g/L during the hydrolysis of sucrose of date juice.http://www.agrifoodscience.com/index.php/TURJAF/article/view/5091date syrupglutamatecorynebacterium glutamicum 2262batch culturecarbon sources
spellingShingle Abdenacer Mouffok
Djedjiga Bellouche
Nabil Nancib
Joseph Boudrant
Effect of Carbon Sources on Glutamate Production from Corynebacterium glutamicum 2262
Turkish Journal of Agriculture: Food Science and Technology
date syrup
glutamate
corynebacterium glutamicum 2262
batch culture
carbon sources
title Effect of Carbon Sources on Glutamate Production from Corynebacterium glutamicum 2262
title_full Effect of Carbon Sources on Glutamate Production from Corynebacterium glutamicum 2262
title_fullStr Effect of Carbon Sources on Glutamate Production from Corynebacterium glutamicum 2262
title_full_unstemmed Effect of Carbon Sources on Glutamate Production from Corynebacterium glutamicum 2262
title_short Effect of Carbon Sources on Glutamate Production from Corynebacterium glutamicum 2262
title_sort effect of carbon sources on glutamate production from corynebacterium glutamicum 2262
topic date syrup
glutamate
corynebacterium glutamicum 2262
batch culture
carbon sources
url http://www.agrifoodscience.com/index.php/TURJAF/article/view/5091
work_keys_str_mv AT abdenacermouffok effectofcarbonsourcesonglutamateproductionfromcorynebacteriumglutamicum2262
AT djedjigabellouche effectofcarbonsourcesonglutamateproductionfromcorynebacteriumglutamicum2262
AT nabilnancib effectofcarbonsourcesonglutamateproductionfromcorynebacteriumglutamicum2262
AT josephboudrant effectofcarbonsourcesonglutamateproductionfromcorynebacteriumglutamicum2262