Mesenchymal Stem Cell-Derived Exosomes Enhance FGF-1 and SDF-1 Expression in Rats with Second Degree Burns
Background: Second-degree burns cause extensive damage to the skin and pose significant health challenges, with current treatments facing limitations such as donor skin shortages and complications. Fibroblast growth factor 1 (FGF-1) and stromal-derived growth factor 1 (SDF-1) are critical for tissue...
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Cell and BioPharmaceutical Institute
2025-07-01
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| Series: | MCBS (Molecular and Cellular Biomedical Sciences) |
| Online Access: | https://cellbiopharm.com/ojs/index.php/MCBS/article/view/635 |
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| author | Nova Putri Hariani Agung Putra Prasetyowati Subchan Eko Setiawan |
| author_facet | Nova Putri Hariani Agung Putra Prasetyowati Subchan Eko Setiawan |
| author_sort | Nova Putri Hariani |
| collection | DOAJ |
| description | Background: Second-degree burns cause extensive damage to the skin and pose significant health challenges, with current treatments facing limitations such as donor skin shortages and complications. Fibroblast growth factor 1 (FGF-1) and stromal-derived growth factor 1 (SDF-1) are critical for tissue repair. Emerging evidence suggests that mesenchymal stem cell-derived exosomes (E-MSCs) are a promising cell-free therapeutic option for enhancing wound healing through the modulation of FGF-1 and SDF-1. This study investigated the effect of E-MSCs on the expression of FGF-1 and SDF-1 genes in rats with second-degree burns.
Materials and methods: This experimental study used a second-degree burn model in Wistar rats, treated with subcutaneous injections of E-MSCs at doses of 100 µL and 200 µL. Gene expression of FGF-1 and SDF-1 was quantified using qRT-PCR. Histological validation confirmed burn severity, and flow cytometry was used to characterize E-MSCs and exosomes.
Results: An increase in FGF-1 and SDF-1 expression was observed in exosome-treated groups compared to the NaCL-treated group. The 200 µL E-MSCs-treated group showed the most significant enhancement in both growth factors, with statistically significant differences (p<0.05). These findings underline the efficacy of E-MSCs in modulating critical genes involved in wound healing.
Conclusion: E-MSCs significantly upregulate FGF-1 and SDF-1 expression, promoting tissue repair and regeneration in second-degree burn models. This study highlights the potential of E-MSCs as a non-invasive therapeutic approach.
Keywords: exosomes, FGF-1, mesenchymal stem cells, SDF-1 |
| format | Article |
| id | doaj-art-9799b448d24f4cb8bfde6b21705ede1a |
| institution | Kabale University |
| issn | 2527-4384 2527-3442 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Cell and BioPharmaceutical Institute |
| record_format | Article |
| series | MCBS (Molecular and Cellular Biomedical Sciences) |
| spelling | doaj-art-9799b448d24f4cb8bfde6b21705ede1a2025-08-20T03:29:52ZengCell and BioPharmaceutical InstituteMCBS (Molecular and Cellular Biomedical Sciences)2527-43842527-34422025-07-01921152310.21705/mcbs.v9i2.635160Mesenchymal Stem Cell-Derived Exosomes Enhance FGF-1 and SDF-1 Expression in Rats with Second Degree BurnsNova Putri Hariani0Agung Putra1Prasetyowati Subchan2Eko Setiawan3Department of Postgraduate Biomedical Science, Faculty of Medicine, Universitas Islam Sultan Agung, SemarangStem Cell and Cancer Research (SCCR) Laboratory, Faculty of Medicine, Universitas Islam Sultan Agung, SemarangDepartment of Postgraduate Biomedical Science, Faculty of Medicine, Universitas Islam Sultan Agung, SemarangDepartment of Postgraduate Biomedical Science, Faculty of Medicine, Universitas Islam Sultan Agung, SemarangBackground: Second-degree burns cause extensive damage to the skin and pose significant health challenges, with current treatments facing limitations such as donor skin shortages and complications. Fibroblast growth factor 1 (FGF-1) and stromal-derived growth factor 1 (SDF-1) are critical for tissue repair. Emerging evidence suggests that mesenchymal stem cell-derived exosomes (E-MSCs) are a promising cell-free therapeutic option for enhancing wound healing through the modulation of FGF-1 and SDF-1. This study investigated the effect of E-MSCs on the expression of FGF-1 and SDF-1 genes in rats with second-degree burns. Materials and methods: This experimental study used a second-degree burn model in Wistar rats, treated with subcutaneous injections of E-MSCs at doses of 100 µL and 200 µL. Gene expression of FGF-1 and SDF-1 was quantified using qRT-PCR. Histological validation confirmed burn severity, and flow cytometry was used to characterize E-MSCs and exosomes. Results: An increase in FGF-1 and SDF-1 expression was observed in exosome-treated groups compared to the NaCL-treated group. The 200 µL E-MSCs-treated group showed the most significant enhancement in both growth factors, with statistically significant differences (p<0.05). These findings underline the efficacy of E-MSCs in modulating critical genes involved in wound healing. Conclusion: E-MSCs significantly upregulate FGF-1 and SDF-1 expression, promoting tissue repair and regeneration in second-degree burn models. This study highlights the potential of E-MSCs as a non-invasive therapeutic approach. Keywords: exosomes, FGF-1, mesenchymal stem cells, SDF-1https://cellbiopharm.com/ojs/index.php/MCBS/article/view/635 |
| spellingShingle | Nova Putri Hariani Agung Putra Prasetyowati Subchan Eko Setiawan Mesenchymal Stem Cell-Derived Exosomes Enhance FGF-1 and SDF-1 Expression in Rats with Second Degree Burns MCBS (Molecular and Cellular Biomedical Sciences) |
| title | Mesenchymal Stem Cell-Derived Exosomes Enhance FGF-1 and SDF-1 Expression in Rats with Second Degree Burns |
| title_full | Mesenchymal Stem Cell-Derived Exosomes Enhance FGF-1 and SDF-1 Expression in Rats with Second Degree Burns |
| title_fullStr | Mesenchymal Stem Cell-Derived Exosomes Enhance FGF-1 and SDF-1 Expression in Rats with Second Degree Burns |
| title_full_unstemmed | Mesenchymal Stem Cell-Derived Exosomes Enhance FGF-1 and SDF-1 Expression in Rats with Second Degree Burns |
| title_short | Mesenchymal Stem Cell-Derived Exosomes Enhance FGF-1 and SDF-1 Expression in Rats with Second Degree Burns |
| title_sort | mesenchymal stem cell derived exosomes enhance fgf 1 and sdf 1 expression in rats with second degree burns |
| url | https://cellbiopharm.com/ojs/index.php/MCBS/article/view/635 |
| work_keys_str_mv | AT novaputrihariani mesenchymalstemcellderivedexosomesenhancefgf1andsdf1expressioninratswithseconddegreeburns AT agungputra mesenchymalstemcellderivedexosomesenhancefgf1andsdf1expressioninratswithseconddegreeburns AT prasetyowatisubchan mesenchymalstemcellderivedexosomesenhancefgf1andsdf1expressioninratswithseconddegreeburns AT ekosetiawan mesenchymalstemcellderivedexosomesenhancefgf1andsdf1expressioninratswithseconddegreeburns |