All clinically-relevant blood components transmit prion disease following a single blood transfusion: a sheep model of vCJD.

Variant CJD (vCJD) is an incurable, infectious human disease, likely arising from the consumption of BSE-contaminated meat products. Whilst the epidemic appears to be waning, there is much concern that vCJD infection may be perpetuated in humans by the transfusion of contaminated blood products. Sin...

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Main Authors: Sandra McCutcheon, Anthony Richard Alejo Blanco, E Fiona Houston, Christopher de Wolf, Boon Chin Tan, Antony Smith, Martin H Groschup, Nora Hunter, Valerie S Hornsey, Ian R MacGregor, Christopher V Prowse, Marc Turner, Jean C Manson
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0023169&type=printable
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author Sandra McCutcheon
Anthony Richard Alejo Blanco
E Fiona Houston
Christopher de Wolf
Boon Chin Tan
Antony Smith
Martin H Groschup
Nora Hunter
Valerie S Hornsey
Ian R MacGregor
Christopher V Prowse
Marc Turner
Jean C Manson
author_facet Sandra McCutcheon
Anthony Richard Alejo Blanco
E Fiona Houston
Christopher de Wolf
Boon Chin Tan
Antony Smith
Martin H Groschup
Nora Hunter
Valerie S Hornsey
Ian R MacGregor
Christopher V Prowse
Marc Turner
Jean C Manson
author_sort Sandra McCutcheon
collection DOAJ
description Variant CJD (vCJD) is an incurable, infectious human disease, likely arising from the consumption of BSE-contaminated meat products. Whilst the epidemic appears to be waning, there is much concern that vCJD infection may be perpetuated in humans by the transfusion of contaminated blood products. Since 2004, several cases of transfusion-associated vCJD transmission have been reported and linked to blood collected from pre-clinically affected donors. Using an animal model in which the disease manifested resembles that of humans affected with vCJD, we examined which blood components used in human medicine are likely to pose the greatest risk of transmitting vCJD via transfusion. We collected two full units of blood from BSE-infected donor animals during the pre-clinical phase of infection. Using methods employed by transfusion services we prepared red cell concentrates, plasma and platelets units (including leucoreduced equivalents). Following transfusion, we showed that all components contain sufficient levels of infectivity to cause disease following only a single transfusion and also that leucoreduction did not prevent disease transmission. These data suggest that all blood components are vectors for prion disease transmission, and highlight the importance of multiple control measures to minimise the risk of human to human transmission of vCJD by blood transfusion.
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spelling doaj-art-9787d6f5dbc047c7b4ad98b09f517ea92025-08-20T02:30:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0168e2316910.1371/journal.pone.0023169All clinically-relevant blood components transmit prion disease following a single blood transfusion: a sheep model of vCJD.Sandra McCutcheonAnthony Richard Alejo BlancoE Fiona HoustonChristopher de WolfBoon Chin TanAntony SmithMartin H GroschupNora HunterValerie S HornseyIan R MacGregorChristopher V ProwseMarc TurnerJean C MansonVariant CJD (vCJD) is an incurable, infectious human disease, likely arising from the consumption of BSE-contaminated meat products. Whilst the epidemic appears to be waning, there is much concern that vCJD infection may be perpetuated in humans by the transfusion of contaminated blood products. Since 2004, several cases of transfusion-associated vCJD transmission have been reported and linked to blood collected from pre-clinically affected donors. Using an animal model in which the disease manifested resembles that of humans affected with vCJD, we examined which blood components used in human medicine are likely to pose the greatest risk of transmitting vCJD via transfusion. We collected two full units of blood from BSE-infected donor animals during the pre-clinical phase of infection. Using methods employed by transfusion services we prepared red cell concentrates, plasma and platelets units (including leucoreduced equivalents). Following transfusion, we showed that all components contain sufficient levels of infectivity to cause disease following only a single transfusion and also that leucoreduction did not prevent disease transmission. These data suggest that all blood components are vectors for prion disease transmission, and highlight the importance of multiple control measures to minimise the risk of human to human transmission of vCJD by blood transfusion.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0023169&type=printable
spellingShingle Sandra McCutcheon
Anthony Richard Alejo Blanco
E Fiona Houston
Christopher de Wolf
Boon Chin Tan
Antony Smith
Martin H Groschup
Nora Hunter
Valerie S Hornsey
Ian R MacGregor
Christopher V Prowse
Marc Turner
Jean C Manson
All clinically-relevant blood components transmit prion disease following a single blood transfusion: a sheep model of vCJD.
PLoS ONE
title All clinically-relevant blood components transmit prion disease following a single blood transfusion: a sheep model of vCJD.
title_full All clinically-relevant blood components transmit prion disease following a single blood transfusion: a sheep model of vCJD.
title_fullStr All clinically-relevant blood components transmit prion disease following a single blood transfusion: a sheep model of vCJD.
title_full_unstemmed All clinically-relevant blood components transmit prion disease following a single blood transfusion: a sheep model of vCJD.
title_short All clinically-relevant blood components transmit prion disease following a single blood transfusion: a sheep model of vCJD.
title_sort all clinically relevant blood components transmit prion disease following a single blood transfusion a sheep model of vcjd
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0023169&type=printable
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