Detection of Human Papillomavirus Type 2 Related Sequence in Oral Papilloma
Oral papilloma is a benign tumourous lesion. Part of this lesion is associated with human papillomavirus (HPV) infection. We analysed the genetical and histopathological evidence for HPV type 2 infection in three oral papillomas. Southern blot hybridization showed HPV 2a sequence in one lesion. Cell...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
1998-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/1998/705216 |
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| _version_ | 1832563106314715136 |
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| author | Taihei Yamaguchi Masanobu Shindoh Akira Amemiya Nobuo Inoue Masaaki Kawamura Hiroshi Sakaoka Masakazu Inoue Kei Fujinaga |
| author_facet | Taihei Yamaguchi Masanobu Shindoh Akira Amemiya Nobuo Inoue Masaaki Kawamura Hiroshi Sakaoka Masakazu Inoue Kei Fujinaga |
| author_sort | Taihei Yamaguchi |
| collection | DOAJ |
| description | Oral papilloma is a benign tumourous lesion. Part of this lesion is associated with human papillomavirus (HPV) infection. We analysed the genetical and histopathological evidence for HPV type 2 infection in three oral papillomas. Southern blot hybridization showed HPV 2a sequence in one lesion. Cells of the positive specimen appeared to contain high copy numbers of the viral DNA in an episomal state. In situ staining demonstrated virus capsid antigen in koilocytotic cells and surrounding cells in the hyperplastic epithelial layer. Two other specimens contained no HPV sequences by labeled probe of full length linear HPVs 2a, 6b, 11, 16, 18, 31 and 33 DNA under low stringency hybridization conditions. These results showed the possibility that HPV 2 plays a role in oral papilloma. |
| format | Article |
| id | doaj-art-9772003c7c79440fb22aed0546c31f24 |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-9772003c7c79440fb22aed0546c31f242025-02-03T01:21:01ZengWileyAnalytical Cellular Pathology0921-89121878-36511998-01-0116312513010.1155/1998/705216Detection of Human Papillomavirus Type 2 Related Sequence in Oral PapillomaTaihei Yamaguchi0Masanobu Shindoh1Akira Amemiya2Nobuo Inoue3Masaaki Kawamura4Hiroshi Sakaoka5Masakazu Inoue6Kei Fujinaga7Department of Preventive Dentistry, Kagoshima University Dental School, Kagoshima, JapanDepartment of Oral Pathology, Hokkaido University Dental School, Sapporo, JapanDepartment of Oral Pathology, Hokkaido University Dental School, Sapporo, JapanDepartment of Oral Surgery, Hokkaido University Dental School, Sapporo, JapanDepartment of Oral Surgery, Hokkaido University Dental School, Sapporo, JapanDepartment of Oral Microbiology, Hokkaido University Dental School, Sapporo, JapanDepartment of Preventive Dentistry, Kagoshima University Dental School, Kagoshima, JapanDepartment of Molecular Biology, Sapporo Medical College, Sapporo, JapanOral papilloma is a benign tumourous lesion. Part of this lesion is associated with human papillomavirus (HPV) infection. We analysed the genetical and histopathological evidence for HPV type 2 infection in three oral papillomas. Southern blot hybridization showed HPV 2a sequence in one lesion. Cells of the positive specimen appeared to contain high copy numbers of the viral DNA in an episomal state. In situ staining demonstrated virus capsid antigen in koilocytotic cells and surrounding cells in the hyperplastic epithelial layer. Two other specimens contained no HPV sequences by labeled probe of full length linear HPVs 2a, 6b, 11, 16, 18, 31 and 33 DNA under low stringency hybridization conditions. These results showed the possibility that HPV 2 plays a role in oral papilloma.http://dx.doi.org/10.1155/1998/705216 |
| spellingShingle | Taihei Yamaguchi Masanobu Shindoh Akira Amemiya Nobuo Inoue Masaaki Kawamura Hiroshi Sakaoka Masakazu Inoue Kei Fujinaga Detection of Human Papillomavirus Type 2 Related Sequence in Oral Papilloma Analytical Cellular Pathology |
| title | Detection of Human Papillomavirus Type 2 Related Sequence in Oral Papilloma |
| title_full | Detection of Human Papillomavirus Type 2 Related Sequence in Oral Papilloma |
| title_fullStr | Detection of Human Papillomavirus Type 2 Related Sequence in Oral Papilloma |
| title_full_unstemmed | Detection of Human Papillomavirus Type 2 Related Sequence in Oral Papilloma |
| title_short | Detection of Human Papillomavirus Type 2 Related Sequence in Oral Papilloma |
| title_sort | detection of human papillomavirus type 2 related sequence in oral papilloma |
| url | http://dx.doi.org/10.1155/1998/705216 |
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