Characterization of Vibrio parahaemolyticus isolated from clinical specimens and oysters in Thailand
Introduction: Vibrio parahaemolyticus is a common pathogen that can cause seafood-borne gastroenteritis in humans. We determined the prevalence and characteristics of V. parahaemolyticus isolated from clinical specimens and oysters in Thailand. Methodology: Isolates of V. parahaemolyticus from cl...
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The Journal of Infection in Developing Countries
2024-06-01
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| Series: | Journal of Infection in Developing Countries |
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| author | Nipaporn Tewawong Siriporn Kowaboot Sudaporn Kengkarn Unchana Thawornwan Pongrama Ramasoota Orasa Suthienkul |
| author_facet | Nipaporn Tewawong Siriporn Kowaboot Sudaporn Kengkarn Unchana Thawornwan Pongrama Ramasoota Orasa Suthienkul |
| author_sort | Nipaporn Tewawong |
| collection | DOAJ |
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Introduction: Vibrio parahaemolyticus is a common pathogen that can cause seafood-borne gastroenteritis in humans. We determined the prevalence and characteristics of V. parahaemolyticus isolated from clinical specimens and oysters in Thailand.
Methodology: Isolates of V. parahaemolyticus from clinical specimens (n = 77) and oysters (n = 224) were identified by biochemical testing, polymerase chain reaction (PCR) assays, and serotyping. The toxin genes, antimicrobial resistance, and β-lactamase production were determined.
Results: A total of 301 isolates were confirmed as V. parahaemolyticus by PCR using specific primers for the toxR gene. The majority of clinical isolates carried the tdh+/trh− genotype (82.1%), and one of each isolate was tdh−/trh+ and tdh+/trh+ genotypes. One isolate from oyster contained the tdh gene and another had the trh gene. Twenty-six serotypes were characterized among these isolates, and O3:K6 was the most common (37.7%), followed by OUT:KUT, and O4:K9. In 2010, most clinical and oyster isolates were susceptible to antibiotics, with the exception of ampicillin. In 2012, clinical isolates were not susceptible to cephalothin (52.4%), streptomycin (95.2%), amikacin (66.6%), kanamycin (61.9%), and erythromycin (95.2%), significantly more frequently than in 2010. More than 95% of isolates that were not susceptible to ampicillin produced β-lactamase enzymes.
Conclusions: We found toxin genes in two oyster isolates, and the clinical isolates that were initially determined to be resistant to several antibiotics. Toxin genes and antimicrobial susceptibility profiles of V. parahaemolyticus from seafood and environment should be continually monitored to determine the spread of toxin and antimicrobial resistance genes.
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| format | Article |
| id | doaj-art-9758a4ffcd664bbeb6f5d6192c6975dc |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-9758a4ffcd664bbeb6f5d6192c6975dc2025-08-20T02:27:14ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802024-06-01180610.3855/jidc.18470Characterization of Vibrio parahaemolyticus isolated from clinical specimens and oysters in ThailandNipaporn Tewawong0Siriporn Kowaboot1Sudaporn Kengkarn2Unchana Thawornwan3Pongrama Ramasoota4Orasa Suthienkul5Faculty of Medical Technology, Rangsit University, Pathum Thani, ThailandFaculty of Medical Technology, Rangsit University, Pathum Thani, ThailandFaculty of Medical Technology, Rangsit University, Pathum Thani, ThailandBamrasnaradura Infectious Diseases Institute, Ministry of Public Health, Nonthaburi, ThailandDepartment of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, ThailandFaculty of Public Health, Mahidol University, Bangkok, Thailand Introduction: Vibrio parahaemolyticus is a common pathogen that can cause seafood-borne gastroenteritis in humans. We determined the prevalence and characteristics of V. parahaemolyticus isolated from clinical specimens and oysters in Thailand. Methodology: Isolates of V. parahaemolyticus from clinical specimens (n = 77) and oysters (n = 224) were identified by biochemical testing, polymerase chain reaction (PCR) assays, and serotyping. The toxin genes, antimicrobial resistance, and β-lactamase production were determined. Results: A total of 301 isolates were confirmed as V. parahaemolyticus by PCR using specific primers for the toxR gene. The majority of clinical isolates carried the tdh+/trh− genotype (82.1%), and one of each isolate was tdh−/trh+ and tdh+/trh+ genotypes. One isolate from oyster contained the tdh gene and another had the trh gene. Twenty-six serotypes were characterized among these isolates, and O3:K6 was the most common (37.7%), followed by OUT:KUT, and O4:K9. In 2010, most clinical and oyster isolates were susceptible to antibiotics, with the exception of ampicillin. In 2012, clinical isolates were not susceptible to cephalothin (52.4%), streptomycin (95.2%), amikacin (66.6%), kanamycin (61.9%), and erythromycin (95.2%), significantly more frequently than in 2010. More than 95% of isolates that were not susceptible to ampicillin produced β-lactamase enzymes. Conclusions: We found toxin genes in two oyster isolates, and the clinical isolates that were initially determined to be resistant to several antibiotics. Toxin genes and antimicrobial susceptibility profiles of V. parahaemolyticus from seafood and environment should be continually monitored to determine the spread of toxin and antimicrobial resistance genes. https://jidc.org/index.php/journal/article/view/18470Vibrio parahaemolyticusserotypeoysterThailand |
| spellingShingle | Nipaporn Tewawong Siriporn Kowaboot Sudaporn Kengkarn Unchana Thawornwan Pongrama Ramasoota Orasa Suthienkul Characterization of Vibrio parahaemolyticus isolated from clinical specimens and oysters in Thailand Journal of Infection in Developing Countries Vibrio parahaemolyticus serotype oyster Thailand |
| title | Characterization of Vibrio parahaemolyticus isolated from clinical specimens and oysters in Thailand |
| title_full | Characterization of Vibrio parahaemolyticus isolated from clinical specimens and oysters in Thailand |
| title_fullStr | Characterization of Vibrio parahaemolyticus isolated from clinical specimens and oysters in Thailand |
| title_full_unstemmed | Characterization of Vibrio parahaemolyticus isolated from clinical specimens and oysters in Thailand |
| title_short | Characterization of Vibrio parahaemolyticus isolated from clinical specimens and oysters in Thailand |
| title_sort | characterization of vibrio parahaemolyticus isolated from clinical specimens and oysters in thailand |
| topic | Vibrio parahaemolyticus serotype oyster Thailand |
| url | https://jidc.org/index.php/journal/article/view/18470 |
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