Decreased expression of hsa_circ_0001895 in human gastric cancer and its clinical significances

Circular RNAs are a special class of endogenous RNAs characterized by jointing 3′ and 5′ ends together via exon or intron circularization. Recent studies found that circular RNAs are involved in the development of some human diseases. However, little is known about their roles in human gastric cance...

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Main Authors: Yongfu Shao, Linbo Chen, Rongdan Lu, Xinjun Zhang, Bingxiu Xiao, Guoliang Ye, Junming Guo
Format: Article
Language:English
Published: SAGE Publishing 2017-04-01
Series:Tumor Biology
Online Access:https://doi.org/10.1177/1010428317699125
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author Yongfu Shao
Linbo Chen
Rongdan Lu
Xinjun Zhang
Bingxiu Xiao
Guoliang Ye
Junming Guo
author_facet Yongfu Shao
Linbo Chen
Rongdan Lu
Xinjun Zhang
Bingxiu Xiao
Guoliang Ye
Junming Guo
author_sort Yongfu Shao
collection DOAJ
description Circular RNAs are a special class of endogenous RNAs characterized by jointing 3′ and 5′ ends together via exon or intron circularization. Recent studies found that circular RNAs are involved in the development of some human diseases. However, little is known about their roles in human gastric cancer. In this study, we chose hsa_circ_0001895 as a targeted circRNA to investigate its clinical significances in gastric cancer patients. Hsa_circ_0001895 expression levels in five gastric cancer cell lines and 257 specimens of tissues were measured by real-time quantitative reverse transcription polymerase chain reaction. Then, the potential relationship between hsa_circ_0001895 expression levels and patients’ clinicopathological factors was investigated. A receiver operating characteristic curve was constructed for evaluating the diagnostic value of hsa_circ_0001895. Hsa_circ_0001895 expression levels in five detected gastric cancer cell lines (AGS, BGC-823, HGC-27, MGC-803, and SGC-7901) were all significantly downregulated than those in normal gastric epithelial GES-1 cells. Besides, compared with healthy control tissues, it was downregulated not only in 69.8% (67/96) gastric cancer tissues but also in gastric precancerous lesions. Moreover, hsa_circ_0001895 expression levels were significantly correlated with cell differentiation, Borrmann type, and tissue carcino-embryonic antigen expression. Our results suggested that hsa_circ_0001895 may play crucial roles during gastric cancerogenesis and is a potential biomarker for clinical prognosis prediction.
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spelling doaj-art-9750b08fe836438299ba8f7ea1eb076f2025-08-20T02:52:24ZengSAGE PublishingTumor Biology1423-03802017-04-013910.1177/1010428317699125Decreased expression of hsa_circ_0001895 in human gastric cancer and its clinical significancesYongfu Shao0Linbo Chen1Rongdan Lu2Xinjun Zhang3Bingxiu Xiao4Guoliang Ye5Junming Guo6Department of Gastroenterology, The Affiliated Hospital of Ningbo University School of Medicine, Ningbo, ChinaDepartment of Biochemistry and Molecular Biology and Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, ChinaDepartment of Biochemistry and Molecular Biology and Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, ChinaDepartment of Gastroenterology, The Affiliated Hospital of Ningbo University School of Medicine, Ningbo, ChinaDepartment of Biochemistry and Molecular Biology and Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, ChinaDepartment of Gastroenterology, The Affiliated Hospital of Ningbo University School of Medicine, Ningbo, ChinaDepartment of Biochemistry and Molecular Biology and Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, ChinaCircular RNAs are a special class of endogenous RNAs characterized by jointing 3′ and 5′ ends together via exon or intron circularization. Recent studies found that circular RNAs are involved in the development of some human diseases. However, little is known about their roles in human gastric cancer. In this study, we chose hsa_circ_0001895 as a targeted circRNA to investigate its clinical significances in gastric cancer patients. Hsa_circ_0001895 expression levels in five gastric cancer cell lines and 257 specimens of tissues were measured by real-time quantitative reverse transcription polymerase chain reaction. Then, the potential relationship between hsa_circ_0001895 expression levels and patients’ clinicopathological factors was investigated. A receiver operating characteristic curve was constructed for evaluating the diagnostic value of hsa_circ_0001895. Hsa_circ_0001895 expression levels in five detected gastric cancer cell lines (AGS, BGC-823, HGC-27, MGC-803, and SGC-7901) were all significantly downregulated than those in normal gastric epithelial GES-1 cells. Besides, compared with healthy control tissues, it was downregulated not only in 69.8% (67/96) gastric cancer tissues but also in gastric precancerous lesions. Moreover, hsa_circ_0001895 expression levels were significantly correlated with cell differentiation, Borrmann type, and tissue carcino-embryonic antigen expression. Our results suggested that hsa_circ_0001895 may play crucial roles during gastric cancerogenesis and is a potential biomarker for clinical prognosis prediction.https://doi.org/10.1177/1010428317699125
spellingShingle Yongfu Shao
Linbo Chen
Rongdan Lu
Xinjun Zhang
Bingxiu Xiao
Guoliang Ye
Junming Guo
Decreased expression of hsa_circ_0001895 in human gastric cancer and its clinical significances
Tumor Biology
title Decreased expression of hsa_circ_0001895 in human gastric cancer and its clinical significances
title_full Decreased expression of hsa_circ_0001895 in human gastric cancer and its clinical significances
title_fullStr Decreased expression of hsa_circ_0001895 in human gastric cancer and its clinical significances
title_full_unstemmed Decreased expression of hsa_circ_0001895 in human gastric cancer and its clinical significances
title_short Decreased expression of hsa_circ_0001895 in human gastric cancer and its clinical significances
title_sort decreased expression of hsa circ 0001895 in human gastric cancer and its clinical significances
url https://doi.org/10.1177/1010428317699125
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