Alkyl chain length-regulated in situ intelligent nano-assemblies with AIE-active photosensitizers for photodynamic cancer therapy

Photodynamic therapy (PDT) brings new hope for the treatment of breast cancer due to few side effects and highly effective cell killing; however, the low bioavailability of traditional photosensitizers (PSs) and their dependence on oxygen severely limits their application. Aggregation-induced emissi...

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Main Authors: Lingyi Shen, Qilong Zhang, Yongchao Yao, Yali Huang, Zhichang Zheng, Ming Li, Hong Xu, Lin Tan, Xukun Liao, Binyi Xia, Lin Li, Carl Redshaw, Yang Bai, Chengli Yang
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Asian Journal of Pharmaceutical Sciences
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Online Access:http://www.sciencedirect.com/science/article/pii/S1818087624000849
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author Lingyi Shen
Qilong Zhang
Yongchao Yao
Yali Huang
Zhichang Zheng
Ming Li
Hong Xu
Lin Tan
Xukun Liao
Binyi Xia
Lin Li
Carl Redshaw
Yang Bai
Chengli Yang
author_facet Lingyi Shen
Qilong Zhang
Yongchao Yao
Yali Huang
Zhichang Zheng
Ming Li
Hong Xu
Lin Tan
Xukun Liao
Binyi Xia
Lin Li
Carl Redshaw
Yang Bai
Chengli Yang
author_sort Lingyi Shen
collection DOAJ
description Photodynamic therapy (PDT) brings new hope for the treatment of breast cancer due to few side effects and highly effective cell killing; however, the low bioavailability of traditional photosensitizers (PSs) and their dependence on oxygen severely limits their application. Aggregation-induced emission (AIE) PSs can dramatically facilitate the photosensitization effect, which can have positive impacts on tumor PDT. To-date, most AIE PSs lack tumor targeting capability and possess poor cell delivery, resulting in their use in large quantities that are harmful to healthy tissues. In this study, a series of AIE PSs based on pyridinium-substituted triphenylamine salts (TTPAs 1–6) with different alkyl chain lengths are synthesized. Results reveal that TTPAs 1–6 promote the generation of type I and II ROS, including ·OH and 1O2. In particular, the membrane permeability and targeting of TTPAs 4-6 bearing C8-C10 side-chains are higher than TTPAs 1-3 bearing shorter alkyl chains. Additionally, they can assemble with albumin, thereby forming nanoparticles (TTPA 4–6 NPs) in situ in blood, which significantly facilitates mitochondrial-targeting and strong ROS generation ability. Moreover, the TTPA 4–6 NPs are pH-responsive, allowing for increased accumulation or endocytosis of the tumor and enhancing the imaging or therapeutic effect. Therefore, the in vivo distributions of TTPA 4–6 NPs are visually enriched in tumor sites and exhibited excellent PDT efficacy. This work demonstrates a novel strategy for AIE PDT and has the potential to play an essential role in clinical applications using nano-delivery systems.
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spelling doaj-art-97229dcfe56d49f296d18cac320b0b4f2024-12-25T04:21:01ZengElsevierAsian Journal of Pharmaceutical Sciences1818-08762024-12-01196100967Alkyl chain length-regulated in situ intelligent nano-assemblies with AIE-active photosensitizers for photodynamic cancer therapyLingyi Shen0Qilong Zhang1Yongchao Yao2Yali Huang3Zhichang Zheng4Ming Li5Hong Xu6Lin Tan7Xukun Liao8Binyi Xia9Lin Li10Carl Redshaw11Yang Bai12Chengli Yang13School of Basic Medicine, Guizhou Medical University, Guiyang 550025, ChinaSchool of Basic Medicine, Guizhou Medical University, Guiyang 550025, ChinaDepartment of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, ChinaSchool of Basic Medicine, Guizhou Medical University, Guiyang 550025, ChinaDepartment of Pharmacy, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, ChinaDepartment of Pharmacy, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, ChinaSchool of Basic Medicine, Guizhou Medical University, Guiyang 550025, ChinaDepartment of Pharmacy, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, ChinaDepartment of Pharmacy, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, ChinaDepartment of Pharmacy, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, ChinaDepartment of Pharmacy, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, ChinaChemistry, School of Natural Sciences, University of Hull, Yorkshire HU6 7RX, UKDepartment of Pharmacy, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, ChinaDepartment of Pharmacy, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China; Clinical Research Center, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China; Corresponding author.Photodynamic therapy (PDT) brings new hope for the treatment of breast cancer due to few side effects and highly effective cell killing; however, the low bioavailability of traditional photosensitizers (PSs) and their dependence on oxygen severely limits their application. Aggregation-induced emission (AIE) PSs can dramatically facilitate the photosensitization effect, which can have positive impacts on tumor PDT. To-date, most AIE PSs lack tumor targeting capability and possess poor cell delivery, resulting in their use in large quantities that are harmful to healthy tissues. In this study, a series of AIE PSs based on pyridinium-substituted triphenylamine salts (TTPAs 1–6) with different alkyl chain lengths are synthesized. Results reveal that TTPAs 1–6 promote the generation of type I and II ROS, including ·OH and 1O2. In particular, the membrane permeability and targeting of TTPAs 4-6 bearing C8-C10 side-chains are higher than TTPAs 1-3 bearing shorter alkyl chains. Additionally, they can assemble with albumin, thereby forming nanoparticles (TTPA 4–6 NPs) in situ in blood, which significantly facilitates mitochondrial-targeting and strong ROS generation ability. Moreover, the TTPA 4–6 NPs are pH-responsive, allowing for increased accumulation or endocytosis of the tumor and enhancing the imaging or therapeutic effect. Therefore, the in vivo distributions of TTPA 4–6 NPs are visually enriched in tumor sites and exhibited excellent PDT efficacy. This work demonstrates a novel strategy for AIE PDT and has the potential to play an essential role in clinical applications using nano-delivery systems.http://www.sciencedirect.com/science/article/pii/S1818087624000849Aggregation-induced emissionPhotosensitizersPhotodynamic therapyROS generationSelf-assembled nanoparticles
spellingShingle Lingyi Shen
Qilong Zhang
Yongchao Yao
Yali Huang
Zhichang Zheng
Ming Li
Hong Xu
Lin Tan
Xukun Liao
Binyi Xia
Lin Li
Carl Redshaw
Yang Bai
Chengli Yang
Alkyl chain length-regulated in situ intelligent nano-assemblies with AIE-active photosensitizers for photodynamic cancer therapy
Asian Journal of Pharmaceutical Sciences
Aggregation-induced emission
Photosensitizers
Photodynamic therapy
ROS generation
Self-assembled nanoparticles
title Alkyl chain length-regulated in situ intelligent nano-assemblies with AIE-active photosensitizers for photodynamic cancer therapy
title_full Alkyl chain length-regulated in situ intelligent nano-assemblies with AIE-active photosensitizers for photodynamic cancer therapy
title_fullStr Alkyl chain length-regulated in situ intelligent nano-assemblies with AIE-active photosensitizers for photodynamic cancer therapy
title_full_unstemmed Alkyl chain length-regulated in situ intelligent nano-assemblies with AIE-active photosensitizers for photodynamic cancer therapy
title_short Alkyl chain length-regulated in situ intelligent nano-assemblies with AIE-active photosensitizers for photodynamic cancer therapy
title_sort alkyl chain length regulated in situ intelligent nano assemblies with aie active photosensitizers for photodynamic cancer therapy
topic Aggregation-induced emission
Photosensitizers
Photodynamic therapy
ROS generation
Self-assembled nanoparticles
url http://www.sciencedirect.com/science/article/pii/S1818087624000849
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