The Effect of Low-Level Laser Therapy on the Expression of 3β-HSD and StAR Genes in the Ovaries of NMRI Mice
Background and purpose: Ovulation induction increases the number and quality of oocytes, thereby enhancing fertility. However, it is associated with complications such as ovarian hyperstimulation syndrome, apoptosis, and oxidative stress, which may negatively affect oocyte quality. Low-level laser t...
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Mazandaran University of Medical Sciences
2025-08-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
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| Online Access: | http://jmums.mazums.ac.ir/article-1-21794-en.pdf |
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| author | Mehdi Aghamardi Mitra Barzroodipour Nastaran Azarbarz Mohammadreza Bayatiani Mohammad Bayat |
| author_facet | Mehdi Aghamardi Mitra Barzroodipour Nastaran Azarbarz Mohammadreza Bayatiani Mohammad Bayat |
| author_sort | Mehdi Aghamardi |
| collection | DOAJ |
| description | Background and purpose: Ovulation induction increases the number and quality of oocytes, thereby enhancing fertility. However, it is associated with complications such as ovarian hyperstimulation syndrome, apoptosis, and oxidative stress, which may negatively affect oocyte quality. Low-level laser therapy (LLLT), by modulating cellular activity and increasing the expression of genes involved in steroidogenesis, has the potential to improve ovarian function. This study aimed to investigate the effect of LLLT on the expression of 3β-HSD and StAR genes involved in the steroidogenesis pathway, as well as on the number and quality of oocytes and the histological features of ovaries in female NMRI mice.
Materials and methods: In this experimental study, 32 adults female NMRI mice were divided into four groups: control, laser, PMSG, and PMSG & laser. Laser irradiation with a wavelength of 808 nm and a power of 60 mW was applied to the ovaries three times weekly for 21 days. After treatment, ovaries were collected for histological examination and gene expression analysis using real-time PCR. Additionally, the number and quality of oocytes were evaluated, and the data were statistically analyzed.
Results: The results showed that the size of primary and secondary follicles, as well as the expression of 3β-HSD and StAR genes, significantly increased in the PMSG and combined PMSG & laser groups compared to the control and laser groups. The number of atretic follicles significantly decreased in the combined PMSG & laser group compared to the control and PMSG groups. The size of Graafian follicles was significantly larger in the combined group compared to the PMSG and laser groups. Oocyte quality was improved in both the PMSG and combined PMSG & laser groups.
Conclusion: Low-level laser therapy combined with ovulation induction significantly increases the expression of key steroidogenesis genes and improves ovarian histological parameters, thereby enhancing the number and quality of oocytes and overall ovarian function. Therefore, LLLT can be considered a promising complementary approach in infertility treatment. |
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| id | doaj-art-971cf9158dd64167a068e305c5bd0285 |
| institution | Kabale University |
| issn | 1735-9260 1735-9279 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Mazandaran University of Medical Sciences |
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| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj-art-971cf9158dd64167a068e305c5bd02852025-08-20T05:26:28ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792025-08-01352472739The Effect of Low-Level Laser Therapy on the Expression of 3β-HSD and StAR Genes in the Ovaries of NMRI MiceMehdi Aghamardi0Mitra Barzroodipour1Nastaran Azarbarz2Mohammadreza Bayatiani3Mohammad Bayat4 MSc in Anatomical Sciences, Students Research Committee, Arak University of Medical Sciences, Arak, Iran Assistant Professor, Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran Assistant Professor, Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran Associate Professor, Department of Medical Physics and Radiotherapy, Arak University of Medical Sciences, Arak, Iran Assistant Professor, Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran Background and purpose: Ovulation induction increases the number and quality of oocytes, thereby enhancing fertility. However, it is associated with complications such as ovarian hyperstimulation syndrome, apoptosis, and oxidative stress, which may negatively affect oocyte quality. Low-level laser therapy (LLLT), by modulating cellular activity and increasing the expression of genes involved in steroidogenesis, has the potential to improve ovarian function. This study aimed to investigate the effect of LLLT on the expression of 3β-HSD and StAR genes involved in the steroidogenesis pathway, as well as on the number and quality of oocytes and the histological features of ovaries in female NMRI mice. Materials and methods: In this experimental study, 32 adults female NMRI mice were divided into four groups: control, laser, PMSG, and PMSG & laser. Laser irradiation with a wavelength of 808 nm and a power of 60 mW was applied to the ovaries three times weekly for 21 days. After treatment, ovaries were collected for histological examination and gene expression analysis using real-time PCR. Additionally, the number and quality of oocytes were evaluated, and the data were statistically analyzed. Results: The results showed that the size of primary and secondary follicles, as well as the expression of 3β-HSD and StAR genes, significantly increased in the PMSG and combined PMSG & laser groups compared to the control and laser groups. The number of atretic follicles significantly decreased in the combined PMSG & laser group compared to the control and PMSG groups. The size of Graafian follicles was significantly larger in the combined group compared to the PMSG and laser groups. Oocyte quality was improved in both the PMSG and combined PMSG & laser groups. Conclusion: Low-level laser therapy combined with ovulation induction significantly increases the expression of key steroidogenesis genes and improves ovarian histological parameters, thereby enhancing the number and quality of oocytes and overall ovarian function. Therefore, LLLT can be considered a promising complementary approach in infertility treatment.http://jmums.mazums.ac.ir/article-1-21794-en.pdflow-level laser therapysteroidogenesisovary3βhsdstarovulation inductionnmri mouse |
| spellingShingle | Mehdi Aghamardi Mitra Barzroodipour Nastaran Azarbarz Mohammadreza Bayatiani Mohammad Bayat The Effect of Low-Level Laser Therapy on the Expression of 3β-HSD and StAR Genes in the Ovaries of NMRI Mice Journal of Mazandaran University of Medical Sciences low-level laser therapy steroidogenesis ovary 3βhsd star ovulation induction nmri mouse |
| title | The Effect of Low-Level Laser Therapy on the Expression of 3β-HSD and StAR Genes in the Ovaries of NMRI Mice |
| title_full | The Effect of Low-Level Laser Therapy on the Expression of 3β-HSD and StAR Genes in the Ovaries of NMRI Mice |
| title_fullStr | The Effect of Low-Level Laser Therapy on the Expression of 3β-HSD and StAR Genes in the Ovaries of NMRI Mice |
| title_full_unstemmed | The Effect of Low-Level Laser Therapy on the Expression of 3β-HSD and StAR Genes in the Ovaries of NMRI Mice |
| title_short | The Effect of Low-Level Laser Therapy on the Expression of 3β-HSD and StAR Genes in the Ovaries of NMRI Mice |
| title_sort | effect of low level laser therapy on the expression of 3β hsd and star genes in the ovaries of nmri mice |
| topic | low-level laser therapy steroidogenesis ovary 3βhsd star ovulation induction nmri mouse |
| url | http://jmums.mazums.ac.ir/article-1-21794-en.pdf |
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