Localization and Upregulation of the Nasal Histamine H1 Receptor in Perennial Allergic Rhinitis
In the present study, we have investigated the expression of histamine H1 receptor in human turbinates by RT-PCR, western blotting, and immunohistochemistry. Human turbinates were obtained by turbinectomy from 12 patients with nasal obstruction refractory to medical therapy. RT-PCR analysis of total...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/951316 |
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| author | Hideaki Shirasaki Etsuko Kanaizumi Nobuhiko Seki Tetsuo Himi |
| author_facet | Hideaki Shirasaki Etsuko Kanaizumi Nobuhiko Seki Tetsuo Himi |
| author_sort | Hideaki Shirasaki |
| collection | DOAJ |
| description | In the present study, we have investigated the expression of histamine H1 receptor in human turbinates by RT-PCR, western blotting, and immunohistochemistry. Human turbinates were obtained by turbinectomy from 12 patients with nasal obstruction refractory to medical therapy. RT-PCR analysis of total RNA extracted from human nasal turbinate, primary cultured human nasal epithelial cells, and nasal vascular endothelial cells demonstrated the expression of histamine H1 receptor mRNA. About 56 kDa band was detected in human turbinates by western blot analysis using anti-H1 receptor antibody. The expression level of H1 receptor protein was marked in patients with nasal allergy than in patients with nonallergic rhinitis. The immunohistochemical study revealed that epithelial cells and vascular endothelial cells showed intense immunoreactivity for histamine H1 receptor. In addition, the blood vessels in superficial area expressed higher level of H1 receptor immunoreactivity than that in deeper area in the nasal mucosa. These results may have an important clinical implication for understanding the role of histamine H1 receptor on upper airway diseases such as allergic rhinitis and nonallergic rhinitis. |
| format | Article |
| id | doaj-art-971584ee22c34886b6bbdd9ca2e62e01 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-971584ee22c34886b6bbdd9ca2e62e012025-08-20T03:37:09ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/951316951316Localization and Upregulation of the Nasal Histamine H1 Receptor in Perennial Allergic RhinitisHideaki Shirasaki0Etsuko Kanaizumi1Nobuhiko Seki2Tetsuo Himi3Department of Otolaryngology, School of Medicine, Sapporo Medical University, S-1 W-16, Chu-ku, Sapporo 060-8543, JapanDepartment of Otolaryngology, School of Medicine, Sapporo Medical University, S-1 W-16, Chu-ku, Sapporo 060-8543, JapanDepartment of Otolaryngology, School of Medicine, Sapporo Medical University, S-1 W-16, Chu-ku, Sapporo 060-8543, JapanDepartment of Otolaryngology, School of Medicine, Sapporo Medical University, S-1 W-16, Chu-ku, Sapporo 060-8543, JapanIn the present study, we have investigated the expression of histamine H1 receptor in human turbinates by RT-PCR, western blotting, and immunohistochemistry. Human turbinates were obtained by turbinectomy from 12 patients with nasal obstruction refractory to medical therapy. RT-PCR analysis of total RNA extracted from human nasal turbinate, primary cultured human nasal epithelial cells, and nasal vascular endothelial cells demonstrated the expression of histamine H1 receptor mRNA. About 56 kDa band was detected in human turbinates by western blot analysis using anti-H1 receptor antibody. The expression level of H1 receptor protein was marked in patients with nasal allergy than in patients with nonallergic rhinitis. The immunohistochemical study revealed that epithelial cells and vascular endothelial cells showed intense immunoreactivity for histamine H1 receptor. In addition, the blood vessels in superficial area expressed higher level of H1 receptor immunoreactivity than that in deeper area in the nasal mucosa. These results may have an important clinical implication for understanding the role of histamine H1 receptor on upper airway diseases such as allergic rhinitis and nonallergic rhinitis.http://dx.doi.org/10.1155/2012/951316 |
| spellingShingle | Hideaki Shirasaki Etsuko Kanaizumi Nobuhiko Seki Tetsuo Himi Localization and Upregulation of the Nasal Histamine H1 Receptor in Perennial Allergic Rhinitis Mediators of Inflammation |
| title | Localization and Upregulation of the Nasal Histamine H1 Receptor in Perennial Allergic Rhinitis |
| title_full | Localization and Upregulation of the Nasal Histamine H1 Receptor in Perennial Allergic Rhinitis |
| title_fullStr | Localization and Upregulation of the Nasal Histamine H1 Receptor in Perennial Allergic Rhinitis |
| title_full_unstemmed | Localization and Upregulation of the Nasal Histamine H1 Receptor in Perennial Allergic Rhinitis |
| title_short | Localization and Upregulation of the Nasal Histamine H1 Receptor in Perennial Allergic Rhinitis |
| title_sort | localization and upregulation of the nasal histamine h1 receptor in perennial allergic rhinitis |
| url | http://dx.doi.org/10.1155/2012/951316 |
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