Antioxidant and cytokine modulation in PCOS rats protected with probiotics, myo-inositol, and herbal extracts
This study investigated the pathophysiology of polycystic ovarian syndrome (PCOS) and evaluated the protective effects of various treatments in immature female Wistar rats (N = 48). The rats were divided into 6 groups: Olive oil injection (negative control, G1); testosterone propionate (TP)-induced...
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Czech Academy of Agricultural Sciences
2025-06-01
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| Series: | Veterinární Medicína |
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| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-202506-0002_antioxidant-and-cytokine-modulation-in-pcos-rats-protected-with-probiotics-myo-inositol-and-herbal-extracts.php |
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| author | AA Zaki NM Alharbi TI Almundarij SM Albarrak |
| author_facet | AA Zaki NM Alharbi TI Almundarij SM Albarrak |
| author_sort | AA Zaki |
| collection | DOAJ |
| description | This study investigated the pathophysiology of polycystic ovarian syndrome (PCOS) and evaluated the protective effects of various treatments in immature female Wistar rats (N = 48). The rats were divided into 6 groups: Olive oil injection (negative control, G1); testosterone propionate (TP)-induced PCOS (G2); probiotic + TP (G3); myo-inositol (myo-ins) + TP (G4); U. dioica extract + TP (G5); W. somnifera extract + TP (G6). The body weight, body weight gain, and percentage gain were measured weekly and then transformed using the base-10 logarithm (log10). TNF-α, IL-4, IL-10, and IL-17 were weekly measured using ELISA kits. Glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) were analysed in the serum and liver extracts. The W. somnifera significantly reduced the TNF-α levels (P < 0.01). The probiotic and myo-ins significantly elevated the IL-10 levels (P < 0.01). Both plant extracts moderately restored the IL-10 levels. The probiotic and U. dioica administration significantly reduced the IL-17 levels (P < 0.01). The W. somnifera administration also decreased the IL-17 levels, though the effect was less pronounced than that of U. dioica. The probiotic, myo-ins, and W. somnifera groups exhibited enhanced CAT activity (P < 0.05). W. somnifera showed significant increases in the SOD and GSH-Px activities (P < 0.01), showing the most dramatic improvement. The use of these four treatments as a monotherapy in this study resulted in different changes. Therefore, further investigation is necessary to evaluate the protective effects of combining duos or trios of these treatments against this disease. |
| format | Article |
| id | doaj-art-971366efac9d4df8ae98f6bcb4f3bfcb |
| institution | DOAJ |
| issn | 0375-8427 1805-9392 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj-art-971366efac9d4df8ae98f6bcb4f3bfcb2025-08-20T02:46:23ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922025-06-0170620321710.17221/116/2024-VETMEDvet-202506-0002Antioxidant and cytokine modulation in PCOS rats protected with probiotics, myo-inositol, and herbal extractsAA Zaki0https://orcid.org/0000-0001-7616-0789NM Alharbi1https://orcid.org/0009-0006-1284-3090TI Almundarij2SM Albarrak3https://orcid.org/0000-0003-4965-6142Department of Biomedical Sciences, College of Veterinary Medicine, Qassim University, Buraydah, Saudi ArabiaNational Center for the Prevention & Control of Plants Pests & Animal Diseases, Buraydah, Qassim Region, Saudi ArabiaDepartment of Biomedical Sciences, College of Veterinary Medicine, Qassim University, Buraydah, Saudi ArabiaDepartment of Pathology and Laboratory Diagnosis, Collage of Veterinary Medicine, Qassim University, Buraydah, Saudi ArabiaThis study investigated the pathophysiology of polycystic ovarian syndrome (PCOS) and evaluated the protective effects of various treatments in immature female Wistar rats (N = 48). The rats were divided into 6 groups: Olive oil injection (negative control, G1); testosterone propionate (TP)-induced PCOS (G2); probiotic + TP (G3); myo-inositol (myo-ins) + TP (G4); U. dioica extract + TP (G5); W. somnifera extract + TP (G6). The body weight, body weight gain, and percentage gain were measured weekly and then transformed using the base-10 logarithm (log10). TNF-α, IL-4, IL-10, and IL-17 were weekly measured using ELISA kits. Glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) were analysed in the serum and liver extracts. The W. somnifera significantly reduced the TNF-α levels (P < 0.01). The probiotic and myo-ins significantly elevated the IL-10 levels (P < 0.01). Both plant extracts moderately restored the IL-10 levels. The probiotic and U. dioica administration significantly reduced the IL-17 levels (P < 0.01). The W. somnifera administration also decreased the IL-17 levels, though the effect was less pronounced than that of U. dioica. The probiotic, myo-ins, and W. somnifera groups exhibited enhanced CAT activity (P < 0.05). W. somnifera showed significant increases in the SOD and GSH-Px activities (P < 0.01), showing the most dramatic improvement. The use of these four treatments as a monotherapy in this study resulted in different changes. Therefore, further investigation is necessary to evaluate the protective effects of combining duos or trios of these treatments against this disease.https://vetmed.agriculturejournals.cz/artkey/vet-202506-0002_antioxidant-and-cytokine-modulation-in-pcos-rats-protected-with-probiotics-myo-inositol-and-herbal-extracts.phpantioxidantselisaimmunitymyo-inositolpolycystic ovarian syndrome |
| spellingShingle | AA Zaki NM Alharbi TI Almundarij SM Albarrak Antioxidant and cytokine modulation in PCOS rats protected with probiotics, myo-inositol, and herbal extracts Veterinární Medicína antioxidants elisa immunity myo-inositol polycystic ovarian syndrome |
| title | Antioxidant and cytokine modulation in PCOS rats protected with probiotics, myo-inositol, and herbal extracts |
| title_full | Antioxidant and cytokine modulation in PCOS rats protected with probiotics, myo-inositol, and herbal extracts |
| title_fullStr | Antioxidant and cytokine modulation in PCOS rats protected with probiotics, myo-inositol, and herbal extracts |
| title_full_unstemmed | Antioxidant and cytokine modulation in PCOS rats protected with probiotics, myo-inositol, and herbal extracts |
| title_short | Antioxidant and cytokine modulation in PCOS rats protected with probiotics, myo-inositol, and herbal extracts |
| title_sort | antioxidant and cytokine modulation in pcos rats protected with probiotics myo inositol and herbal extracts |
| topic | antioxidants elisa immunity myo-inositol polycystic ovarian syndrome |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-202506-0002_antioxidant-and-cytokine-modulation-in-pcos-rats-protected-with-probiotics-myo-inositol-and-herbal-extracts.php |
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