Comprehensive Genomic Analysis of Uropathogenic <i>E. coli</i>: Virulence Factors, Antimicrobial Resistance, and Mobile Genetic Elements
Our whole-genome sequencing analysis of sixteen uropathogenic <i>E. coli</i> isolates revealed a concerning picture of multidrug resistance and potentially virulent bacteria. All isolates belonged to four distinct clonal groups, with the highly prevalent ST131 lineage being associated wi...
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2024-09-01
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| author | Kidon Sung Mohamed Nawaz Miseon Park Jungwhan Chon Saeed A. Khan Khulud Alotaibi Ashraf A. Khan |
| author_facet | Kidon Sung Mohamed Nawaz Miseon Park Jungwhan Chon Saeed A. Khan Khulud Alotaibi Ashraf A. Khan |
| author_sort | Kidon Sung |
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| description | Our whole-genome sequencing analysis of sixteen uropathogenic <i>E. coli</i> isolates revealed a concerning picture of multidrug resistance and potentially virulent bacteria. All isolates belonged to four distinct clonal groups, with the highly prevalent ST131 lineage being associated with extensive antibiotic resistance and virulence factors. Notably, all isolates exhibited multidrug resistance, with some resistant to as many as 12 antibiotics. Fluoroquinolone resistance stemmed primarily from efflux pumps and mutations in gyrase and topoisomerase genes. Additionally, we identified genes encoding resistance to extended-spectrum cephalosporins, trimethoprim/sulfamethoxazole, and various heavy metals. The presence of diverse plasmids and phages suggests the potential for horizontal gene transfer and the dissemination of virulence factors. All isolates harbored genomic islands containing virulence factors associated with adhesion, biofilm formation, and invasion. Genes essential for iron acquisition, flagella biosynthesis, secretion systems, and toxin production were also prevalent. Adding further complexity to understanding the isolates’ genetic makeup, we identified CRISPR-Cas systems. This study underscores the need for continued genomic surveillance in understanding the pathogenic mechanisms and resistance profiles of uropathogenic <i>E. coli</i> to aid in developing targeted therapeutic strategies. |
| format | Article |
| id | doaj-art-96fb04f0982d45fa95c8c44b003f29ab |
| institution | OA Journals |
| issn | 2076-0817 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
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| series | Pathogens |
| spelling | doaj-art-96fb04f0982d45fa95c8c44b003f29ab2025-08-20T01:55:45ZengMDPI AGPathogens2076-08172024-09-0113979410.3390/pathogens13090794Comprehensive Genomic Analysis of Uropathogenic <i>E. coli</i>: Virulence Factors, Antimicrobial Resistance, and Mobile Genetic ElementsKidon Sung0Mohamed Nawaz1Miseon Park2Jungwhan Chon3Saeed A. Khan4Khulud Alotaibi5Ashraf A. Khan6Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USADivision of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USADivision of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USADepartment of Companion Animal Health, Inje University, Gimhae 50834, Republic of KoreaDivision of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USADivision of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USADivision of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USAOur whole-genome sequencing analysis of sixteen uropathogenic <i>E. coli</i> isolates revealed a concerning picture of multidrug resistance and potentially virulent bacteria. All isolates belonged to four distinct clonal groups, with the highly prevalent ST131 lineage being associated with extensive antibiotic resistance and virulence factors. Notably, all isolates exhibited multidrug resistance, with some resistant to as many as 12 antibiotics. Fluoroquinolone resistance stemmed primarily from efflux pumps and mutations in gyrase and topoisomerase genes. Additionally, we identified genes encoding resistance to extended-spectrum cephalosporins, trimethoprim/sulfamethoxazole, and various heavy metals. The presence of diverse plasmids and phages suggests the potential for horizontal gene transfer and the dissemination of virulence factors. All isolates harbored genomic islands containing virulence factors associated with adhesion, biofilm formation, and invasion. Genes essential for iron acquisition, flagella biosynthesis, secretion systems, and toxin production were also prevalent. Adding further complexity to understanding the isolates’ genetic makeup, we identified CRISPR-Cas systems. This study underscores the need for continued genomic surveillance in understanding the pathogenic mechanisms and resistance profiles of uropathogenic <i>E. coli</i> to aid in developing targeted therapeutic strategies.https://www.mdpi.com/2076-0817/13/9/794whole-genome sequencinguropathogen<i>E. coli</i> |
| spellingShingle | Kidon Sung Mohamed Nawaz Miseon Park Jungwhan Chon Saeed A. Khan Khulud Alotaibi Ashraf A. Khan Comprehensive Genomic Analysis of Uropathogenic <i>E. coli</i>: Virulence Factors, Antimicrobial Resistance, and Mobile Genetic Elements Pathogens whole-genome sequencing uropathogen <i>E. coli</i> |
| title | Comprehensive Genomic Analysis of Uropathogenic <i>E. coli</i>: Virulence Factors, Antimicrobial Resistance, and Mobile Genetic Elements |
| title_full | Comprehensive Genomic Analysis of Uropathogenic <i>E. coli</i>: Virulence Factors, Antimicrobial Resistance, and Mobile Genetic Elements |
| title_fullStr | Comprehensive Genomic Analysis of Uropathogenic <i>E. coli</i>: Virulence Factors, Antimicrobial Resistance, and Mobile Genetic Elements |
| title_full_unstemmed | Comprehensive Genomic Analysis of Uropathogenic <i>E. coli</i>: Virulence Factors, Antimicrobial Resistance, and Mobile Genetic Elements |
| title_short | Comprehensive Genomic Analysis of Uropathogenic <i>E. coli</i>: Virulence Factors, Antimicrobial Resistance, and Mobile Genetic Elements |
| title_sort | comprehensive genomic analysis of uropathogenic i e coli i virulence factors antimicrobial resistance and mobile genetic elements |
| topic | whole-genome sequencing uropathogen <i>E. coli</i> |
| url | https://www.mdpi.com/2076-0817/13/9/794 |
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