Phenotypic Profiling of Selected Cellulolytic Strains to Develop a Crop Residue-Decomposing Bacterial Consortium
Slow decomposition rates of cereal crop residues can lead to agronomic challenges, such as nutrient immobilization, delayed soil warming, and increased pest pressures. In this regard, microbial inoculation with efficient strains offers a viable and eco-friendly solution to accelerating the decomposi...
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2025-01-01
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| author | Arman Shamshitov Egidija Satkevičiūtė Francesca Decorosi Carlo Viti Skaidrė Supronienė |
| author_facet | Arman Shamshitov Egidija Satkevičiūtė Francesca Decorosi Carlo Viti Skaidrė Supronienė |
| author_sort | Arman Shamshitov |
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| description | Slow decomposition rates of cereal crop residues can lead to agronomic challenges, such as nutrient immobilization, delayed soil warming, and increased pest pressures. In this regard, microbial inoculation with efficient strains offers a viable and eco-friendly solution to accelerating the decomposition process of crop residues. However, this solution often focuses mostly on selecting microorganisms based on the appropriate enzymic capabilities and neglects the metabolic versatility required to utilize both structural and non-structural components of residues. Therefore, this study aimed to address these limitations by assessing the metabolic profiles of five previously identified cellulolytic bacterial strains, including <i>Bacillus pumilus</i> 1G17, <i>Micromonospora chalcea</i> 1G49, <i>Bacillus mobilis</i> 5G17, <i>Streptomyces canus</i> 1TG5, and <i>Streptomyces achromogenes</i> 3TG21 using Biolog Phenotype Microarray analysis. Moreover, this study evaluated the impact of wheat straw inoculation with single strains and a bacterial consortium on soil organic carbon and nitrogen content in a pot experiment. Results revealed that, beyond the core subset of 12 carbon sources, the strains exhibited diverse metabolic capacities in utilizing 106 carbon sources. All strains demonstrated effective straw biomass degradation compared to the negative control, with significant differences detected only in oil seed rape straw biodegradation estimations. Furthermore, wheat straw inoculated with a bacterial consortium showed a significant increase in soil organic carbon content after 180 days in the pot experiment. Overall, these findings underscore the critical role of metabolic profiling in gaining a deeper understanding of microbial capabilities and addressing the complexities of residue composition and environmental variability. |
| format | Article |
| id | doaj-art-96e3c8655ed045e9adccb97101fda1c5 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-96e3c8655ed045e9adccb97101fda1c52025-01-24T13:43:00ZengMDPI AGMicroorganisms2076-26072025-01-0113119310.3390/microorganisms13010193Phenotypic Profiling of Selected Cellulolytic Strains to Develop a Crop Residue-Decomposing Bacterial ConsortiumArman Shamshitov0Egidija Satkevičiūtė1Francesca Decorosi2Carlo Viti3Skaidrė Supronienė4Microbiology Laboratory, Lithuanian Research Centre for Agriculture and Forestry, Institute of Agriculture, Instituto al. 1, Akademija, LT-58344 Kedainiai, LithuaniaMicrobiology Laboratory, Lithuanian Research Centre for Agriculture and Forestry, Institute of Agriculture, Instituto al. 1, Akademija, LT-58344 Kedainiai, LithuaniaGenexpress Laboratory, Department of Agronomy, Food, Environmental and Forestry (DAGRI), University of Florence, Via della Lastruccia 14, I-50019 Sesto Fiorentino, ItalyGenexpress Laboratory, Department of Agronomy, Food, Environmental and Forestry (DAGRI), University of Florence, Via della Lastruccia 14, I-50019 Sesto Fiorentino, ItalyMicrobiology Laboratory, Lithuanian Research Centre for Agriculture and Forestry, Institute of Agriculture, Instituto al. 1, Akademija, LT-58344 Kedainiai, LithuaniaSlow decomposition rates of cereal crop residues can lead to agronomic challenges, such as nutrient immobilization, delayed soil warming, and increased pest pressures. In this regard, microbial inoculation with efficient strains offers a viable and eco-friendly solution to accelerating the decomposition process of crop residues. However, this solution often focuses mostly on selecting microorganisms based on the appropriate enzymic capabilities and neglects the metabolic versatility required to utilize both structural and non-structural components of residues. Therefore, this study aimed to address these limitations by assessing the metabolic profiles of five previously identified cellulolytic bacterial strains, including <i>Bacillus pumilus</i> 1G17, <i>Micromonospora chalcea</i> 1G49, <i>Bacillus mobilis</i> 5G17, <i>Streptomyces canus</i> 1TG5, and <i>Streptomyces achromogenes</i> 3TG21 using Biolog Phenotype Microarray analysis. Moreover, this study evaluated the impact of wheat straw inoculation with single strains and a bacterial consortium on soil organic carbon and nitrogen content in a pot experiment. Results revealed that, beyond the core subset of 12 carbon sources, the strains exhibited diverse metabolic capacities in utilizing 106 carbon sources. All strains demonstrated effective straw biomass degradation compared to the negative control, with significant differences detected only in oil seed rape straw biodegradation estimations. Furthermore, wheat straw inoculated with a bacterial consortium showed a significant increase in soil organic carbon content after 180 days in the pot experiment. Overall, these findings underscore the critical role of metabolic profiling in gaining a deeper understanding of microbial capabilities and addressing the complexities of residue composition and environmental variability.https://www.mdpi.com/2076-2607/13/1/193cellulolytic bacteriastrawdecompositionphenotype microarrayconsortium |
| spellingShingle | Arman Shamshitov Egidija Satkevičiūtė Francesca Decorosi Carlo Viti Skaidrė Supronienė Phenotypic Profiling of Selected Cellulolytic Strains to Develop a Crop Residue-Decomposing Bacterial Consortium Microorganisms cellulolytic bacteria straw decomposition phenotype microarray consortium |
| title | Phenotypic Profiling of Selected Cellulolytic Strains to Develop a Crop Residue-Decomposing Bacterial Consortium |
| title_full | Phenotypic Profiling of Selected Cellulolytic Strains to Develop a Crop Residue-Decomposing Bacterial Consortium |
| title_fullStr | Phenotypic Profiling of Selected Cellulolytic Strains to Develop a Crop Residue-Decomposing Bacterial Consortium |
| title_full_unstemmed | Phenotypic Profiling of Selected Cellulolytic Strains to Develop a Crop Residue-Decomposing Bacterial Consortium |
| title_short | Phenotypic Profiling of Selected Cellulolytic Strains to Develop a Crop Residue-Decomposing Bacterial Consortium |
| title_sort | phenotypic profiling of selected cellulolytic strains to develop a crop residue decomposing bacterial consortium |
| topic | cellulolytic bacteria straw decomposition phenotype microarray consortium |
| url | https://www.mdpi.com/2076-2607/13/1/193 |
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