Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs
Purpose. To analyze the changes of refraction and metabolism of the retinal cones under monochromatic lights in guinea pigs. Methods. Sixty guinea pigs were randomly divided into a short-wavelength light (SL) group, a middle-wavelength light (ML) group, and a white light (WL) group. Refraction and a...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2018/9197631 |
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| author | Leilei Zou Xiaoyu Zhu Rui Liu Fei Ma Manrong Yu Hong Liu Jinhui Dai |
| author_facet | Leilei Zou Xiaoyu Zhu Rui Liu Fei Ma Manrong Yu Hong Liu Jinhui Dai |
| author_sort | Leilei Zou |
| collection | DOAJ |
| description | Purpose. To analyze the changes of refraction and metabolism of the retinal cones under monochromatic lights in guinea pigs. Methods. Sixty guinea pigs were randomly divided into a short-wavelength light (SL) group, a middle-wavelength light (ML) group, and a white light (WL) group. Refraction and axial length were measured before and after 10-week illumination. The densities of S-cones and M-cones were determined by retinal cone immunocytochemistry, and the expressions of S-opsins and M-opsins were determined by real-time PCR and Western blot. Results. After 10-week illumination, the guinea pigs developed relative hyperopia in the SL group and relative myopia in the ML group. Compared with the WL group, the density of S-cones and S-opsins increased while M-cones and M-opsins decreased in the SL group (all, p<0.05); conversely, the density of S-cones and S-opsins decreased while M-cones and M-opsins increased in the ML group (all, p<0.05). Increased S-cones/opsins and decreased M-cones/opsins were induced by short-wavelength lights. Decreased S-cones/opsins and increased M-cones/opsins were induced by middle-wavelength lights. Conclusions. Altered retinal cones/opsins induced by monochromatic lights might be involved in the refractive development in guinea pigs. |
| format | Article |
| id | doaj-art-96de99fd8e934500825cd2d1096365bd |
| institution | OA Journals |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-96de99fd8e934500825cd2d1096365bd2025-08-20T02:09:48ZengWileyJournal of Ophthalmology2090-004X2090-00582018-01-01201810.1155/2018/91976319197631Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea PigsLeilei Zou0Xiaoyu Zhu1Rui Liu2Fei Ma3Manrong Yu4Hong Liu5Jinhui Dai6Department of Ophthalmology, Eye and ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, ChinaDepartment of Ophthalmology, Eye and ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, ChinaDepartment of Ophthalmology, Eye and ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, ChinaDepartment of Ophthalmology, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, Nanjing 210018, ChinaDepartment of Ophthalmology, Eye and ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, ChinaDepartment of Ophthalmology, Eye and ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, ChinaDepartment of Ophthalmology, Eye and ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, ChinaPurpose. To analyze the changes of refraction and metabolism of the retinal cones under monochromatic lights in guinea pigs. Methods. Sixty guinea pigs were randomly divided into a short-wavelength light (SL) group, a middle-wavelength light (ML) group, and a white light (WL) group. Refraction and axial length were measured before and after 10-week illumination. The densities of S-cones and M-cones were determined by retinal cone immunocytochemistry, and the expressions of S-opsins and M-opsins were determined by real-time PCR and Western blot. Results. After 10-week illumination, the guinea pigs developed relative hyperopia in the SL group and relative myopia in the ML group. Compared with the WL group, the density of S-cones and S-opsins increased while M-cones and M-opsins decreased in the SL group (all, p<0.05); conversely, the density of S-cones and S-opsins decreased while M-cones and M-opsins increased in the ML group (all, p<0.05). Increased S-cones/opsins and decreased M-cones/opsins were induced by short-wavelength lights. Decreased S-cones/opsins and increased M-cones/opsins were induced by middle-wavelength lights. Conclusions. Altered retinal cones/opsins induced by monochromatic lights might be involved in the refractive development in guinea pigs.http://dx.doi.org/10.1155/2018/9197631 |
| spellingShingle | Leilei Zou Xiaoyu Zhu Rui Liu Fei Ma Manrong Yu Hong Liu Jinhui Dai Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs Journal of Ophthalmology |
| title | Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs |
| title_full | Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs |
| title_fullStr | Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs |
| title_full_unstemmed | Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs |
| title_short | Effect of Altered Retinal Cones/Opsins on Refractive Development under Monochromatic Lights in Guinea Pigs |
| title_sort | effect of altered retinal cones opsins on refractive development under monochromatic lights in guinea pigs |
| url | http://dx.doi.org/10.1155/2018/9197631 |
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