Innovative Approaches to Combat Duck Viral Hepatitis: Dual-Specific Anti-DHAV-1 and DHAV-3 Yolk Antibodies
<b>Background and Objectives:</b> Duck viral hepatitis (DVH), a highly contagious and acutely fatal avian disease, is characterized by convulsions, acute death, liver swelling, and hemorrhage, leading to substantial losses in the duck industry. However, there is no efficient prevention a...
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MDPI AG
2025-02-01
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| author | Siqi Lei Yuanhe Yang Chengchen Zhao Anguo Liu Pingli He |
| author_facet | Siqi Lei Yuanhe Yang Chengchen Zhao Anguo Liu Pingli He |
| author_sort | Siqi Lei |
| collection | DOAJ |
| description | <b>Background and Objectives:</b> Duck viral hepatitis (DVH), a highly contagious and acutely fatal avian disease, is characterized by convulsions, acute death, liver swelling, and hemorrhage, leading to substantial losses in the duck industry. However, there is no efficient prevention and control method for DHV infection. Duck hepatitis A virus (DHAV) is one of the primary pathogens responsible for DVH. <b>Methods</b>: In this study, we prepared a highly effective anti-DHAV IgY antibody by immunizing Hy-Line Brown laying hens at the peak of egg production. <b>Results and Conclusions</b>: The neutralization index of this antibody was found to be up to 38.90 (DHAV-1 QYD strain) and 141.25 (DHAV-3 GY strain) in vitro. The antibody also exhibited effective prophylactic effects in a model of hepatic inflammation following the viral challenge of ducklings, with a dose of 0.5 mL per duckling (containing 64 mg/mL of IgY) significantly reducing DHAV-related mortality by 66%, providing substantial protection against the infection. Furthermore, it effectively alleviated oxidative damage caused by DHAV in the ducklings. The results of this study indicate that IgY has the potential for treating DHAV infection; it also provides a new way for the treatment of poultry diseases with specific antibodies. |
| format | Article |
| id | doaj-art-96dd35bb964c4f3db80de8509efde765 |
| institution | OA Journals |
| issn | 2076-393X |
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| publishDate | 2025-02-01 |
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| series | Vaccines |
| spelling | doaj-art-96dd35bb964c4f3db80de8509efde7652025-08-20T02:03:31ZengMDPI AGVaccines2076-393X2025-02-0113215410.3390/vaccines13020154Innovative Approaches to Combat Duck Viral Hepatitis: Dual-Specific Anti-DHAV-1 and DHAV-3 Yolk AntibodiesSiqi Lei0Yuanhe Yang1Chengchen Zhao2Anguo Liu3Pingli He4State Key Laboratory of Animal Nutrition and Feeding, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China<b>Background and Objectives:</b> Duck viral hepatitis (DVH), a highly contagious and acutely fatal avian disease, is characterized by convulsions, acute death, liver swelling, and hemorrhage, leading to substantial losses in the duck industry. However, there is no efficient prevention and control method for DHV infection. Duck hepatitis A virus (DHAV) is one of the primary pathogens responsible for DVH. <b>Methods</b>: In this study, we prepared a highly effective anti-DHAV IgY antibody by immunizing Hy-Line Brown laying hens at the peak of egg production. <b>Results and Conclusions</b>: The neutralization index of this antibody was found to be up to 38.90 (DHAV-1 QYD strain) and 141.25 (DHAV-3 GY strain) in vitro. The antibody also exhibited effective prophylactic effects in a model of hepatic inflammation following the viral challenge of ducklings, with a dose of 0.5 mL per duckling (containing 64 mg/mL of IgY) significantly reducing DHAV-related mortality by 66%, providing substantial protection against the infection. Furthermore, it effectively alleviated oxidative damage caused by DHAV in the ducklings. The results of this study indicate that IgY has the potential for treating DHAV infection; it also provides a new way for the treatment of poultry diseases with specific antibodies.https://www.mdpi.com/2076-393X/13/2/154duck viral hepatitisduck hepatitis A virusegg yolk antibodytreatmentantioxidation |
| spellingShingle | Siqi Lei Yuanhe Yang Chengchen Zhao Anguo Liu Pingli He Innovative Approaches to Combat Duck Viral Hepatitis: Dual-Specific Anti-DHAV-1 and DHAV-3 Yolk Antibodies Vaccines duck viral hepatitis duck hepatitis A virus egg yolk antibody treatment antioxidation |
| title | Innovative Approaches to Combat Duck Viral Hepatitis: Dual-Specific Anti-DHAV-1 and DHAV-3 Yolk Antibodies |
| title_full | Innovative Approaches to Combat Duck Viral Hepatitis: Dual-Specific Anti-DHAV-1 and DHAV-3 Yolk Antibodies |
| title_fullStr | Innovative Approaches to Combat Duck Viral Hepatitis: Dual-Specific Anti-DHAV-1 and DHAV-3 Yolk Antibodies |
| title_full_unstemmed | Innovative Approaches to Combat Duck Viral Hepatitis: Dual-Specific Anti-DHAV-1 and DHAV-3 Yolk Antibodies |
| title_short | Innovative Approaches to Combat Duck Viral Hepatitis: Dual-Specific Anti-DHAV-1 and DHAV-3 Yolk Antibodies |
| title_sort | innovative approaches to combat duck viral hepatitis dual specific anti dhav 1 and dhav 3 yolk antibodies |
| topic | duck viral hepatitis duck hepatitis A virus egg yolk antibody treatment antioxidation |
| url | https://www.mdpi.com/2076-393X/13/2/154 |
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