Spice Defense: Resistance, Capsaicin, and Photosynthesis in Diverse Capsicum Genotypes Under Root-Knot Nematode Stress

<i>Meloidogyne enterolobii</i> is an aggressive root-knot nematode that poses a significant threat to global chili (<i>Capsicum</i> spp.) production. This study evaluated the resistance levels, physiological responses, and capsaicin accumulation patterns of diverse Capsicum g...

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Main Authors: Kansiree Jindapunnapat, Pornthip Sroisai, Nichaphat Auangaree, Nawarat Pornsopin, Suchila Techawongstien, Tanyarat Tarinta
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Horticulturae
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Online Access:https://www.mdpi.com/2311-7524/11/6/607
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author Kansiree Jindapunnapat
Pornthip Sroisai
Nichaphat Auangaree
Nawarat Pornsopin
Suchila Techawongstien
Tanyarat Tarinta
author_facet Kansiree Jindapunnapat
Pornthip Sroisai
Nichaphat Auangaree
Nawarat Pornsopin
Suchila Techawongstien
Tanyarat Tarinta
author_sort Kansiree Jindapunnapat
collection DOAJ
description <i>Meloidogyne enterolobii</i> is an aggressive root-knot nematode that poses a significant threat to global chili (<i>Capsicum</i> spp.) production. This study evaluated the resistance levels, physiological responses, and capsaicin accumulation patterns of diverse Capsicum genotypes—including <i>C. annuum</i>, <i>C. chinense</i>, <i>C. frutescens</i>, and <i>C. baccatum</i>—under nematode-infested and non-infested conditions. Resistance was assessed using the gall index (GI), egg per g of root, and reproductive factor (Rf). Among these evaluated parameters, Rf and egg count consistently reflected nematode reproductive success, whereas the GI proved less reliable for resistance classification. Several genotypes—notably from <i>C. chinense</i> and <i>C. frutescens</i>—exhibited strong resistance (Rf < 1), suggesting their potential for nematode-infection cultivar development. Physiological assessments revealed variable photosynthetic responses, with some genotypes showing increased photosynthetic rates of post-infection, indicating potential compensatory mechanisms. In contrast, capsaicin accumulation was influenced by nematode stress and genetic background, indicating their roles in capsaicin biosynthesis. These findings highlight the genotype-specific biochemical and physiological responses of <i>Capsicum</i> species to <i>M. enterolobii</i> infection and underscore the value of integrating physiological, biochemical, and molecular data in breeding programs. Future research should focus on dissecting hormonal signaling pathways and post-infection metabolic shifts to accelerate the development of robust, high-yielding cultivars with durable resistance.
format Article
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institution Kabale University
issn 2311-7524
language English
publishDate 2025-05-01
publisher MDPI AG
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series Horticulturae
spelling doaj-art-96d61cdc3f9a41a3abc09a27afb015e32025-08-20T03:27:32ZengMDPI AGHorticulturae2311-75242025-05-0111660710.3390/horticulturae11060607Spice Defense: Resistance, Capsaicin, and Photosynthesis in Diverse Capsicum Genotypes Under Root-Knot Nematode StressKansiree Jindapunnapat0Pornthip Sroisai1Nichaphat Auangaree2Nawarat Pornsopin3Suchila Techawongstien4Tanyarat Tarinta5Department of Entomology and Plant-Pathology, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Entomology and Plant-Pathology, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Entomology and Plant-Pathology, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Horticulture, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, ThailandPlant Breeding Research Center for Sustainable Agriculture, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Horticulture, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand<i>Meloidogyne enterolobii</i> is an aggressive root-knot nematode that poses a significant threat to global chili (<i>Capsicum</i> spp.) production. This study evaluated the resistance levels, physiological responses, and capsaicin accumulation patterns of diverse Capsicum genotypes—including <i>C. annuum</i>, <i>C. chinense</i>, <i>C. frutescens</i>, and <i>C. baccatum</i>—under nematode-infested and non-infested conditions. Resistance was assessed using the gall index (GI), egg per g of root, and reproductive factor (Rf). Among these evaluated parameters, Rf and egg count consistently reflected nematode reproductive success, whereas the GI proved less reliable for resistance classification. Several genotypes—notably from <i>C. chinense</i> and <i>C. frutescens</i>—exhibited strong resistance (Rf < 1), suggesting their potential for nematode-infection cultivar development. Physiological assessments revealed variable photosynthetic responses, with some genotypes showing increased photosynthetic rates of post-infection, indicating potential compensatory mechanisms. In contrast, capsaicin accumulation was influenced by nematode stress and genetic background, indicating their roles in capsaicin biosynthesis. These findings highlight the genotype-specific biochemical and physiological responses of <i>Capsicum</i> species to <i>M. enterolobii</i> infection and underscore the value of integrating physiological, biochemical, and molecular data in breeding programs. Future research should focus on dissecting hormonal signaling pathways and post-infection metabolic shifts to accelerate the development of robust, high-yielding cultivars with durable resistance.https://www.mdpi.com/2311-7524/11/6/607biotic stressplant–nematode interactionresistant–susceptible varieties<i>Meloidogyne enterolobii</i>capsaicin biosynthesisphotosynthesis
spellingShingle Kansiree Jindapunnapat
Pornthip Sroisai
Nichaphat Auangaree
Nawarat Pornsopin
Suchila Techawongstien
Tanyarat Tarinta
Spice Defense: Resistance, Capsaicin, and Photosynthesis in Diverse Capsicum Genotypes Under Root-Knot Nematode Stress
Horticulturae
biotic stress
plant–nematode interaction
resistant–susceptible varieties
<i>Meloidogyne enterolobii</i>
capsaicin biosynthesis
photosynthesis
title Spice Defense: Resistance, Capsaicin, and Photosynthesis in Diverse Capsicum Genotypes Under Root-Knot Nematode Stress
title_full Spice Defense: Resistance, Capsaicin, and Photosynthesis in Diverse Capsicum Genotypes Under Root-Knot Nematode Stress
title_fullStr Spice Defense: Resistance, Capsaicin, and Photosynthesis in Diverse Capsicum Genotypes Under Root-Knot Nematode Stress
title_full_unstemmed Spice Defense: Resistance, Capsaicin, and Photosynthesis in Diverse Capsicum Genotypes Under Root-Knot Nematode Stress
title_short Spice Defense: Resistance, Capsaicin, and Photosynthesis in Diverse Capsicum Genotypes Under Root-Knot Nematode Stress
title_sort spice defense resistance capsaicin and photosynthesis in diverse capsicum genotypes under root knot nematode stress
topic biotic stress
plant–nematode interaction
resistant–susceptible varieties
<i>Meloidogyne enterolobii</i>
capsaicin biosynthesis
photosynthesis
url https://www.mdpi.com/2311-7524/11/6/607
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