Bxb1 phage recombinase assists genome engineering in Drosophila melanogaster
Rapid and reliable genome modifications provide the basis for detailed in vivo functional analysis of any genomic entity (gene, regulatory DNA, non-coding RNA, etc). With the advent of CRISPR/Cas9 genome editing technology, manipulation of a particular genomic locus has become a routine undertaking...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-01-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/000114494 |
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| _version_ | 1850152473556156416 |
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| author | Roumen Voutev Richard S. Mann |
| author_facet | Roumen Voutev Richard S. Mann |
| author_sort | Roumen Voutev |
| collection | DOAJ |
| description | Rapid and reliable genome modifications provide the basis for detailed in vivo functional analysis of any genomic entity (gene, regulatory DNA, non-coding RNA, etc). With the advent of CRISPR/Cas9 genome editing technology, manipulation of a particular genomic locus has become a routine undertaking in variety of model organisms, including the fruit fly Drosophila melanogaster. To further diversify the available tools for genome engineering, we successfully harnessed the phage recombinase Bxb1 to perform recombinase-mediated cassette exchange (RMCE) in D. melanogaster. We demonstrate that Bxb1 possesses highly efficient recombinase activity and could be used alone or in conjunction with other currently available recombinases for creating platforms for cassette exchange of targeted loci. |
| format | Article |
| id | doaj-art-96d55635dd7b4ae2b23bf0f0ae7701a4 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-96d55635dd7b4ae2b23bf0f0ae7701a42025-08-20T02:25:58ZengTaylor & Francis GroupBioTechniques0736-62051940-98182017-01-01621373810.2144/000114494Bxb1 phage recombinase assists genome engineering in Drosophila melanogasterRoumen Voutev0Richard S. Mann11Departments of Biochemistry and Molecular Biophysics and Systems Biology, Columbia University, New York, NY1Departments of Biochemistry and Molecular Biophysics and Systems Biology, Columbia University, New York, NYRapid and reliable genome modifications provide the basis for detailed in vivo functional analysis of any genomic entity (gene, regulatory DNA, non-coding RNA, etc). With the advent of CRISPR/Cas9 genome editing technology, manipulation of a particular genomic locus has become a routine undertaking in variety of model organisms, including the fruit fly Drosophila melanogaster. To further diversify the available tools for genome engineering, we successfully harnessed the phage recombinase Bxb1 to perform recombinase-mediated cassette exchange (RMCE) in D. melanogaster. We demonstrate that Bxb1 possesses highly efficient recombinase activity and could be used alone or in conjunction with other currently available recombinases for creating platforms for cassette exchange of targeted loci.https://www.future-science.com/doi/10.2144/000114494Bxb1 recombinasecassette exchangegenome editingDrosophila melanogaster |
| spellingShingle | Roumen Voutev Richard S. Mann Bxb1 phage recombinase assists genome engineering in Drosophila melanogaster BioTechniques Bxb1 recombinase cassette exchange genome editing Drosophila melanogaster |
| title | Bxb1 phage recombinase assists genome engineering in Drosophila melanogaster |
| title_full | Bxb1 phage recombinase assists genome engineering in Drosophila melanogaster |
| title_fullStr | Bxb1 phage recombinase assists genome engineering in Drosophila melanogaster |
| title_full_unstemmed | Bxb1 phage recombinase assists genome engineering in Drosophila melanogaster |
| title_short | Bxb1 phage recombinase assists genome engineering in Drosophila melanogaster |
| title_sort | bxb1 phage recombinase assists genome engineering in drosophila melanogaster |
| topic | Bxb1 recombinase cassette exchange genome editing Drosophila melanogaster |
| url | https://www.future-science.com/doi/10.2144/000114494 |
| work_keys_str_mv | AT roumenvoutev bxb1phagerecombinaseassistsgenomeengineeringindrosophilamelanogaster AT richardsmann bxb1phagerecombinaseassistsgenomeengineeringindrosophilamelanogaster |