Multi-enzymatic biomimetic cerium‐based MOFs mediated precision chemodynamic synergistic antibacteria and tissue repair for MRSA-infected wounds
Abstract Antibiotic-resistant pathogens represent a significant global public health challenge, particularly in refractory infections associated with biofilms. Urgent development of innovative, safe, and therapeutically adaptive strategies to combat these resistant biofilms is essential. We present...
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BMC
2025-05-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-025-03349-3 |
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| author | Shiqi Chen Yifan Li Qiang Ma Jiayi Liang Zhiyue Feng Sihan Wang Shuai Zhang Ke Han Boyan Sun Hongping Wang Haiyang Jiang |
| author_facet | Shiqi Chen Yifan Li Qiang Ma Jiayi Liang Zhiyue Feng Sihan Wang Shuai Zhang Ke Han Boyan Sun Hongping Wang Haiyang Jiang |
| author_sort | Shiqi Chen |
| collection | DOAJ |
| description | Abstract Antibiotic-resistant pathogens represent a significant global public health challenge, particularly in refractory infections associated with biofilms. Urgent development of innovative, safe, and therapeutically adaptive strategies to combat these resistant biofilms is essential. We present a novel biomimetic antibacterial system inspired by the multifunctional enzymatic properties of cerium-based metal–organic frameworks. This system utilizes the inherent oxidase and peroxidase activities of a nanozyme to generate reactive oxygen species (ROS) for bacterial eradication, while its phosphate-ester hydrolase activity disrupts bacterial genetic material and energy metabolism. By the reversible covalent binding between boronic acid groups and cis-diol groups on bacterial surfaces, combined with abundant cerium catalytic sites from the porous structure and the potent antibacterial effects of sanguinarine, we enhance targeted antibacterial activity. This system effectively penetrates extracellular polymeric substances (EPS) and demonstrates precise regulation of ROS, allowing for localized delivery of ROS and sanguinarine for biofilm eradication. Transcriptomic analyses indicate that this approach disrupts the cellular environment, impairs energy metabolism, inhibits bacterial attachment to EPS, and promotes biofilm dispersion by modulating drug resistance-related genes. In vivo experiments confirm that this nanocatalyst composite effectively treats biofilm-induced wounds with efficacy comparable to vancomycin, presenting a promising solution for managing chronic infections caused by antibiotic-resistant biofilms. Graphical Abstract |
| format | Article |
| id | doaj-art-96c920d547144b44ad2836dcd7b964e4 |
| institution | DOAJ |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-96c920d547144b44ad2836dcd7b964e42025-08-20T03:05:59ZengBMCJournal of Nanobiotechnology1477-31552025-05-0123112610.1186/s12951-025-03349-3Multi-enzymatic biomimetic cerium‐based MOFs mediated precision chemodynamic synergistic antibacteria and tissue repair for MRSA-infected woundsShiqi Chen0Yifan Li1Qiang Ma2Jiayi Liang3Zhiyue Feng4Sihan Wang5Shuai Zhang6Ke Han7Boyan Sun8Hongping Wang9Haiyang Jiang10Department of Veterinary Pharmacology and Toxicology, National Key Laboratory of Veterinary Public Health Security, China Agricultural UniversityNMPA Key Laboratory for Quality Control and Evaluation of Vaccines and Biological Products, SiChuan Institute of Musk Deer Breeding, SiChuan Institute for Drug Control (Sichuan Testing Center of Medical Devices)Department of Veterinary Pharmacology and Toxicology, National Key Laboratory of Veterinary Public Health Security, China Agricultural UniversityDepartment of Chemistry, Waterloo Institute for Nanotechnology, University of WaterlooDepartment of Veterinary Pharmacology and Toxicology, National Key Laboratory of Veterinary Public Health Security, China Agricultural UniversityDepartment of Veterinary Pharmacology and Toxicology, National Key Laboratory of Veterinary Public Health Security, China Agricultural UniversityDepartment of Veterinary Pharmacology and Toxicology, National Key Laboratory of Veterinary Public Health Security, China Agricultural UniversityDepartment of Veterinary Pharmacology and Toxicology, National Key Laboratory of Veterinary Public Health Security, China Agricultural UniversityDepartment of Veterinary Pharmacology and Toxicology, National Key Laboratory of Veterinary Public Health Security, China Agricultural UniversityNMPA Key Laboratory for Quality Control and Evaluation of Vaccines and Biological Products, SiChuan Institute of Musk Deer Breeding, SiChuan Institute for Drug Control (Sichuan Testing Center of Medical Devices)Department of Veterinary Pharmacology and Toxicology, National Key Laboratory of Veterinary Public Health Security, China Agricultural UniversityAbstract Antibiotic-resistant pathogens represent a significant global public health challenge, particularly in refractory infections associated with biofilms. Urgent development of innovative, safe, and therapeutically adaptive strategies to combat these resistant biofilms is essential. We present a novel biomimetic antibacterial system inspired by the multifunctional enzymatic properties of cerium-based metal–organic frameworks. This system utilizes the inherent oxidase and peroxidase activities of a nanozyme to generate reactive oxygen species (ROS) for bacterial eradication, while its phosphate-ester hydrolase activity disrupts bacterial genetic material and energy metabolism. By the reversible covalent binding between boronic acid groups and cis-diol groups on bacterial surfaces, combined with abundant cerium catalytic sites from the porous structure and the potent antibacterial effects of sanguinarine, we enhance targeted antibacterial activity. This system effectively penetrates extracellular polymeric substances (EPS) and demonstrates precise regulation of ROS, allowing for localized delivery of ROS and sanguinarine for biofilm eradication. Transcriptomic analyses indicate that this approach disrupts the cellular environment, impairs energy metabolism, inhibits bacterial attachment to EPS, and promotes biofilm dispersion by modulating drug resistance-related genes. In vivo experiments confirm that this nanocatalyst composite effectively treats biofilm-induced wounds with efficacy comparable to vancomycin, presenting a promising solution for managing chronic infections caused by antibiotic-resistant biofilms. Graphical Abstracthttps://doi.org/10.1186/s12951-025-03349-3AntibacteriaNanozymesTargeted delivery systemsMRSAInfected wound healing |
| spellingShingle | Shiqi Chen Yifan Li Qiang Ma Jiayi Liang Zhiyue Feng Sihan Wang Shuai Zhang Ke Han Boyan Sun Hongping Wang Haiyang Jiang Multi-enzymatic biomimetic cerium‐based MOFs mediated precision chemodynamic synergistic antibacteria and tissue repair for MRSA-infected wounds Journal of Nanobiotechnology Antibacteria Nanozymes Targeted delivery systems MRSA Infected wound healing |
| title | Multi-enzymatic biomimetic cerium‐based MOFs mediated precision chemodynamic synergistic antibacteria and tissue repair for MRSA-infected wounds |
| title_full | Multi-enzymatic biomimetic cerium‐based MOFs mediated precision chemodynamic synergistic antibacteria and tissue repair for MRSA-infected wounds |
| title_fullStr | Multi-enzymatic biomimetic cerium‐based MOFs mediated precision chemodynamic synergistic antibacteria and tissue repair for MRSA-infected wounds |
| title_full_unstemmed | Multi-enzymatic biomimetic cerium‐based MOFs mediated precision chemodynamic synergistic antibacteria and tissue repair for MRSA-infected wounds |
| title_short | Multi-enzymatic biomimetic cerium‐based MOFs mediated precision chemodynamic synergistic antibacteria and tissue repair for MRSA-infected wounds |
| title_sort | multi enzymatic biomimetic cerium based mofs mediated precision chemodynamic synergistic antibacteria and tissue repair for mrsa infected wounds |
| topic | Antibacteria Nanozymes Targeted delivery systems MRSA Infected wound healing |
| url | https://doi.org/10.1186/s12951-025-03349-3 |
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