Effect of Storage Time and Temperature on Important Biochemistry Parameters in Stored Human Blood Samples: A Cross-sectional Study
Introduction: Backbone of modern healthcare depends on accuracy of laboratory test results. Sometimes in busy laboratories, samples may have to be stored or transported at different temperatures and for different time periods before they could be analysed. In particularly such samples, validation of...
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JCDR Research and Publications Private Limited
2025-06-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| author | Tajveer Singh Kanda Heena Singla Rajinderjit Singh Ahi |
| author_facet | Tajveer Singh Kanda Heena Singla Rajinderjit Singh Ahi |
| author_sort | Tajveer Singh Kanda |
| collection | DOAJ |
| description | Introduction: Backbone of modern healthcare depends on accuracy of laboratory test results. Sometimes in busy laboratories, samples may have to be stored or transported at different temperatures and for different time periods before they could be analysed. In particularly such samples, validation of test results is very important.
Aim: To study the effect of storage time and temperature on important biochemistry analytes.
Materials and Methods: It was cross-sectional study, carried out in Department of Biochemistry at GGS Medical College, Faridkot, Punjab, India from January 2023 to December 2023. Total 40 patients’ samples were first analysed immediately (0-hour samples) for important biochemical parameters i.e., glucose, urea, creatinine, uric acid, total bilirubin, direct bilirubin, Aspartate Transaminase (AST), Alanine Transaminase (ALT), Alkaline Phosphatase (ALP), sodium, potassium, chloride, amylase, triglycerides, cholesterol, calcium, phosphorus, Creatine Kinase Myoglobin-Binding fraction (CK-MB), Lactate Dehydrogenase (LDH) and N-Acetyl Cysteine Activated Creatine Kinase (CK-NAC). Then samples were stored in two separate aliquots, to be stored at 2-8°C and at room temperature for analysis after 24-hour and 72-hour. Statistical analysis was done with Statistical Package for Social Sciences (SPSS) version 22 software. If p-value was less than 0.05, difference was considered statistically significant.
Results: At 2-8°C, mean values of blood glucose, direct bilirubin, amylase and CK-MB showed statistically highly significant decrease in 24-hour samples, and their mean values were further lower in case of 72-hour samples (all p-values <0.05). Statistically significant increase was noted in mean values of creatinine, uric acid, potassium and phosphorus in samples stored at 2-8°C for 24-hour, which were further higher in 72-hour samples (all p-values again <0.05). In samples stored at room temperature, similar pattern was seen but the changes were more significant as compared to samples stored at 2-8°C. (All p-values again <0.05). Additionally, in samples stored at room temperature, statistically significant decrease was seen in mean values of ALT, while statistically significant increase was noted in mean values of triglycerides, cholesterol, calcium and LDH, while no change was seen in mean values of potassium (p>0.05).
Conclusion: Patient samples should be tested as soon as possible to obtain the correct values, particularly for these mentioned parameters. |
| format | Article |
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| language | English |
| publishDate | 2025-06-01 |
| publisher | JCDR Research and Publications Private Limited |
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| spelling | doaj-art-96bd0bb7f0fb479da5dcb6296b4000c02025-08-20T02:06:11ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2025-06-01196BC06BC1110.7860/JCDR/2025/75599.21112Effect of Storage Time and Temperature on Important Biochemistry Parameters in Stored Human Blood Samples: A Cross-sectional StudyTajveer Singh Kanda0Heena Singla1Rajinderjit Singh Ahi2Postgraduate Student, Department of Biochemistry, Guru Govind Singh Medical College, Faridkot, Punjab, India.Associate Professor, Department of Biochemistry, Guru Govind Singh Medical College, Faridkot, Punjab, India.Professor and Head, Department of Biochemistry, Guru Govind Singh Medical College, Faridkot, Punjab, India.Introduction: Backbone of modern healthcare depends on accuracy of laboratory test results. Sometimes in busy laboratories, samples may have to be stored or transported at different temperatures and for different time periods before they could be analysed. In particularly such samples, validation of test results is very important. Aim: To study the effect of storage time and temperature on important biochemistry analytes. Materials and Methods: It was cross-sectional study, carried out in Department of Biochemistry at GGS Medical College, Faridkot, Punjab, India from January 2023 to December 2023. Total 40 patients’ samples were first analysed immediately (0-hour samples) for important biochemical parameters i.e., glucose, urea, creatinine, uric acid, total bilirubin, direct bilirubin, Aspartate Transaminase (AST), Alanine Transaminase (ALT), Alkaline Phosphatase (ALP), sodium, potassium, chloride, amylase, triglycerides, cholesterol, calcium, phosphorus, Creatine Kinase Myoglobin-Binding fraction (CK-MB), Lactate Dehydrogenase (LDH) and N-Acetyl Cysteine Activated Creatine Kinase (CK-NAC). Then samples were stored in two separate aliquots, to be stored at 2-8°C and at room temperature for analysis after 24-hour and 72-hour. Statistical analysis was done with Statistical Package for Social Sciences (SPSS) version 22 software. If p-value was less than 0.05, difference was considered statistically significant. Results: At 2-8°C, mean values of blood glucose, direct bilirubin, amylase and CK-MB showed statistically highly significant decrease in 24-hour samples, and their mean values were further lower in case of 72-hour samples (all p-values <0.05). Statistically significant increase was noted in mean values of creatinine, uric acid, potassium and phosphorus in samples stored at 2-8°C for 24-hour, which were further higher in 72-hour samples (all p-values again <0.05). In samples stored at room temperature, similar pattern was seen but the changes were more significant as compared to samples stored at 2-8°C. (All p-values again <0.05). Additionally, in samples stored at room temperature, statistically significant decrease was seen in mean values of ALT, while statistically significant increase was noted in mean values of triglycerides, cholesterol, calcium and LDH, while no change was seen in mean values of potassium (p>0.05). Conclusion: Patient samples should be tested as soon as possible to obtain the correct values, particularly for these mentioned parameters.https://jcdr.net/articles/PDF/21112/75599_CE[Ra1]_F(IS)_PF1(Rf_SS)_PFA(IS)_PB(AB_IS)_PN(IS).pdfanalyte stabilitybiochemical analytesresult validationstorage periodstorage temperature |
| spellingShingle | Tajveer Singh Kanda Heena Singla Rajinderjit Singh Ahi Effect of Storage Time and Temperature on Important Biochemistry Parameters in Stored Human Blood Samples: A Cross-sectional Study Journal of Clinical and Diagnostic Research analyte stability biochemical analytes result validation storage period storage temperature |
| title | Effect of Storage Time and Temperature on Important Biochemistry Parameters in Stored Human Blood Samples: A Cross-sectional Study |
| title_full | Effect of Storage Time and Temperature on Important Biochemistry Parameters in Stored Human Blood Samples: A Cross-sectional Study |
| title_fullStr | Effect of Storage Time and Temperature on Important Biochemistry Parameters in Stored Human Blood Samples: A Cross-sectional Study |
| title_full_unstemmed | Effect of Storage Time and Temperature on Important Biochemistry Parameters in Stored Human Blood Samples: A Cross-sectional Study |
| title_short | Effect of Storage Time and Temperature on Important Biochemistry Parameters in Stored Human Blood Samples: A Cross-sectional Study |
| title_sort | effect of storage time and temperature on important biochemistry parameters in stored human blood samples a cross sectional study |
| topic | analyte stability biochemical analytes result validation storage period storage temperature |
| url | https://jcdr.net/articles/PDF/21112/75599_CE[Ra1]_F(IS)_PF1(Rf_SS)_PFA(IS)_PB(AB_IS)_PN(IS).pdf |
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