Acute kidney disease in mice is associated with early cardiovascular dysfunction

Acute kidney injury (AKI) and chronic kidney disease (CKD) are major health concerns due to their increasing incidence and high mortality. They are interconnected syndromes; AKI without recovery evolves into acute kidney disease (AKD), which can indicate an AKI-to-CKD transition. Both AKI and CKD ar...

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Main Authors: Pauline Caillard, Youssef Bennis, Cédric Boudot, Denis Chatelain, Pierre Rybarczyk, Agnès Boullier, Sabrina Poirot, Dimitri Titeca-Beauport, Sandra Bodeau, Gabriel Choukroun, Saïd Kamel, Isabelle Six, Julien Maizel
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Renal Failure
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Online Access:https://www.tandfonline.com/doi/10.1080/0886022X.2024.2415510
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author Pauline Caillard
Youssef Bennis
Cédric Boudot
Denis Chatelain
Pierre Rybarczyk
Agnès Boullier
Sabrina Poirot
Dimitri Titeca-Beauport
Sandra Bodeau
Gabriel Choukroun
Saïd Kamel
Isabelle Six
Julien Maizel
author_facet Pauline Caillard
Youssef Bennis
Cédric Boudot
Denis Chatelain
Pierre Rybarczyk
Agnès Boullier
Sabrina Poirot
Dimitri Titeca-Beauport
Sandra Bodeau
Gabriel Choukroun
Saïd Kamel
Isabelle Six
Julien Maizel
author_sort Pauline Caillard
collection DOAJ
description Acute kidney injury (AKI) and chronic kidney disease (CKD) are major health concerns due to their increasing incidence and high mortality. They are interconnected syndromes; AKI without recovery evolves into acute kidney disease (AKD), which can indicate an AKI-to-CKD transition. Both AKI and CKD are associated with a risk of long-term cardiovascular complications, but whether vascular and cardiac dysfunctions can occur as early as the AKD period has not been studied extensively. In a mouse model of kidney injury (KI) with non-recovery, we performed vasoreactivity and echocardiography analyses on days 15 (D15) and 45 (D45) after KI. We determined the concentrations of two major gut-derived protein-bound uremic toxins known to induce cardiovascular toxicity—indoxyl sulfate (IS) and para-cresyl sulfate (PCS)—and the levels of inflammation and contraction markers on D7, D15, and D45. Mice with KI showed acute tubular and interstitial kidney lesions on D7 and D15 and chronic glomerulosclerosis on D45. They showed significant impairment of aorta relaxation and systolic-diastolic heart function, both on D15 and D45. Such dysfunction was associated with downregulation of the expression of two contractile proteins, αSMA and SERCA2a, with a more pronounced effect on D15 than on D45. KI was also followed by a rapid increase in IS and PCS serum concentrations and the expression induction of pro-inflammatory cytokines and endothelial adhesion molecules in serum and cardiovascular tissues. Therefore, these results highlight that AKD leads to early cardiac and vascular dysfunctions. How these dysfunctions could be managed to prevent cardiovascular events deserves further study.
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series Renal Failure
spelling doaj-art-96ba26bb60574742a2ef773f4dfa8e7a2025-08-20T03:05:25ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492024-12-0146210.1080/0886022X.2024.2415510Acute kidney disease in mice is associated with early cardiovascular dysfunctionPauline Caillard0Youssef Bennis1Cédric Boudot2Denis Chatelain3Pierre Rybarczyk4Agnès Boullier5Sabrina Poirot6Dimitri Titeca-Beauport7Sandra Bodeau8Gabriel Choukroun9Saïd Kamel10Isabelle Six11Julien Maizel12Department of Nephrology, Dialysis and Transplantation, Amiens Medical Center, Amiens, FranceMP3CV laboratory, UR UPJV 7517, University of Picardy Jules Verne, Amiens, FranceMP3CV laboratory, UR UPJV 7517, University of Picardy Jules Verne, Amiens, FranceDepartment of Anatomopathology, Amiens Medical Center, Amiens, FranceHauts-de-France Anatomopathology Institute (i-PatH), Amiens, FranceMP3CV laboratory, UR UPJV 7517, University of Picardy Jules Verne, Amiens, FranceMP3CV laboratory, UR UPJV 7517, University of Picardy Jules Verne, Amiens, FranceDepartment of Nephrology, Dialysis and Transplantation, Amiens Medical Center, Amiens, FranceMP3CV laboratory, UR UPJV 7517, University of Picardy Jules Verne, Amiens, FranceDepartment of Nephrology, Dialysis and Transplantation, Amiens Medical Center, Amiens, FranceMP3CV laboratory, UR UPJV 7517, University of Picardy Jules Verne, Amiens, FranceMP3CV laboratory, UR UPJV 7517, University of Picardy Jules Verne, Amiens, FranceMP3CV laboratory, UR UPJV 7517, University of Picardy Jules Verne, Amiens, FranceAcute kidney injury (AKI) and chronic kidney disease (CKD) are major health concerns due to their increasing incidence and high mortality. They are interconnected syndromes; AKI without recovery evolves into acute kidney disease (AKD), which can indicate an AKI-to-CKD transition. Both AKI and CKD are associated with a risk of long-term cardiovascular complications, but whether vascular and cardiac dysfunctions can occur as early as the AKD period has not been studied extensively. In a mouse model of kidney injury (KI) with non-recovery, we performed vasoreactivity and echocardiography analyses on days 15 (D15) and 45 (D45) after KI. We determined the concentrations of two major gut-derived protein-bound uremic toxins known to induce cardiovascular toxicity—indoxyl sulfate (IS) and para-cresyl sulfate (PCS)—and the levels of inflammation and contraction markers on D7, D15, and D45. Mice with KI showed acute tubular and interstitial kidney lesions on D7 and D15 and chronic glomerulosclerosis on D45. They showed significant impairment of aorta relaxation and systolic-diastolic heart function, both on D15 and D45. Such dysfunction was associated with downregulation of the expression of two contractile proteins, αSMA and SERCA2a, with a more pronounced effect on D15 than on D45. KI was also followed by a rapid increase in IS and PCS serum concentrations and the expression induction of pro-inflammatory cytokines and endothelial adhesion molecules in serum and cardiovascular tissues. Therefore, these results highlight that AKD leads to early cardiac and vascular dysfunctions. How these dysfunctions could be managed to prevent cardiovascular events deserves further study.https://www.tandfonline.com/doi/10.1080/0886022X.2024.2415510Acute kidney injury-to-chronic kidney disease transitioncardiac dysfunctionpro-inflammatory cytokinesprotein-bound uremic toxinsvascular dysfunction
spellingShingle Pauline Caillard
Youssef Bennis
Cédric Boudot
Denis Chatelain
Pierre Rybarczyk
Agnès Boullier
Sabrina Poirot
Dimitri Titeca-Beauport
Sandra Bodeau
Gabriel Choukroun
Saïd Kamel
Isabelle Six
Julien Maizel
Acute kidney disease in mice is associated with early cardiovascular dysfunction
Renal Failure
Acute kidney injury-to-chronic kidney disease transition
cardiac dysfunction
pro-inflammatory cytokines
protein-bound uremic toxins
vascular dysfunction
title Acute kidney disease in mice is associated with early cardiovascular dysfunction
title_full Acute kidney disease in mice is associated with early cardiovascular dysfunction
title_fullStr Acute kidney disease in mice is associated with early cardiovascular dysfunction
title_full_unstemmed Acute kidney disease in mice is associated with early cardiovascular dysfunction
title_short Acute kidney disease in mice is associated with early cardiovascular dysfunction
title_sort acute kidney disease in mice is associated with early cardiovascular dysfunction
topic Acute kidney injury-to-chronic kidney disease transition
cardiac dysfunction
pro-inflammatory cytokines
protein-bound uremic toxins
vascular dysfunction
url https://www.tandfonline.com/doi/10.1080/0886022X.2024.2415510
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