Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye

This study aimed to investigate the presence of Salmonella spp. in chicken doner samples, both ready for consumption and sold in packages in a modified atmosphere, using classical culture technique and the MALDI-TOF method by determining the antibiotic resistance profile structure through the disc d...

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Main Authors: Atakan Karakaya, Ali Gücükoğlu
Format: Article
Language:English
Published: Hasan Eleroğlu 2023-02-01
Series:Turkish Journal of Agriculture: Food Science and Technology
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Online Access:http://www.agrifoodscience.com/index.php/TURJAF/article/view/5638
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author Atakan Karakaya
Ali Gücükoğlu
author_facet Atakan Karakaya
Ali Gücükoğlu
author_sort Atakan Karakaya
collection DOAJ
description This study aimed to investigate the presence of Salmonella spp. in chicken doner samples, both ready for consumption and sold in packages in a modified atmosphere, using classical culture technique and the MALDI-TOF method by determining the antibiotic resistance profile structure through the disc diffusion method by determining the serotypes of the isolates through mPCR. Between September and November 2019, 150 ready-to-eat chicken doner samples (75 of them ready to consume, the other 75 packed in a modified atmosphere) were analyzed. It was found that 23 of the 150 (15.3%) samples analyzed were contaminated with Salmonella spp. 73 isolates were obtained from 23 positive samples. Of these 73 isolates, 33 were found to be Salmonella spp. positive. In serotyping, all 33 isolates were determined to be S. infantis. In the antibiotic resistance profile, 30 of theisolates were resistant to chloramphenicol, 24 to gentamicin, 21 to ampicillin, 20 to trimethoprim-sulfamethoxazole and ciprofloxacin, 18 to tetracycline and amoxicillin-clavulanic acid, and 13 to cefoxitin. As a result, the isolation of Salmonella spp. from the samples and their resistance to different antibiotics were evaluated as potential sources of problems in the context of foodborne infectious diseases.
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institution Kabale University
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publishDate 2023-02-01
publisher Hasan Eleroğlu
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series Turkish Journal of Agriculture: Food Science and Technology
spelling doaj-art-96b4d0bdc9bc4dda99176bd12ed300012025-08-20T03:37:28ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2023-02-0111228028610.24925/turjaf.v11i2.280-286.56384421Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in TürkiyeAtakan Karakaya0https://orcid.org/0000-0002-2386-005XAli Gücükoğlu1https://orcid.org/0000-0002-8465-7768Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, 55200 SamsunDepartment of Food Hygiene and Technology, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, 55200 Samsun, This study aimed to investigate the presence of Salmonella spp. in chicken doner samples, both ready for consumption and sold in packages in a modified atmosphere, using classical culture technique and the MALDI-TOF method by determining the antibiotic resistance profile structure through the disc diffusion method by determining the serotypes of the isolates through mPCR. Between September and November 2019, 150 ready-to-eat chicken doner samples (75 of them ready to consume, the other 75 packed in a modified atmosphere) were analyzed. It was found that 23 of the 150 (15.3%) samples analyzed were contaminated with Salmonella spp. 73 isolates were obtained from 23 positive samples. Of these 73 isolates, 33 were found to be Salmonella spp. positive. In serotyping, all 33 isolates were determined to be S. infantis. In the antibiotic resistance profile, 30 of theisolates were resistant to chloramphenicol, 24 to gentamicin, 21 to ampicillin, 20 to trimethoprim-sulfamethoxazole and ciprofloxacin, 18 to tetracycline and amoxicillin-clavulanic acid, and 13 to cefoxitin. As a result, the isolation of Salmonella spp. from the samples and their resistance to different antibiotics were evaluated as potential sources of problems in the context of foodborne infectious diseases.http://www.agrifoodscience.com/index.php/TURJAF/article/view/5638chicken donersalmonellaantibiotic resistanceserotypingpcr
spellingShingle Atakan Karakaya
Ali Gücükoğlu
Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye
Turkish Journal of Agriculture: Food Science and Technology
chicken doner
salmonella
antibiotic resistance
serotyping
pcr
title Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye
title_full Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye
title_fullStr Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye
title_full_unstemmed Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye
title_short Serotype Distribution and Antimicrobial Resistance Profile of Salmonella Isolated from Traditional Chicken Doner Kebabs in Türkiye
title_sort serotype distribution and antimicrobial resistance profile of salmonella isolated from traditional chicken doner kebabs in turkiye
topic chicken doner
salmonella
antibiotic resistance
serotyping
pcr
url http://www.agrifoodscience.com/index.php/TURJAF/article/view/5638
work_keys_str_mv AT atakankarakaya serotypedistributionandantimicrobialresistanceprofileofsalmonellaisolatedfromtraditionalchickendonerkebabsinturkiye
AT aligucukoglu serotypedistributionandantimicrobialresistanceprofileofsalmonellaisolatedfromtraditionalchickendonerkebabsinturkiye