Hesperidin facilitating gastrointestinal motility by “Gut-brain axis” and “SCF/C-Kit signaling pathways”
ABSTRACT: Hesperidin shows promising results as a potential feed additive for enhancing gastrointestinal motility in animals. Gastrointestinal function plays a pivotal role in animal growth and the digestibility of dietary nutrients, with gastrointestinal motor function serving as a crucial componen...
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Elsevier
2024-12-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124009696 |
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| author | Yunfei Li Xinying Zhou Yusong Du Mingyuan An Shasha Wan Zewei Sun Qingzhen Zhong |
| author_facet | Yunfei Li Xinying Zhou Yusong Du Mingyuan An Shasha Wan Zewei Sun Qingzhen Zhong |
| author_sort | Yunfei Li |
| collection | DOAJ |
| description | ABSTRACT: Hesperidin shows promising results as a potential feed additive for enhancing gastrointestinal motility in animals. Gastrointestinal function plays a pivotal role in animal growth and the digestibility of dietary nutrients, with gastrointestinal motor function serving as a crucial component. However, limited research has been conducted on the application of hesperidin as a feed additive to promote gastrointestinal motility. The present study aims to assess the efficacy of Hesperidin as a feed additive in promoting gastrointestinal motility and elucidating its underlying mechanism. A total of 200 newly hatched (1-day-old) broilers with similar body weight were randomly allocated into 4 groups as follows: the control group receiving only the basal diet, and the other 3 groups supplemented with 50, 100, and 150 mg of hesperidin per kg of the basal diet, respectively. Each group consisted of 5 replicates with ten broilers per replicate, and the feeding trial lasted for a duration of 21 d. At 21 d of age, a 5% w/v Evans Blue solution in distilled water was utilized to measure intestinal transit rates (ITR). Gastric emptying (GE) was evaluated by administering a phenol red solution at a concentration of 0.05% w/v (1 mL/broiler). Fifteen broilers from each group were euthanized and immediately dissected to obtain gizzard, hypothalamus, duodenum, and jugular blood samples. Jugular blood samples were collected for brain-gut peptide content analysis, while gizzard, hypothalamus, and duodenum samples were used for immunohistochemical analysis. Real-time qPCR was performed on gizzard samples. The results demonstrated a significant improvement in the GE and ITR of broilers in all treatment groups compared to the control group (P < 0.05), particularly in the 100mg/Kg and 150mg/Kg hesperidin group. Incorporation of hesperidin into the broilers' diet significantly enhances serum levels of ghrelin, encompassing serotonin (5-HT), motilin (MTL), cholecystokinin (CCK), and Stem Cell Factor (SCF) as well as substance P (SP) in the gizzard and duodenal tissues while reducing vasoactive intestinal peptide (VIP) levels (P < 0.05). The group administered a dosage of 150mg/Kg exhibited the most pronounced effect.Immunohistochemistry analysis revealed that hesperidin supplementation up-regulated SP protein content and down-regulated VIP protein content in the hypothalamus, gizzard, and duodenum of broilers (P < 0.05), with the most pronounced effect illustrated in the 150mg/Kg hesperidin group. Furthermore, addition of hesperidin to broiler feed resulted in a significant up-regulation of protein expression and gene expression related to SCF and The protein expression of Receptor tyrosine kinase (C-Kit) was significantly upregulated in the 150mg/Kg group, while the gene expression of C-Kit was significantly upregulated in the 50 mg/Kg group (P < 0.05). In conclusion, hesperidin exhibits promising potential as a feed additive for broilers, as its dietary supplementation of hesperidin improves gastrointestinal motility through modulation of both “gut-brain axis” signaling pathways and “SCF/C-Kit signaling pathways” within broiler chicken's digestive system. Notably, basal diet supplemented with 150mg/Kg hesperidin demonstrates superior efficacy. |
| format | Article |
| id | doaj-art-96b3e946ca3d47769b822cfb689b6f03 |
| institution | OA Journals |
| issn | 0032-5791 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | Poultry Science |
| spelling | doaj-art-96b3e946ca3d47769b822cfb689b6f032025-08-20T01:55:42ZengElsevierPoultry Science0032-57912024-12-011031210439010.1016/j.psj.2024.104390Hesperidin facilitating gastrointestinal motility by “Gut-brain axis” and “SCF/C-Kit signaling pathways”Yunfei Li0Xinying Zhou1Yusong Du2Mingyuan An3Shasha Wan4Zewei Sun5Qingzhen Zhong6College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Changchun 130118, China; Jilin Key Laboratory of Animal Nutrition and Feed Sciene, Jilin Agricultural University, Changchun 130118, China; Corresponding author:College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Changchun 130118, China; Jilin Key Laboratory of Animal Nutrition and Feed Sciene, Jilin Agricultural University, Changchun 130118, ChinaABSTRACT: Hesperidin shows promising results as a potential feed additive for enhancing gastrointestinal motility in animals. Gastrointestinal function plays a pivotal role in animal growth and the digestibility of dietary nutrients, with gastrointestinal motor function serving as a crucial component. However, limited research has been conducted on the application of hesperidin as a feed additive to promote gastrointestinal motility. The present study aims to assess the efficacy of Hesperidin as a feed additive in promoting gastrointestinal motility and elucidating its underlying mechanism. A total of 200 newly hatched (1-day-old) broilers with similar body weight were randomly allocated into 4 groups as follows: the control group receiving only the basal diet, and the other 3 groups supplemented with 50, 100, and 150 mg of hesperidin per kg of the basal diet, respectively. Each group consisted of 5 replicates with ten broilers per replicate, and the feeding trial lasted for a duration of 21 d. At 21 d of age, a 5% w/v Evans Blue solution in distilled water was utilized to measure intestinal transit rates (ITR). Gastric emptying (GE) was evaluated by administering a phenol red solution at a concentration of 0.05% w/v (1 mL/broiler). Fifteen broilers from each group were euthanized and immediately dissected to obtain gizzard, hypothalamus, duodenum, and jugular blood samples. Jugular blood samples were collected for brain-gut peptide content analysis, while gizzard, hypothalamus, and duodenum samples were used for immunohistochemical analysis. Real-time qPCR was performed on gizzard samples. The results demonstrated a significant improvement in the GE and ITR of broilers in all treatment groups compared to the control group (P < 0.05), particularly in the 100mg/Kg and 150mg/Kg hesperidin group. Incorporation of hesperidin into the broilers' diet significantly enhances serum levels of ghrelin, encompassing serotonin (5-HT), motilin (MTL), cholecystokinin (CCK), and Stem Cell Factor (SCF) as well as substance P (SP) in the gizzard and duodenal tissues while reducing vasoactive intestinal peptide (VIP) levels (P < 0.05). The group administered a dosage of 150mg/Kg exhibited the most pronounced effect.Immunohistochemistry analysis revealed that hesperidin supplementation up-regulated SP protein content and down-regulated VIP protein content in the hypothalamus, gizzard, and duodenum of broilers (P < 0.05), with the most pronounced effect illustrated in the 150mg/Kg hesperidin group. Furthermore, addition of hesperidin to broiler feed resulted in a significant up-regulation of protein expression and gene expression related to SCF and The protein expression of Receptor tyrosine kinase (C-Kit) was significantly upregulated in the 150mg/Kg group, while the gene expression of C-Kit was significantly upregulated in the 50 mg/Kg group (P < 0.05). In conclusion, hesperidin exhibits promising potential as a feed additive for broilers, as its dietary supplementation of hesperidin improves gastrointestinal motility through modulation of both “gut-brain axis” signaling pathways and “SCF/C-Kit signaling pathways” within broiler chicken's digestive system. Notably, basal diet supplemented with 150mg/Kg hesperidin demonstrates superior efficacy.http://www.sciencedirect.com/science/article/pii/S0032579124009696hesperidinbroilergastrointestinal motilitygut-brain axisSCF/C-Kit signaling pathway |
| spellingShingle | Yunfei Li Xinying Zhou Yusong Du Mingyuan An Shasha Wan Zewei Sun Qingzhen Zhong Hesperidin facilitating gastrointestinal motility by “Gut-brain axis” and “SCF/C-Kit signaling pathways” Poultry Science hesperidin broiler gastrointestinal motility gut-brain axis SCF/C-Kit signaling pathway |
| title | Hesperidin facilitating gastrointestinal motility by “Gut-brain axis” and “SCF/C-Kit signaling pathways” |
| title_full | Hesperidin facilitating gastrointestinal motility by “Gut-brain axis” and “SCF/C-Kit signaling pathways” |
| title_fullStr | Hesperidin facilitating gastrointestinal motility by “Gut-brain axis” and “SCF/C-Kit signaling pathways” |
| title_full_unstemmed | Hesperidin facilitating gastrointestinal motility by “Gut-brain axis” and “SCF/C-Kit signaling pathways” |
| title_short | Hesperidin facilitating gastrointestinal motility by “Gut-brain axis” and “SCF/C-Kit signaling pathways” |
| title_sort | hesperidin facilitating gastrointestinal motility by gut brain axis and scf c kit signaling pathways |
| topic | hesperidin broiler gastrointestinal motility gut-brain axis SCF/C-Kit signaling pathway |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124009696 |
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