HIV-1-neutralizing antibody induced by simian adenovirus- and poxvirus MVA-vectored BG505 native-like envelope trimers.
Rabbits and monkeys immunized with HIV type 1 (HIV-1) native-like BG505 SOSIP.664 (BG505s) glycoprotein trimers are known to induce antibodies that can neutralize the autologous tier-2 virus. Here, we assessed the induction of HIV-1 trimer binding and neutralizing antibody (nAb) titres when BG505s t...
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0181886&type=printable |
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| author | Silvia Capucci Edmund G Wee Torben Schiffner Celia C LaBranche Nicola Borthwick Albert Cupo Jonathan Dodd Hansi Dean Quentin Sattentau David Montefiori Per J Klasse Rogier W Sanders John P Moore Tomáš Hanke |
| author_facet | Silvia Capucci Edmund G Wee Torben Schiffner Celia C LaBranche Nicola Borthwick Albert Cupo Jonathan Dodd Hansi Dean Quentin Sattentau David Montefiori Per J Klasse Rogier W Sanders John P Moore Tomáš Hanke |
| author_sort | Silvia Capucci |
| collection | DOAJ |
| description | Rabbits and monkeys immunized with HIV type 1 (HIV-1) native-like BG505 SOSIP.664 (BG505s) glycoprotein trimers are known to induce antibodies that can neutralize the autologous tier-2 virus. Here, we assessed the induction of HIV-1 trimer binding and neutralizing antibody (nAb) titres when BG505s trimers were also delivered by non-replicating simian (chimpanzee) adenovirus and non-replicating poxvirus modified vaccinia virus Ankara (MVA) vaccine vectors. First, we showed that approximately two-thirds and one-third of the trimers secreted from the ChAdOx1.BG505s (C) and MVA.BG505s (M) vaccine-infected cells, respectively, were cleaved and in a native-like conformation. Rabbits were immunized intramuscularly with these vaccine vectors and in some cases boosted with ISCOMATRIX™-adjuvanted BG505s protein trimer (P), using CCC, MMM, PPP, CPP, MPP and CMP vaccine regimens. We found that the peak trimer-binding antibody and tier-1A and autologous tier-2 nAb responses induced by the CC, CM, PPP, CPP, MPP and CMP regimens were comparable, although only PPP induced autologous tier-2 nAbs in all the immunized animals. Three animals developed weak heterologous tier-2 nAbs. These results demonstrate that ChAdOx1 and MVA vectors are useful delivery modalities for not only T-cell, but also antibody vaccine development. |
| format | Article |
| id | doaj-art-96b2207bc5b24d9496ecdec92ae9f12b |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-96b2207bc5b24d9496ecdec92ae9f12b2025-08-20T02:45:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018188610.1371/journal.pone.0181886HIV-1-neutralizing antibody induced by simian adenovirus- and poxvirus MVA-vectored BG505 native-like envelope trimers.Silvia CapucciEdmund G WeeTorben SchiffnerCelia C LaBrancheNicola BorthwickAlbert CupoJonathan DoddHansi DeanQuentin SattentauDavid MontefioriPer J KlasseRogier W SandersJohn P MooreTomáš HankeRabbits and monkeys immunized with HIV type 1 (HIV-1) native-like BG505 SOSIP.664 (BG505s) glycoprotein trimers are known to induce antibodies that can neutralize the autologous tier-2 virus. Here, we assessed the induction of HIV-1 trimer binding and neutralizing antibody (nAb) titres when BG505s trimers were also delivered by non-replicating simian (chimpanzee) adenovirus and non-replicating poxvirus modified vaccinia virus Ankara (MVA) vaccine vectors. First, we showed that approximately two-thirds and one-third of the trimers secreted from the ChAdOx1.BG505s (C) and MVA.BG505s (M) vaccine-infected cells, respectively, were cleaved and in a native-like conformation. Rabbits were immunized intramuscularly with these vaccine vectors and in some cases boosted with ISCOMATRIX™-adjuvanted BG505s protein trimer (P), using CCC, MMM, PPP, CPP, MPP and CMP vaccine regimens. We found that the peak trimer-binding antibody and tier-1A and autologous tier-2 nAb responses induced by the CC, CM, PPP, CPP, MPP and CMP regimens were comparable, although only PPP induced autologous tier-2 nAbs in all the immunized animals. Three animals developed weak heterologous tier-2 nAbs. These results demonstrate that ChAdOx1 and MVA vectors are useful delivery modalities for not only T-cell, but also antibody vaccine development.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0181886&type=printable |
| spellingShingle | Silvia Capucci Edmund G Wee Torben Schiffner Celia C LaBranche Nicola Borthwick Albert Cupo Jonathan Dodd Hansi Dean Quentin Sattentau David Montefiori Per J Klasse Rogier W Sanders John P Moore Tomáš Hanke HIV-1-neutralizing antibody induced by simian adenovirus- and poxvirus MVA-vectored BG505 native-like envelope trimers. PLoS ONE |
| title | HIV-1-neutralizing antibody induced by simian adenovirus- and poxvirus MVA-vectored BG505 native-like envelope trimers. |
| title_full | HIV-1-neutralizing antibody induced by simian adenovirus- and poxvirus MVA-vectored BG505 native-like envelope trimers. |
| title_fullStr | HIV-1-neutralizing antibody induced by simian adenovirus- and poxvirus MVA-vectored BG505 native-like envelope trimers. |
| title_full_unstemmed | HIV-1-neutralizing antibody induced by simian adenovirus- and poxvirus MVA-vectored BG505 native-like envelope trimers. |
| title_short | HIV-1-neutralizing antibody induced by simian adenovirus- and poxvirus MVA-vectored BG505 native-like envelope trimers. |
| title_sort | hiv 1 neutralizing antibody induced by simian adenovirus and poxvirus mva vectored bg505 native like envelope trimers |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0181886&type=printable |
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