Homotypic cell cannibalism, a cell‐death process regulated by the nuclear protein 1, opposes to metastasis in pancreatic cancer

Abstract Pancreatic adenocarcinoma (PDAC) is an extremely deadly disease for which all treatments available have failed to improve life expectancy significantly. This may be explained by the high metastatic potential of PDAC cells, which results from their dedifferentiation towards a mesenchymal phe...

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Main Authors: Carla E. Cano, María José Sandí, Tewfik Hamidi, Ezequiel L. Calvo, Olivier Turrini, Laurent Bartholin, Céline Loncle, Véronique Secq, Stéphane Garcia, Gwen Lomberk, Guido Kroemer, Raul Urrutia, Juan L. Iovanna
Format: Article
Language:English
Published: Springer Nature 2012-07-01
Series:EMBO Molecular Medicine
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Online Access:https://doi.org/10.1002/emmm.201201255
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author Carla E. Cano
María José Sandí
Tewfik Hamidi
Ezequiel L. Calvo
Olivier Turrini
Laurent Bartholin
Céline Loncle
Véronique Secq
Stéphane Garcia
Gwen Lomberk
Guido Kroemer
Raul Urrutia
Juan L. Iovanna
author_facet Carla E. Cano
María José Sandí
Tewfik Hamidi
Ezequiel L. Calvo
Olivier Turrini
Laurent Bartholin
Céline Loncle
Véronique Secq
Stéphane Garcia
Gwen Lomberk
Guido Kroemer
Raul Urrutia
Juan L. Iovanna
author_sort Carla E. Cano
collection DOAJ
description Abstract Pancreatic adenocarcinoma (PDAC) is an extremely deadly disease for which all treatments available have failed to improve life expectancy significantly. This may be explained by the high metastatic potential of PDAC cells, which results from their dedifferentiation towards a mesenchymal phenotype. Some PDAC present cell‐in‐cell structures whose origin and significance are currently unknown. We show here that cell‐in‐cells form after homotypic cell cannibalism (HoCC). We found PDAC patients whose tumours display HoCC develop less metastasis than those without. In vitro, HoCC was promoted by inactivation of the nuclear protein 1 (Nupr1), and was enhanced by treatment with transforming growth factor β. HoCC ends with death of PDAC cells, consistent with a metastasis suppressor role for this phenomenon. Hence, our data indicates a protective role for HoCC in PDAC and identifies Nupr1 as a molecular regulator of this process.
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spelling doaj-art-96a83be0903c43bd9c05ceaec4178d602025-08-20T02:18:35ZengSpringer NatureEMBO Molecular Medicine1757-46761757-46842012-07-014996497910.1002/emmm.201201255Homotypic cell cannibalism, a cell‐death process regulated by the nuclear protein 1, opposes to metastasis in pancreatic cancerCarla E. Cano0María José Sandí1Tewfik Hamidi2Ezequiel L. Calvo3Olivier Turrini4Laurent Bartholin5Céline Loncle6Véronique Secq7Stéphane Garcia8Gwen Lomberk9Guido Kroemer10Raul Urrutia11Juan L. Iovanna12INSERM U1068, CRCM, Cell StressINSERM U1068, CRCM, Cell StressINSERM U1068, CRCM, Cell StressMolecular Endocrinology and Oncology Research Center, CHUL Research CenterInstitut Paoli‐CalmettesINSERM U1052INSERM U1068, CRCM, Cell StressINSERM U1068, CRCM, Cell StressINSERM U1068, CRCM, Cell StressLaboratory of Epigenetics and Chromatin Dynamics, Gastroenterology Research Unit, Departments of Biochemistry and Molecular Biology, Biophysics, and Medicine, Mayo ClinicINSERM, U848Laboratory of Epigenetics and Chromatin Dynamics, Gastroenterology Research Unit, Departments of Biochemistry and Molecular Biology, Biophysics, and Medicine, Mayo ClinicINSERM U1068, CRCM, Cell StressAbstract Pancreatic adenocarcinoma (PDAC) is an extremely deadly disease for which all treatments available have failed to improve life expectancy significantly. This may be explained by the high metastatic potential of PDAC cells, which results from their dedifferentiation towards a mesenchymal phenotype. Some PDAC present cell‐in‐cell structures whose origin and significance are currently unknown. We show here that cell‐in‐cells form after homotypic cell cannibalism (HoCC). We found PDAC patients whose tumours display HoCC develop less metastasis than those without. In vitro, HoCC was promoted by inactivation of the nuclear protein 1 (Nupr1), and was enhanced by treatment with transforming growth factor β. HoCC ends with death of PDAC cells, consistent with a metastasis suppressor role for this phenomenon. Hence, our data indicates a protective role for HoCC in PDAC and identifies Nupr1 as a molecular regulator of this process.https://doi.org/10.1002/emmm.201201255cell cannibalismmetastasisNupr1pancreatic cancerTGFβ
spellingShingle Carla E. Cano
María José Sandí
Tewfik Hamidi
Ezequiel L. Calvo
Olivier Turrini
Laurent Bartholin
Céline Loncle
Véronique Secq
Stéphane Garcia
Gwen Lomberk
Guido Kroemer
Raul Urrutia
Juan L. Iovanna
Homotypic cell cannibalism, a cell‐death process regulated by the nuclear protein 1, opposes to metastasis in pancreatic cancer
EMBO Molecular Medicine
cell cannibalism
metastasis
Nupr1
pancreatic cancer
TGFβ
title Homotypic cell cannibalism, a cell‐death process regulated by the nuclear protein 1, opposes to metastasis in pancreatic cancer
title_full Homotypic cell cannibalism, a cell‐death process regulated by the nuclear protein 1, opposes to metastasis in pancreatic cancer
title_fullStr Homotypic cell cannibalism, a cell‐death process regulated by the nuclear protein 1, opposes to metastasis in pancreatic cancer
title_full_unstemmed Homotypic cell cannibalism, a cell‐death process regulated by the nuclear protein 1, opposes to metastasis in pancreatic cancer
title_short Homotypic cell cannibalism, a cell‐death process regulated by the nuclear protein 1, opposes to metastasis in pancreatic cancer
title_sort homotypic cell cannibalism a cell death process regulated by the nuclear protein 1 opposes to metastasis in pancreatic cancer
topic cell cannibalism
metastasis
Nupr1
pancreatic cancer
TGFβ
url https://doi.org/10.1002/emmm.201201255
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