Green synthesis and characterization of nitrogen-doped carbon dots for enhanced photocatalytic tylosin degradation in soil

Abstract This study reports one-pot green synthesizing and characterizing nitrogen-doped carbon dots (N-CDs) with green-light-emitting fluorescent features. For the said purpose, citrus-containing pulp-free juice was extracted from the lemon (as a green carbon precursor) and subjected to a carboniza...

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Main Authors: María Fernanda Cervantes-Alcaide, Jumanah Ghannam, Reham M. Aldahasi, Afrah E. Mohammed, Hafiz M. N. Iqbal
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-14411-3
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author María Fernanda Cervantes-Alcaide
Jumanah Ghannam
Reham M. Aldahasi
Afrah E. Mohammed
Hafiz M. N. Iqbal
author_facet María Fernanda Cervantes-Alcaide
Jumanah Ghannam
Reham M. Aldahasi
Afrah E. Mohammed
Hafiz M. N. Iqbal
author_sort María Fernanda Cervantes-Alcaide
collection DOAJ
description Abstract This study reports one-pot green synthesizing and characterizing nitrogen-doped carbon dots (N-CDs) with green-light-emitting fluorescent features. For the said purpose, citrus-containing pulp-free juice was extracted from the lemon (as a green carbon precursor) and subjected to a carbonization process in the presence of urea as a doping agent. In addition, the characteristic-dependent factors, i.e., carbonization temperature (i.e., 160 °C, 210 °C, and 260 °C) and reaction periods (i.e., 2 and 4 h), were also varied to investigate their influence on the synthesis and characteristic attributes of N-CDs. Under UV light at 365 nm, the fabricated N-CDs exhibited strong green light emission up to different extents from light green to bright green range. Overall, the green-light-emitting fluorescent potential of all prepared N-CDs was found to be temperature and time-dependent. The UV–Vis spectral profile revealed characteristic absorption peaks of 288, 333, and 407 nm, confirming the formation of N-CDs. The FTIR profile revealed the functional group entities with evident peaks in 3345 cm−1, 2120 cm−1, and 1636 cm−1 regions corresponding to O–H, C=C, and NO2. The surface topology and size distribution characteristics were studied through SEM, TEM, and Image J analysis. The results showed uniformly dispersed particles with a minimum average diameter of 6.63 ± 0.07 nm. The EDS/EDX analysis results validated the elemental composition of the samples, distinctly indicating that carbon predominates as the major element across all samples. The catalytic breakdown potential of N-CDs was tested against Tylosin removal from contaminated soil samples (identified and quantified through LC–MS/MS analysis). This evaluation revealed a notable 39.2% maximum removal efficacy, positioning them as exceptional candidates for catalyzing environmental remediation and protection.
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spelling doaj-art-96a658dbbaad4ef7b8a395ae283d75562025-08-20T03:05:23ZengNature PortfolioScientific Reports2045-23222025-08-0115111510.1038/s41598-025-14411-3Green synthesis and characterization of nitrogen-doped carbon dots for enhanced photocatalytic tylosin degradation in soilMaría Fernanda Cervantes-Alcaide0Jumanah Ghannam1Reham M. Aldahasi2Afrah E. Mohammed3Hafiz M. N. Iqbal4School of Science and Technology, University of SiegenDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman UniversityDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman UniversityDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman UniversityBiomolecular Innovation Group, Laboratorio de Ciencias Naturales, Facultad de Agronomía, Universidad Autónoma de Nuevo LeónAbstract This study reports one-pot green synthesizing and characterizing nitrogen-doped carbon dots (N-CDs) with green-light-emitting fluorescent features. For the said purpose, citrus-containing pulp-free juice was extracted from the lemon (as a green carbon precursor) and subjected to a carbonization process in the presence of urea as a doping agent. In addition, the characteristic-dependent factors, i.e., carbonization temperature (i.e., 160 °C, 210 °C, and 260 °C) and reaction periods (i.e., 2 and 4 h), were also varied to investigate their influence on the synthesis and characteristic attributes of N-CDs. Under UV light at 365 nm, the fabricated N-CDs exhibited strong green light emission up to different extents from light green to bright green range. Overall, the green-light-emitting fluorescent potential of all prepared N-CDs was found to be temperature and time-dependent. The UV–Vis spectral profile revealed characteristic absorption peaks of 288, 333, and 407 nm, confirming the formation of N-CDs. The FTIR profile revealed the functional group entities with evident peaks in 3345 cm−1, 2120 cm−1, and 1636 cm−1 regions corresponding to O–H, C=C, and NO2. The surface topology and size distribution characteristics were studied through SEM, TEM, and Image J analysis. The results showed uniformly dispersed particles with a minimum average diameter of 6.63 ± 0.07 nm. The EDS/EDX analysis results validated the elemental composition of the samples, distinctly indicating that carbon predominates as the major element across all samples. The catalytic breakdown potential of N-CDs was tested against Tylosin removal from contaminated soil samples (identified and quantified through LC–MS/MS analysis). This evaluation revealed a notable 39.2% maximum removal efficacy, positioning them as exceptional candidates for catalyzing environmental remediation and protection.https://doi.org/10.1038/s41598-025-14411-3Carbon dotsNanofabricationGreen one-pot synthesisBioremediation
spellingShingle María Fernanda Cervantes-Alcaide
Jumanah Ghannam
Reham M. Aldahasi
Afrah E. Mohammed
Hafiz M. N. Iqbal
Green synthesis and characterization of nitrogen-doped carbon dots for enhanced photocatalytic tylosin degradation in soil
Scientific Reports
Carbon dots
Nanofabrication
Green one-pot synthesis
Bioremediation
title Green synthesis and characterization of nitrogen-doped carbon dots for enhanced photocatalytic tylosin degradation in soil
title_full Green synthesis and characterization of nitrogen-doped carbon dots for enhanced photocatalytic tylosin degradation in soil
title_fullStr Green synthesis and characterization of nitrogen-doped carbon dots for enhanced photocatalytic tylosin degradation in soil
title_full_unstemmed Green synthesis and characterization of nitrogen-doped carbon dots for enhanced photocatalytic tylosin degradation in soil
title_short Green synthesis and characterization of nitrogen-doped carbon dots for enhanced photocatalytic tylosin degradation in soil
title_sort green synthesis and characterization of nitrogen doped carbon dots for enhanced photocatalytic tylosin degradation in soil
topic Carbon dots
Nanofabrication
Green one-pot synthesis
Bioremediation
url https://doi.org/10.1038/s41598-025-14411-3
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