Ultrasound combined with FeSO4 facilitated the occurrence of ferroptosis in Vibrio parahaemolyticus
Ultrasound (US) as a sustainable non-thermal sterilization technology that is employed either independently alone or in combination with other processing methods to eliminate food-borne pathogens in the food industry. In the present study, the synergistic effects of US combined with FeSO4 against Vi...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Ultrasonics Sonochemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417724003286 |
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| author | Shurui Peng Lishan Yao Xiaolin Zhu Wei Ge Jiakun Deng Hongbo Li Dan Xu Liangbin Hu Haizhen Mo |
| author_facet | Shurui Peng Lishan Yao Xiaolin Zhu Wei Ge Jiakun Deng Hongbo Li Dan Xu Liangbin Hu Haizhen Mo |
| author_sort | Shurui Peng |
| collection | DOAJ |
| description | Ultrasound (US) as a sustainable non-thermal sterilization technology that is employed either independently alone or in combination with other processing methods to eliminate food-borne pathogens in the food industry. In the present study, the synergistic effects of US combined with FeSO4 against Vibrio parahaemolyticus were investigated. The results demonstrated that the combination of ultrasound and FeSO4 had an excellent bactericidal activity on V. parahaemolyticus. Treatment with US (100 W) and FeSO4 (8 μM) for 15 min could kill more than 99.9 % cells. Furthermore, the observed cell death was identified as classical ferroptosis, characterized by ferroptosis hallmarks including iron-dependent, ROS burst, membrane damage and lipid peroxide accumulation. Addition of ferroptosis inhibitor liproxstatin-1 alleviated the cell death induced by the combination treatment. Transcriptome analysis further revealed that the US-FeSO4 treatment significantly influenced pathways related to fatty acid metabolism, ferroptosis, biofilm formation, RNA degradation, oxidative phosphorylation and other key processes, which likely contributed to the occurrence of ferroptosis. Based on these findings, we speculated that cavitation effect of US promoted the entry of Fe2+, leading to the generation of free radicals primarily responsible for ferroptosis by US-FeSO4. Taken together, this study provides valuable insights into the biological pathway involved in ultrasound sterilization and presents an alternative strategy to eradicate microorganism in food products. |
| format | Article |
| id | doaj-art-96940eaab65848aaaea35db28cdc3700 |
| institution | Kabale University |
| issn | 1350-4177 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj-art-96940eaab65848aaaea35db28cdc37002024-11-22T07:36:38ZengElsevierUltrasonics Sonochemistry1350-41772024-12-01111107080Ultrasound combined with FeSO4 facilitated the occurrence of ferroptosis in Vibrio parahaemolyticusShurui Peng0Lishan Yao1Xiaolin Zhu2Wei Ge3Jiakun Deng4Hongbo Li5Dan Xu6Liangbin Hu7Haizhen Mo8School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaCorresponding authors.; School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaCorresponding authors.; School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaUltrasound (US) as a sustainable non-thermal sterilization technology that is employed either independently alone or in combination with other processing methods to eliminate food-borne pathogens in the food industry. In the present study, the synergistic effects of US combined with FeSO4 against Vibrio parahaemolyticus were investigated. The results demonstrated that the combination of ultrasound and FeSO4 had an excellent bactericidal activity on V. parahaemolyticus. Treatment with US (100 W) and FeSO4 (8 μM) for 15 min could kill more than 99.9 % cells. Furthermore, the observed cell death was identified as classical ferroptosis, characterized by ferroptosis hallmarks including iron-dependent, ROS burst, membrane damage and lipid peroxide accumulation. Addition of ferroptosis inhibitor liproxstatin-1 alleviated the cell death induced by the combination treatment. Transcriptome analysis further revealed that the US-FeSO4 treatment significantly influenced pathways related to fatty acid metabolism, ferroptosis, biofilm formation, RNA degradation, oxidative phosphorylation and other key processes, which likely contributed to the occurrence of ferroptosis. Based on these findings, we speculated that cavitation effect of US promoted the entry of Fe2+, leading to the generation of free radicals primarily responsible for ferroptosis by US-FeSO4. Taken together, this study provides valuable insights into the biological pathway involved in ultrasound sterilization and presents an alternative strategy to eradicate microorganism in food products.http://www.sciencedirect.com/science/article/pii/S1350417724003286UltrasoundVibrio parahaemolyticusFeSO4Synergistic sterilizationFerroptosis |
| spellingShingle | Shurui Peng Lishan Yao Xiaolin Zhu Wei Ge Jiakun Deng Hongbo Li Dan Xu Liangbin Hu Haizhen Mo Ultrasound combined with FeSO4 facilitated the occurrence of ferroptosis in Vibrio parahaemolyticus Ultrasonics Sonochemistry Ultrasound Vibrio parahaemolyticus FeSO4 Synergistic sterilization Ferroptosis |
| title | Ultrasound combined with FeSO4 facilitated the occurrence of ferroptosis in Vibrio parahaemolyticus |
| title_full | Ultrasound combined with FeSO4 facilitated the occurrence of ferroptosis in Vibrio parahaemolyticus |
| title_fullStr | Ultrasound combined with FeSO4 facilitated the occurrence of ferroptosis in Vibrio parahaemolyticus |
| title_full_unstemmed | Ultrasound combined with FeSO4 facilitated the occurrence of ferroptosis in Vibrio parahaemolyticus |
| title_short | Ultrasound combined with FeSO4 facilitated the occurrence of ferroptosis in Vibrio parahaemolyticus |
| title_sort | ultrasound combined with feso4 facilitated the occurrence of ferroptosis in vibrio parahaemolyticus |
| topic | Ultrasound Vibrio parahaemolyticus FeSO4 Synergistic sterilization Ferroptosis |
| url | http://www.sciencedirect.com/science/article/pii/S1350417724003286 |
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