Genetic identification of bud sport strain “11-06-25” from “Sanbenti” grape
Grape is likely to produce bud sports during popularization and spread of good cultivars. Bud sport is one kind of somatic mutations, which occurs in meristematic cells of buds. It is an essential source for breeding new varieties in fruit tree because when bud sports grow into branches, some of the...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2017-01-01
|
| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2016.06.201 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850070165844131840 |
|---|---|
| author | PANG Yujie LI Haiyan ZHU Xiaoheng GAO Fuming YIN Yiming JIA Huijuan |
| author_facet | PANG Yujie LI Haiyan ZHU Xiaoheng GAO Fuming YIN Yiming JIA Huijuan |
| author_sort | PANG Yujie |
| collection | DOAJ |
| description | Grape is likely to produce bud sports during popularization and spread of good cultivars. Bud sport is one kind of somatic mutations, which occurs in meristematic cells of buds. It is an essential source for breeding new varieties in fruit tree because when bud sports grow into branches, some of the characters appear different from the original type. Therefore, classification, identification and discussion on their genetic relationship are significant. We discovered a bud sport on “Sanbenti” and named it as “11-06-25”, the maturity time of which is two weeks earlier than that of “Sanbenti”, and is comparable to that of “Zaoxia Wuhe”(a grape variety derived from a bud sport on “Summer Black”). However, “11-06-25” performs better than “Zaoxia Wuhe” at average spike mass, total soluble solids and total aroma content. It is uncertain whether the genetic materials of “11-06-25” and “Zaoxia Wuhe” are identical. Hence it is necessary to conduct genetic research on “11-06-25” in order to study the genetic relationship among these grape lines, thus providing scientific evidence for bud sports identification and new variety development.Using “Sanbenti”,“11-06-25”,“Zaoxia Wuhe” and “Summer Black” and four reference varieties including two Vitis vinifera L.cv. “Muscat of Red Alexandria” and “Shien” along with two Euro-American hybrids “Shine-Muscat” and “Kyoho” as materials, an analysis of their genetic relationship by SSR (simple sequence repeat) was carried out.35 pairs of SSR primers were screened for PCR amplification. By these primers, a total of 135 robust and reproducible bands were generated, of which 124 were polymorphic and the polymorphism frequency was 91.85%. An unweighted pair-group method with arithmetic mean (UPGMA) dendrogram of eight genotypes using NTSYS-pc 2.10e was constructed.Based on our results, it could be established that “Sanbenti”, “11-06-25”, “Zaoxia Wuhe” and “Summer Black” showed high similarity. According to the parents and bud sports relation, the genetic similarity coefficient between “Sanbenti” and “11-06-25” is the highest. Although “11-06-25” and “Zaoxia Wuhe” are similar in some biological traits, the DNA band patterns of “11-06-25” and “Zaoxia Wuhe” varied in four loci (VrZAG15, VrZAG64, VMC4A1 and VMC9A2.1), suggesting that they are not identical grape lines. Besides, during preliminary observation, “11-06-25” showed better color than that of “Sanbenti”, and it also showed better in fruit quality especially aroma than that of “Zaoxia Wuhe” under the same cultivation condition.In conclusion, “11-06-25” is a new grape line derived from “Sanbenti”. It is very worth popularizing because of its early maturity time and great fruit quality. In addition, we presumed that “Sanbenti” may be a bud sport of “Summer Black”. |
| format | Article |
| id | doaj-art-9690aa4ffc0e4712aee014da25ee88d5 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-9690aa4ffc0e4712aee014da25ee88d52025-08-20T02:47:36ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552017-01-0143738010.3785/j.issn.1008-9209.2016.06.20110089209Genetic identification of bud sport strain “11-06-25” from “Sanbenti” grapePANG YujieLI HaiyanZHU XiaohengGAO FumingYIN YimingJIA HuijuanGrape is likely to produce bud sports during popularization and spread of good cultivars. Bud sport is one kind of somatic mutations, which occurs in meristematic cells of buds. It is an essential source for breeding new varieties in fruit tree because when bud sports grow into branches, some of the characters appear different from the original type. Therefore, classification, identification and discussion on their genetic relationship are significant. We discovered a bud sport on “Sanbenti” and named it as “11-06-25”, the maturity time of which is two weeks earlier than that of “Sanbenti”, and is comparable to that of “Zaoxia Wuhe”(a grape variety derived from a bud sport on “Summer Black”). However, “11-06-25” performs better than “Zaoxia Wuhe” at average spike mass, total soluble solids and total aroma content. It is uncertain whether the genetic materials of “11-06-25” and “Zaoxia Wuhe” are identical. Hence it is necessary to conduct genetic research on “11-06-25” in order to study the genetic relationship among these grape lines, thus providing scientific evidence for bud sports identification and new variety development.Using “Sanbenti”,“11-06-25”,“Zaoxia Wuhe” and “Summer Black” and four reference varieties including two Vitis vinifera L.cv. “Muscat of Red Alexandria” and “Shien” along with two Euro-American hybrids “Shine-Muscat” and “Kyoho” as materials, an analysis of their genetic relationship by SSR (simple sequence repeat) was carried out.35 pairs of SSR primers were screened for PCR amplification. By these primers, a total of 135 robust and reproducible bands were generated, of which 124 were polymorphic and the polymorphism frequency was 91.85%. An unweighted pair-group method with arithmetic mean (UPGMA) dendrogram of eight genotypes using NTSYS-pc 2.10e was constructed.Based on our results, it could be established that “Sanbenti”, “11-06-25”, “Zaoxia Wuhe” and “Summer Black” showed high similarity. According to the parents and bud sports relation, the genetic similarity coefficient between “Sanbenti” and “11-06-25” is the highest. Although “11-06-25” and “Zaoxia Wuhe” are similar in some biological traits, the DNA band patterns of “11-06-25” and “Zaoxia Wuhe” varied in four loci (VrZAG15, VrZAG64, VMC4A1 and VMC9A2.1), suggesting that they are not identical grape lines. Besides, during preliminary observation, “11-06-25” showed better color than that of “Sanbenti”, and it also showed better in fruit quality especially aroma than that of “Zaoxia Wuhe” under the same cultivation condition.In conclusion, “11-06-25” is a new grape line derived from “Sanbenti”. It is very worth popularizing because of its early maturity time and great fruit quality. In addition, we presumed that “Sanbenti” may be a bud sport of “Summer Black”.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2016.06.201“Sanbenti” grapebud sportgenetic identificationsimple sequence repeat (SSR) marker |
| spellingShingle | PANG Yujie LI Haiyan ZHU Xiaoheng GAO Fuming YIN Yiming JIA Huijuan Genetic identification of bud sport strain “11-06-25” from “Sanbenti” grape 浙江大学学报. 农业与生命科学版 “Sanbenti” grape bud sport genetic identification simple sequence repeat (SSR) marker |
| title | Genetic identification of bud sport strain “11-06-25” from “Sanbenti” grape |
| title_full | Genetic identification of bud sport strain “11-06-25” from “Sanbenti” grape |
| title_fullStr | Genetic identification of bud sport strain “11-06-25” from “Sanbenti” grape |
| title_full_unstemmed | Genetic identification of bud sport strain “11-06-25” from “Sanbenti” grape |
| title_short | Genetic identification of bud sport strain “11-06-25” from “Sanbenti” grape |
| title_sort | genetic identification of bud sport strain 11 06 25 from sanbenti grape |
| topic | “Sanbenti” grape bud sport genetic identification simple sequence repeat (SSR) marker |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2016.06.201 |
| work_keys_str_mv | AT pangyujie geneticidentificationofbudsportstrain110625fromsanbentigrape AT lihaiyan geneticidentificationofbudsportstrain110625fromsanbentigrape AT zhuxiaoheng geneticidentificationofbudsportstrain110625fromsanbentigrape AT gaofuming geneticidentificationofbudsportstrain110625fromsanbentigrape AT yinyiming geneticidentificationofbudsportstrain110625fromsanbentigrape AT jiahuijuan geneticidentificationofbudsportstrain110625fromsanbentigrape |