Unraveling atomic complexity from frozen samples

Cryo-electron microscopy (cryo-EM) is a significant driver of recent advances in structural biology. Cryo-EM is comprised of several distinct and complementary methods, which include single particle analysis, cryo-electron tomography, and microcrystal electron diffraction. In this Perspective, we wi...

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Main Authors: Yasmeen N. Ruma, Brent L. Nannenga, Tamir Gonen
Format: Article
Language:English
Published: AIP Publishing LLC and ACA 2025-03-01
Series:Structural Dynamics
Online Access:http://dx.doi.org/10.1063/4.0000303
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author Yasmeen N. Ruma
Brent L. Nannenga
Tamir Gonen
author_facet Yasmeen N. Ruma
Brent L. Nannenga
Tamir Gonen
author_sort Yasmeen N. Ruma
collection DOAJ
description Cryo-electron microscopy (cryo-EM) is a significant driver of recent advances in structural biology. Cryo-EM is comprised of several distinct and complementary methods, which include single particle analysis, cryo-electron tomography, and microcrystal electron diffraction. In this Perspective, we will briefly discuss the different branches of cryo-EM in structural biology and the current challenges in these areas.
format Article
id doaj-art-968c9eeafbaf442c8da15016d7de1e35
institution OA Journals
issn 2329-7778
language English
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publisher AIP Publishing LLC and ACA
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series Structural Dynamics
spelling doaj-art-968c9eeafbaf442c8da15016d7de1e352025-08-20T01:48:15ZengAIP Publishing LLC and ACAStructural Dynamics2329-77782025-03-01122020901020901-810.1063/4.0000303Unraveling atomic complexity from frozen samplesYasmeen N. Ruma0Brent L. Nannenga1Tamir Gonen2Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, California 90095, USAChemical Engineering, School for Engineering of Matter, Transport and Energy, Arizona State University, Tempe, Arizona 85287, USADepartment of Biological Chemistry, University of California, Los Angeles, Los Angeles, California 90095, USACryo-electron microscopy (cryo-EM) is a significant driver of recent advances in structural biology. Cryo-EM is comprised of several distinct and complementary methods, which include single particle analysis, cryo-electron tomography, and microcrystal electron diffraction. In this Perspective, we will briefly discuss the different branches of cryo-EM in structural biology and the current challenges in these areas.http://dx.doi.org/10.1063/4.0000303
spellingShingle Yasmeen N. Ruma
Brent L. Nannenga
Tamir Gonen
Unraveling atomic complexity from frozen samples
Structural Dynamics
title Unraveling atomic complexity from frozen samples
title_full Unraveling atomic complexity from frozen samples
title_fullStr Unraveling atomic complexity from frozen samples
title_full_unstemmed Unraveling atomic complexity from frozen samples
title_short Unraveling atomic complexity from frozen samples
title_sort unraveling atomic complexity from frozen samples
url http://dx.doi.org/10.1063/4.0000303
work_keys_str_mv AT yasmeennruma unravelingatomiccomplexityfromfrozensamples
AT brentlnannenga unravelingatomiccomplexityfromfrozensamples
AT tamirgonen unravelingatomiccomplexityfromfrozensamples