Effects of Non-Collagenous Proteins, TGF-β1, and PDGF-BB on Viability and Proliferation of Dental Pulp Stem Cells
Objectives: The dentin matrix servers as a reservoir of growth factors, sequestered during dentinogenesis. The aim of this study was to assess the viability and proliferation of dental pulp stem cells in the presence of dentin matrix-derived non-collagenous proteins and two growth factors; platelet-...
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| Format: | Article |
| Language: | English |
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Lithuanian University of Health Sciences, Faculty of Odontology
2016-03-01
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| Series: | eJournal of Oral Maxillofacial Research |
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| Online Access: | http://www.ejomr.org/JOMR/archives/2016/1/e4/v7n1e4ht.htm |
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| author | Fahimeh Sadat Tabatabaei Maryam Torshabi |
| author_facet | Fahimeh Sadat Tabatabaei Maryam Torshabi |
| author_sort | Fahimeh Sadat Tabatabaei |
| collection | DOAJ |
| description | Objectives: The dentin matrix servers as a reservoir of growth factors, sequestered during dentinogenesis. The aim of this study was to assess the viability and proliferation of dental pulp stem cells in the presence of dentin matrix-derived non-collagenous proteins and two growth factors; platelet-derived growth factor BB and transforming growth factor beta 1.
Material and Methods: The dental pulp cells were isolated and cultured. The dentin proteins were extracted and purified. The MTT assay was performed for assessment of cell viability and proliferation in the presence of different concentrations of dentin proteins and growth factors during 24 - 72 h post-treatment.
Results: The cells treated with 250 ng/mL dentin proteins had the best viability and proliferation ability in comparison with other concentrations (P < 0.05). The MTT assay demonstrated that cells cultured with 5 ng/mL platelet-derived growth factor BB had the highest viability at each time point as compared to other groups (P < 0.05). However, in presence of platelet-derived growth factor BB alone and in combination with transforming growth factor beta 1 and dentin proteins (10 ng/mL), significant higher viability was seen at all time points (P < 0.05). The least viability and proliferation at each growth factor concentration was seen in cells treated with combination of transforming growth factor beta 1 and dentin proteins at 72 h (P < 0.05).
Conclusions: The results indicated that the triple combination of growth factors and matrix-derived non-collagenous proteins (especially at 10 ng/mL concentration) has mitogenic effect on dental pulp stem cells. |
| format | Article |
| id | doaj-art-968786a622d04977906bb38b61d63e06 |
| institution | DOAJ |
| issn | 2029-283X |
| language | English |
| publishDate | 2016-03-01 |
| publisher | Lithuanian University of Health Sciences, Faculty of Odontology |
| record_format | Article |
| series | eJournal of Oral Maxillofacial Research |
| spelling | doaj-art-968786a622d04977906bb38b61d63e062025-08-20T02:52:27ZengLithuanian University of Health Sciences, Faculty of OdontologyeJournal of Oral Maxillofacial Research2029-283X2016-03-0171e410.5037/jomr.2016.7104Effects of Non-Collagenous Proteins, TGF-β1, and PDGF-BB on Viability and Proliferation of Dental Pulp Stem CellsFahimeh Sadat TabatabaeiMaryam TorshabiObjectives: The dentin matrix servers as a reservoir of growth factors, sequestered during dentinogenesis. The aim of this study was to assess the viability and proliferation of dental pulp stem cells in the presence of dentin matrix-derived non-collagenous proteins and two growth factors; platelet-derived growth factor BB and transforming growth factor beta 1. Material and Methods: The dental pulp cells were isolated and cultured. The dentin proteins were extracted and purified. The MTT assay was performed for assessment of cell viability and proliferation in the presence of different concentrations of dentin proteins and growth factors during 24 - 72 h post-treatment. Results: The cells treated with 250 ng/mL dentin proteins had the best viability and proliferation ability in comparison with other concentrations (P < 0.05). The MTT assay demonstrated that cells cultured with 5 ng/mL platelet-derived growth factor BB had the highest viability at each time point as compared to other groups (P < 0.05). However, in presence of platelet-derived growth factor BB alone and in combination with transforming growth factor beta 1 and dentin proteins (10 ng/mL), significant higher viability was seen at all time points (P < 0.05). The least viability and proliferation at each growth factor concentration was seen in cells treated with combination of transforming growth factor beta 1 and dentin proteins at 72 h (P < 0.05). Conclusions: The results indicated that the triple combination of growth factors and matrix-derived non-collagenous proteins (especially at 10 ng/mL concentration) has mitogenic effect on dental pulp stem cells.http://www.ejomr.org/JOMR/archives/2016/1/e4/v7n1e4ht.htmdental pulpdentin protein (non-collagenous)platelet-derived growth factor bbtransforming growth factor beta |
| spellingShingle | Fahimeh Sadat Tabatabaei Maryam Torshabi Effects of Non-Collagenous Proteins, TGF-β1, and PDGF-BB on Viability and Proliferation of Dental Pulp Stem Cells eJournal of Oral Maxillofacial Research dental pulp dentin protein (non-collagenous) platelet-derived growth factor bb transforming growth factor beta |
| title | Effects of Non-Collagenous Proteins, TGF-β1, and PDGF-BB on Viability and Proliferation of Dental Pulp Stem Cells |
| title_full | Effects of Non-Collagenous Proteins, TGF-β1, and PDGF-BB on Viability and Proliferation of Dental Pulp Stem Cells |
| title_fullStr | Effects of Non-Collagenous Proteins, TGF-β1, and PDGF-BB on Viability and Proliferation of Dental Pulp Stem Cells |
| title_full_unstemmed | Effects of Non-Collagenous Proteins, TGF-β1, and PDGF-BB on Viability and Proliferation of Dental Pulp Stem Cells |
| title_short | Effects of Non-Collagenous Proteins, TGF-β1, and PDGF-BB on Viability and Proliferation of Dental Pulp Stem Cells |
| title_sort | effects of non collagenous proteins tgf β1 and pdgf bb on viability and proliferation of dental pulp stem cells |
| topic | dental pulp dentin protein (non-collagenous) platelet-derived growth factor bb transforming growth factor beta |
| url | http://www.ejomr.org/JOMR/archives/2016/1/e4/v7n1e4ht.htm |
| work_keys_str_mv | AT fahimehsadattabatabaei effectsofnoncollagenousproteinstgfb1andpdgfbbonviabilityandproliferationofdentalpulpstemcells AT maryamtorshabi effectsofnoncollagenousproteinstgfb1andpdgfbbonviabilityandproliferationofdentalpulpstemcells |