Comparative mechanistic study of green-synthesized silver-zinc oxide (Ag-ZnO) and iron–Zinc oxide (Fe-ZnO) bimetallic nanoparticles in antiglycation, antidiabetic, antioxidant, and anticancer activities

Bimetallic nanoparticles (BNPs) have gained immense attention for their diverse biological applications. This study aimed to biosynthesize and characterize Ag-ZnONPs and Fe-ZnONPs and compare their potential as therapeutic agents. The BNPs were synthesized using Mentha asiatica plant extract and cha...

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Main Authors: Sumaira Anjum, Mubashra Inam, Amar Yasser Jassim, Iqra Attique, Maryam Zahra, Fayez Althobaiti, Mohamed Mohamed Soliman, Khalid S. Alotaibi, Shatha B. Albattal, Christophe Hano
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Journal of Taibah University for Science
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Online Access:https://www.tandfonline.com/doi/10.1080/16583655.2025.2515712
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author Sumaira Anjum
Mubashra Inam
Amar Yasser Jassim
Iqra Attique
Maryam Zahra
Fayez Althobaiti
Mohamed Mohamed Soliman
Khalid S. Alotaibi
Shatha B. Albattal
Christophe Hano
author_facet Sumaira Anjum
Mubashra Inam
Amar Yasser Jassim
Iqra Attique
Maryam Zahra
Fayez Althobaiti
Mohamed Mohamed Soliman
Khalid S. Alotaibi
Shatha B. Albattal
Christophe Hano
author_sort Sumaira Anjum
collection DOAJ
description Bimetallic nanoparticles (BNPs) have gained immense attention for their diverse biological applications. This study aimed to biosynthesize and characterize Ag-ZnONPs and Fe-ZnONPs and compare their potential as therapeutic agents. The BNPs were synthesized using Mentha asiatica plant extract and characterized using various techniques such as UV-visible spectroscopy, TEM, EDX spectroscopy, ATR-FTIR spectroscopy, PXRD, DLS, and zeta potential analyses. TEM, EDX, and XRD revealed the structural and morphological properties of crystalline Ag-ZnONPs and Fe-ZnONPs. FTIR analysis investigated the plant phytochemicals responsible for the stabilization and capping of BNPs. Ag-ZnONPs showed higher biocompatibility than Fe-ZnONPs. Fe-ZnONPs exhibited-significantly higher anticancer potential than Ag-ZnONPs as evident by Reactive Oxygen Species/ Nitric Oxide Synthase production, along with cell viability analysis, loss of mitochondrial-membrane potential, enhanced caspase-3 gene-expression, and intensified activity of Caspase-3/7. Collectively, our findings underscore the promising therapeutic-potential of BNPs in cancer treatment and diabetes, setting them as strong candidates for future biomedical applications.
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spelling doaj-art-96853a2f7dc14accb1d83f36d5de69cc2025-08-20T02:32:26ZengTaylor & Francis GroupJournal of Taibah University for Science1658-36552025-12-0119110.1080/16583655.2025.2515712Comparative mechanistic study of green-synthesized silver-zinc oxide (Ag-ZnO) and iron–Zinc oxide (Fe-ZnO) bimetallic nanoparticles in antiglycation, antidiabetic, antioxidant, and anticancer activitiesSumaira Anjum0Mubashra Inam1Amar Yasser Jassim2Iqra Attique3Maryam Zahra4Fayez Althobaiti5Mohamed Mohamed Soliman6Khalid S. Alotaibi7Shatha B. Albattal8Christophe Hano9Department of Biotechnology, Kinnaird College for Women, Lahore, PakistanDepartment of Biotechnology, Kinnaird College for Women, Lahore, PakistanMarine Science Center, University of Basrah, Basrah, IraqDepartment of Biotechnology, Kinnaird College for Women, Lahore, PakistanDepartment of Biotechnology, Kinnaird College for Women, Lahore, PakistanDepartment of Biotechnology, College of Science, Taif University, Taif, Saudi ArabiaClinical Laboratory Sciences Department, Turabah University College, Taif University, Taif, Saudi ArabiaGeneral Science and English Language Department, College of Applied Sciences, AlMaarefa University, Riyadh, Saudi ArabiaGeneral Science and English Language Department, College of Applied Sciences, AlMaarefa University, Riyadh, Saudi ArabiaDepartment of Chemical Biology, Eure & Loir Campus, University of Orleans, FranceBimetallic nanoparticles (BNPs) have gained immense attention for their diverse biological applications. This study aimed to biosynthesize and characterize Ag-ZnONPs and Fe-ZnONPs and compare their potential as therapeutic agents. The BNPs were synthesized using Mentha asiatica plant extract and characterized using various techniques such as UV-visible spectroscopy, TEM, EDX spectroscopy, ATR-FTIR spectroscopy, PXRD, DLS, and zeta potential analyses. TEM, EDX, and XRD revealed the structural and morphological properties of crystalline Ag-ZnONPs and Fe-ZnONPs. FTIR analysis investigated the plant phytochemicals responsible for the stabilization and capping of BNPs. Ag-ZnONPs showed higher biocompatibility than Fe-ZnONPs. Fe-ZnONPs exhibited-significantly higher anticancer potential than Ag-ZnONPs as evident by Reactive Oxygen Species/ Nitric Oxide Synthase production, along with cell viability analysis, loss of mitochondrial-membrane potential, enhanced caspase-3 gene-expression, and intensified activity of Caspase-3/7. Collectively, our findings underscore the promising therapeutic-potential of BNPs in cancer treatment and diabetes, setting them as strong candidates for future biomedical applications.https://www.tandfonline.com/doi/10.1080/16583655.2025.2515712Mentha asiaticabimetallic nanoparticlessilver-zinc oxide and iron-zinc oxideanticancerantidiabetic and antiglycationantioxidant
spellingShingle Sumaira Anjum
Mubashra Inam
Amar Yasser Jassim
Iqra Attique
Maryam Zahra
Fayez Althobaiti
Mohamed Mohamed Soliman
Khalid S. Alotaibi
Shatha B. Albattal
Christophe Hano
Comparative mechanistic study of green-synthesized silver-zinc oxide (Ag-ZnO) and iron–Zinc oxide (Fe-ZnO) bimetallic nanoparticles in antiglycation, antidiabetic, antioxidant, and anticancer activities
Journal of Taibah University for Science
Mentha asiatica
bimetallic nanoparticles
silver-zinc oxide and iron-zinc oxide
anticancer
antidiabetic and antiglycation
antioxidant
title Comparative mechanistic study of green-synthesized silver-zinc oxide (Ag-ZnO) and iron–Zinc oxide (Fe-ZnO) bimetallic nanoparticles in antiglycation, antidiabetic, antioxidant, and anticancer activities
title_full Comparative mechanistic study of green-synthesized silver-zinc oxide (Ag-ZnO) and iron–Zinc oxide (Fe-ZnO) bimetallic nanoparticles in antiglycation, antidiabetic, antioxidant, and anticancer activities
title_fullStr Comparative mechanistic study of green-synthesized silver-zinc oxide (Ag-ZnO) and iron–Zinc oxide (Fe-ZnO) bimetallic nanoparticles in antiglycation, antidiabetic, antioxidant, and anticancer activities
title_full_unstemmed Comparative mechanistic study of green-synthesized silver-zinc oxide (Ag-ZnO) and iron–Zinc oxide (Fe-ZnO) bimetallic nanoparticles in antiglycation, antidiabetic, antioxidant, and anticancer activities
title_short Comparative mechanistic study of green-synthesized silver-zinc oxide (Ag-ZnO) and iron–Zinc oxide (Fe-ZnO) bimetallic nanoparticles in antiglycation, antidiabetic, antioxidant, and anticancer activities
title_sort comparative mechanistic study of green synthesized silver zinc oxide ag zno and iron zinc oxide fe zno bimetallic nanoparticles in antiglycation antidiabetic antioxidant and anticancer activities
topic Mentha asiatica
bimetallic nanoparticles
silver-zinc oxide and iron-zinc oxide
anticancer
antidiabetic and antiglycation
antioxidant
url https://www.tandfonline.com/doi/10.1080/16583655.2025.2515712
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