Protocol for profiling RNA m5C methylation at base resolution using m5C-TAC-seq
Summary: RNA 5-methylcytosine (m5C) is a widespread modification and plays a crucial role in gene expression regulation. Here, we present a protocol for transcriptome-wide m5C methylome profiling at base resolution using bisulfite-free m5C detection strategy enabled by ten-eleven translocation (TET)...
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Main Authors: | Xiaoting Zhang, Liang Lu, Chengqi Yi, Xiaoyu Li |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2025-03-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672500005X |
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