Sxt1, Isolated from a Therapeutic Phage Cocktail, Is a Broader Host Range Relative of the Phage T3
Using <i>Escherichia coli</i> BW25113 as a host, we isolated a novel lytic phage from the commercial poly-specific therapeutic phage cocktail Sextaphage<sup>®</sup> (Microgen, Russia). We provide genetic and phenotypic characterization of the phage and describe its host range...
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2024-12-01
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| Online Access: | https://www.mdpi.com/1999-4915/16/12/1905 |
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| author | Polina Iarema Oksana Kotovskaya Mikhail Skutel Alena Drobiazko Andrei Moiseenko Olga Sokolova Alina Samitova Dmitriy Korostin Konstantin Severinov Artem Isaev |
| author_facet | Polina Iarema Oksana Kotovskaya Mikhail Skutel Alena Drobiazko Andrei Moiseenko Olga Sokolova Alina Samitova Dmitriy Korostin Konstantin Severinov Artem Isaev |
| author_sort | Polina Iarema |
| collection | DOAJ |
| description | Using <i>Escherichia coli</i> BW25113 as a host, we isolated a novel lytic phage from the commercial poly-specific therapeutic phage cocktail Sextaphage<sup>®</sup> (Microgen, Russia). We provide genetic and phenotypic characterization of the phage and describe its host range on the ECOR collection of reference <i>E. coli</i> strains. The phage, hereafter named Sxt1, is a close relative of classical coliphage T3 and belongs to the <i>Teetrevirus</i> genus, yet its internal virion proteins, forming an ejectosome, differ from those of T3. In addition, the Sxt1 lateral tail fiber (LTF) protein clusters with those of the phages from the <i>Berlinvirus</i> genus. A comparison of T7, T3, and Sxt1 LTFs reveals the presence of insertions leading to the elongation of Sxt1 tail fibers, which, together with the difference in the HRDRs (host range-determining regions), might explain the expanded host specificity for the Sxt1. |
| format | Article |
| id | doaj-art-9680b0a4c1fb49aa948e852434c7ee4f |
| institution | DOAJ |
| issn | 1999-4915 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-9680b0a4c1fb49aa948e852434c7ee4f2025-08-20T02:43:23ZengMDPI AGViruses1999-49152024-12-011612190510.3390/v16121905Sxt1, Isolated from a Therapeutic Phage Cocktail, Is a Broader Host Range Relative of the Phage T3Polina Iarema0Oksana Kotovskaya1Mikhail Skutel2Alena Drobiazko3Andrei Moiseenko4Olga Sokolova5Alina Samitova6Dmitriy Korostin7Konstantin Severinov8Artem Isaev9Center for Molecular and Cellular Biology, Moscow 121205, RussiaCenter for Molecular and Cellular Biology, Moscow 121205, RussiaCenter for Molecular and Cellular Biology, Moscow 121205, RussiaCenter for Molecular and Cellular Biology, Moscow 121205, RussiaFaculty of Biology, Lomonosov Moscow State University, Moscow 119991, RussiaFaculty of Biology, Lomonosov Moscow State University, Moscow 119991, RussiaCenter for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, Moscow 117997, RussiaCenter for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, Moscow 117997, RussiaInstitute of Gene Biology Russian Academy of Sciences, Moscow 119334, RussiaCenter for Molecular and Cellular Biology, Moscow 121205, RussiaUsing <i>Escherichia coli</i> BW25113 as a host, we isolated a novel lytic phage from the commercial poly-specific therapeutic phage cocktail Sextaphage<sup>®</sup> (Microgen, Russia). We provide genetic and phenotypic characterization of the phage and describe its host range on the ECOR collection of reference <i>E. coli</i> strains. The phage, hereafter named Sxt1, is a close relative of classical coliphage T3 and belongs to the <i>Teetrevirus</i> genus, yet its internal virion proteins, forming an ejectosome, differ from those of T3. In addition, the Sxt1 lateral tail fiber (LTF) protein clusters with those of the phages from the <i>Berlinvirus</i> genus. A comparison of T7, T3, and Sxt1 LTFs reveals the presence of insertions leading to the elongation of Sxt1 tail fibers, which, together with the difference in the HRDRs (host range-determining regions), might explain the expanded host specificity for the Sxt1.https://www.mdpi.com/1999-4915/16/12/1905bacteriophage T3bacteriophage T7Sextaphagephage therapylateral tail fibersejectosome |
| spellingShingle | Polina Iarema Oksana Kotovskaya Mikhail Skutel Alena Drobiazko Andrei Moiseenko Olga Sokolova Alina Samitova Dmitriy Korostin Konstantin Severinov Artem Isaev Sxt1, Isolated from a Therapeutic Phage Cocktail, Is a Broader Host Range Relative of the Phage T3 Viruses bacteriophage T3 bacteriophage T7 Sextaphage phage therapy lateral tail fibers ejectosome |
| title | Sxt1, Isolated from a Therapeutic Phage Cocktail, Is a Broader Host Range Relative of the Phage T3 |
| title_full | Sxt1, Isolated from a Therapeutic Phage Cocktail, Is a Broader Host Range Relative of the Phage T3 |
| title_fullStr | Sxt1, Isolated from a Therapeutic Phage Cocktail, Is a Broader Host Range Relative of the Phage T3 |
| title_full_unstemmed | Sxt1, Isolated from a Therapeutic Phage Cocktail, Is a Broader Host Range Relative of the Phage T3 |
| title_short | Sxt1, Isolated from a Therapeutic Phage Cocktail, Is a Broader Host Range Relative of the Phage T3 |
| title_sort | sxt1 isolated from a therapeutic phage cocktail is a broader host range relative of the phage t3 |
| topic | bacteriophage T3 bacteriophage T7 Sextaphage phage therapy lateral tail fibers ejectosome |
| url | https://www.mdpi.com/1999-4915/16/12/1905 |
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