Calculating Relative Correction Factors for Quantitative Analysis with HILIC-HPLC-ELSD Method: Eight Fructooligosaccharides of Morinda Officinalis as a Case Study
Objective. Because the response of evaporating light scattering detector (ELSD) being in a nonlinear mode, there is no consensus on the method of calculating its relative correction factors (RCF), which limits the application of the quantitative analysis for multi-components by a single marker (QAMS...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2022/8022473 |
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| author | Lihong Zhou Hui Ni Linlin Zhang Wenyong Wu Tengqian Zhang Qi Su Jing Zhou Huali Long Jinjun Hou Jiyu Gong Wanying Wu |
| author_facet | Lihong Zhou Hui Ni Linlin Zhang Wenyong Wu Tengqian Zhang Qi Su Jing Zhou Huali Long Jinjun Hou Jiyu Gong Wanying Wu |
| author_sort | Lihong Zhou |
| collection | DOAJ |
| description | Objective. Because the response of evaporating light scattering detector (ELSD) being in a nonlinear mode, there is no consensus on the method of calculating its relative correction factors (RCF), which limits the application of the quantitative analysis for multi-components by a single marker (QAMS) with LC-ELSD. Methods. Using eight fructooligosaccharides of Morinda officinalis as a case study, the nystose (GF3) as a single standard was adopted to develop a QAMS method to simultaneously determine the other seven fructooligosaccharides with HILIC-HPLC-ELSD method. Six calculation methods of RCF were investigated to select the most reasonable method. The relative error of content between the QAMS and the external standard method (ESM) obtained from 30 batches of samples was used as an indicator to evaluate the six methods. Finally, a chemometrics analysis was performed to find the differential components among MO and its three processing products. Results. It was first reported that only one calculation method was scientific for calculating RCF for the LC-ELSD method. The RCFs of GF3 to the other seven fructooligosaccharides (GF1–GF8) were obtained as 0.86, 0.91, 0.93, 1.05, 1.15, 1.12, and 1.18, respectively. The QAMS of eight fructooligosaccharides of Morinda officinalis was validated with good linearity (R2 > 0.9998) and accepted the accuracy of 95–105% (RSD < 1.81%) based on nystose. Finally, Morinda officinalis and its three processed products were distinguished and could be differed based on the content of the eight fructooligosaccharides. Conclusion. The scientific calculation method of RCF would be of great significance for developing the QAMS method in Pharmacopoeia when performing the LC-ELSD method. |
| format | Article |
| id | doaj-art-967aca82a07d4eb39375e70dbf1d83e2 |
| institution | Kabale University |
| issn | 2090-8873 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-967aca82a07d4eb39375e70dbf1d83e22025-08-20T03:37:29ZengWileyJournal of Analytical Methods in Chemistry2090-88732022-01-01202210.1155/2022/8022473Calculating Relative Correction Factors for Quantitative Analysis with HILIC-HPLC-ELSD Method: Eight Fructooligosaccharides of Morinda Officinalis as a Case StudyLihong Zhou0Hui Ni1Linlin Zhang2Wenyong Wu3Tengqian Zhang4Qi Su5Jing Zhou6Huali Long7Jinjun Hou8Jiyu Gong9Wanying Wu10College of PharmacyNational Engineering Research Center of TCM Standardization TechnologyNational Engineering Research Center of TCM Standardization TechnologyNational Engineering Research Center of TCM Standardization TechnologyNational Engineering Research Center of TCM Standardization TechnologyNational Engineering Research Center of TCM Standardization TechnologyNational Engineering Research Center of TCM Standardization TechnologyNational Engineering Research Center of TCM Standardization TechnologyNational Engineering Research Center of TCM Standardization TechnologyCollege of PharmacyNational Engineering Research Center of TCM Standardization TechnologyObjective. Because the response of evaporating light scattering detector (ELSD) being in a nonlinear mode, there is no consensus on the method of calculating its relative correction factors (RCF), which limits the application of the quantitative analysis for multi-components by a single marker (QAMS) with LC-ELSD. Methods. Using eight fructooligosaccharides of Morinda officinalis as a case study, the nystose (GF3) as a single standard was adopted to develop a QAMS method to simultaneously determine the other seven fructooligosaccharides with HILIC-HPLC-ELSD method. Six calculation methods of RCF were investigated to select the most reasonable method. The relative error of content between the QAMS and the external standard method (ESM) obtained from 30 batches of samples was used as an indicator to evaluate the six methods. Finally, a chemometrics analysis was performed to find the differential components among MO and its three processing products. Results. It was first reported that only one calculation method was scientific for calculating RCF for the LC-ELSD method. The RCFs of GF3 to the other seven fructooligosaccharides (GF1–GF8) were obtained as 0.86, 0.91, 0.93, 1.05, 1.15, 1.12, and 1.18, respectively. The QAMS of eight fructooligosaccharides of Morinda officinalis was validated with good linearity (R2 > 0.9998) and accepted the accuracy of 95–105% (RSD < 1.81%) based on nystose. Finally, Morinda officinalis and its three processed products were distinguished and could be differed based on the content of the eight fructooligosaccharides. Conclusion. The scientific calculation method of RCF would be of great significance for developing the QAMS method in Pharmacopoeia when performing the LC-ELSD method.http://dx.doi.org/10.1155/2022/8022473 |
| spellingShingle | Lihong Zhou Hui Ni Linlin Zhang Wenyong Wu Tengqian Zhang Qi Su Jing Zhou Huali Long Jinjun Hou Jiyu Gong Wanying Wu Calculating Relative Correction Factors for Quantitative Analysis with HILIC-HPLC-ELSD Method: Eight Fructooligosaccharides of Morinda Officinalis as a Case Study Journal of Analytical Methods in Chemistry |
| title | Calculating Relative Correction Factors for Quantitative Analysis with HILIC-HPLC-ELSD Method: Eight Fructooligosaccharides of Morinda Officinalis as a Case Study |
| title_full | Calculating Relative Correction Factors for Quantitative Analysis with HILIC-HPLC-ELSD Method: Eight Fructooligosaccharides of Morinda Officinalis as a Case Study |
| title_fullStr | Calculating Relative Correction Factors for Quantitative Analysis with HILIC-HPLC-ELSD Method: Eight Fructooligosaccharides of Morinda Officinalis as a Case Study |
| title_full_unstemmed | Calculating Relative Correction Factors for Quantitative Analysis with HILIC-HPLC-ELSD Method: Eight Fructooligosaccharides of Morinda Officinalis as a Case Study |
| title_short | Calculating Relative Correction Factors for Quantitative Analysis with HILIC-HPLC-ELSD Method: Eight Fructooligosaccharides of Morinda Officinalis as a Case Study |
| title_sort | calculating relative correction factors for quantitative analysis with hilic hplc elsd method eight fructooligosaccharides of morinda officinalis as a case study |
| url | http://dx.doi.org/10.1155/2022/8022473 |
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