Evaluation physical characteristics and comparison antimicrobial and anti-inflammation potentials of dental root canal sealers containing hinokitiol in vitro.
Hinokitiol displays potent antimicrobial activity. It has been used in toothpaste and oral-care gel to improve the oral lichen planus and reduce halitosis. The aim of this study was to evaluate the antimicrobial activity of 3 different dental root canal sealers with hinokitiol (sealers+H) and their...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0094941&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850023658068639744 |
|---|---|
| author | Yin-Hua Shih Dan-Jae Lin Kuo-Wei Chang Shih-Min Hsia Shun-Yao Ko Shyh-Yuan Lee Shui-Sang Hsue Tong-Hong Wang Yi-Ling Chen Tzong-Ming Shieh |
| author_facet | Yin-Hua Shih Dan-Jae Lin Kuo-Wei Chang Shih-Min Hsia Shun-Yao Ko Shyh-Yuan Lee Shui-Sang Hsue Tong-Hong Wang Yi-Ling Chen Tzong-Ming Shieh |
| author_sort | Yin-Hua Shih |
| collection | DOAJ |
| description | Hinokitiol displays potent antimicrobial activity. It has been used in toothpaste and oral-care gel to improve the oral lichen planus and reduce halitosis. The aim of this study was to evaluate the antimicrobial activity of 3 different dental root canal sealers with hinokitiol (sealers+H) and their physical and biological effects. AH Plus (epoxy amine resin-based, AH), Apexit Plus (calcium-hydroxide-based, AP), and Canals (zinc-oxide-eugenol-based, CA), were used in this study. The original AH and CA exhibited strong anti-methicillin-resistant Staphylococcus aureus (anti-MRSA) activity, but AP did not. The setting time, working time, flowability, film thickness, and solubility of each sealer+0.2%H complied with ISO 6876:2001. CA+0.2%H exhibited high cytotoxicity, but the others sealers+0.2%H did not. Because hinokitiol combined with Zn2+ in CA creates a synergistic effect. The physical tests of AP+0.5%-1%H complied with ISO 6876:2001, improved antimicrobial activity, inhibited inflammation genes cyclooxygenase-2 (COX-2) and hypoxia-inducible factor-1α (HIF-1α) mRNA in MG-63 cells and human gingival fibroblasts (HGF), and down-regulated lysyl oxidase (LOX) mRNA of HGF. In summary, AH and CA demonstrated strong antimicrobial activity, but AP did not. Application of hinokitiol increases AH anti-MRSA activity should less than 0.2% for keep well flowability. AP+0.5%-1% hinokitiol exhibited strong physical, antibacterial, and anti-inflammation potentials, and inhibited S. aureus abscess formation. Applying an appreciable proportion of hinokitiol to epoxy-amine-resin-based and calcium-hydroxide-based root canal sealers is warranted, but the enhanced cytotoxicity and synergistic effect must be considered. |
| format | Article |
| id | doaj-art-965e7f7b19cc4331978d3b32c7ca1c27 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-965e7f7b19cc4331978d3b32c7ca1c272025-08-20T03:01:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0196e9494110.1371/journal.pone.0094941Evaluation physical characteristics and comparison antimicrobial and anti-inflammation potentials of dental root canal sealers containing hinokitiol in vitro.Yin-Hua ShihDan-Jae LinKuo-Wei ChangShih-Min HsiaShun-Yao KoShyh-Yuan LeeShui-Sang HsueTong-Hong WangYi-Ling ChenTzong-Ming ShiehHinokitiol displays potent antimicrobial activity. It has been used in toothpaste and oral-care gel to improve the oral lichen planus and reduce halitosis. The aim of this study was to evaluate the antimicrobial activity of 3 different dental root canal sealers with hinokitiol (sealers+H) and their physical and biological effects. AH Plus (epoxy amine resin-based, AH), Apexit Plus (calcium-hydroxide-based, AP), and Canals (zinc-oxide-eugenol-based, CA), were used in this study. The original AH and CA exhibited strong anti-methicillin-resistant Staphylococcus aureus (anti-MRSA) activity, but AP did not. The setting time, working time, flowability, film thickness, and solubility of each sealer+0.2%H complied with ISO 6876:2001. CA+0.2%H exhibited high cytotoxicity, but the others sealers+0.2%H did not. Because hinokitiol combined with Zn2+ in CA creates a synergistic effect. The physical tests of AP+0.5%-1%H complied with ISO 6876:2001, improved antimicrobial activity, inhibited inflammation genes cyclooxygenase-2 (COX-2) and hypoxia-inducible factor-1α (HIF-1α) mRNA in MG-63 cells and human gingival fibroblasts (HGF), and down-regulated lysyl oxidase (LOX) mRNA of HGF. In summary, AH and CA demonstrated strong antimicrobial activity, but AP did not. Application of hinokitiol increases AH anti-MRSA activity should less than 0.2% for keep well flowability. AP+0.5%-1% hinokitiol exhibited strong physical, antibacterial, and anti-inflammation potentials, and inhibited S. aureus abscess formation. Applying an appreciable proportion of hinokitiol to epoxy-amine-resin-based and calcium-hydroxide-based root canal sealers is warranted, but the enhanced cytotoxicity and synergistic effect must be considered.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0094941&type=printable |
| spellingShingle | Yin-Hua Shih Dan-Jae Lin Kuo-Wei Chang Shih-Min Hsia Shun-Yao Ko Shyh-Yuan Lee Shui-Sang Hsue Tong-Hong Wang Yi-Ling Chen Tzong-Ming Shieh Evaluation physical characteristics and comparison antimicrobial and anti-inflammation potentials of dental root canal sealers containing hinokitiol in vitro. PLoS ONE |
| title | Evaluation physical characteristics and comparison antimicrobial and anti-inflammation potentials of dental root canal sealers containing hinokitiol in vitro. |
| title_full | Evaluation physical characteristics and comparison antimicrobial and anti-inflammation potentials of dental root canal sealers containing hinokitiol in vitro. |
| title_fullStr | Evaluation physical characteristics and comparison antimicrobial and anti-inflammation potentials of dental root canal sealers containing hinokitiol in vitro. |
| title_full_unstemmed | Evaluation physical characteristics and comparison antimicrobial and anti-inflammation potentials of dental root canal sealers containing hinokitiol in vitro. |
| title_short | Evaluation physical characteristics and comparison antimicrobial and anti-inflammation potentials of dental root canal sealers containing hinokitiol in vitro. |
| title_sort | evaluation physical characteristics and comparison antimicrobial and anti inflammation potentials of dental root canal sealers containing hinokitiol in vitro |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0094941&type=printable |
| work_keys_str_mv | AT yinhuashih evaluationphysicalcharacteristicsandcomparisonantimicrobialandantiinflammationpotentialsofdentalrootcanalsealerscontaininghinokitiolinvitro AT danjaelin evaluationphysicalcharacteristicsandcomparisonantimicrobialandantiinflammationpotentialsofdentalrootcanalsealerscontaininghinokitiolinvitro AT kuoweichang evaluationphysicalcharacteristicsandcomparisonantimicrobialandantiinflammationpotentialsofdentalrootcanalsealerscontaininghinokitiolinvitro AT shihminhsia evaluationphysicalcharacteristicsandcomparisonantimicrobialandantiinflammationpotentialsofdentalrootcanalsealerscontaininghinokitiolinvitro AT shunyaoko evaluationphysicalcharacteristicsandcomparisonantimicrobialandantiinflammationpotentialsofdentalrootcanalsealerscontaininghinokitiolinvitro AT shyhyuanlee evaluationphysicalcharacteristicsandcomparisonantimicrobialandantiinflammationpotentialsofdentalrootcanalsealerscontaininghinokitiolinvitro AT shuisanghsue evaluationphysicalcharacteristicsandcomparisonantimicrobialandantiinflammationpotentialsofdentalrootcanalsealerscontaininghinokitiolinvitro AT tonghongwang evaluationphysicalcharacteristicsandcomparisonantimicrobialandantiinflammationpotentialsofdentalrootcanalsealerscontaininghinokitiolinvitro AT yilingchen evaluationphysicalcharacteristicsandcomparisonantimicrobialandantiinflammationpotentialsofdentalrootcanalsealerscontaininghinokitiolinvitro AT tzongmingshieh evaluationphysicalcharacteristicsandcomparisonantimicrobialandantiinflammationpotentialsofdentalrootcanalsealerscontaininghinokitiolinvitro |