Arabidopsis root apical meristem adaptation to an osmotic gradient condition: an integrated approach from cell expansion to gene expression
Climate change triggers abiotic stress, such as drought and high salinity, that can cause osmotic stress. Water availability can limit plant growth, and the root tip tissues initially sense it. Most experiments destined to understand root growth adaptation to osmotic stress apply homogeneous high os...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-11-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1465219/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850197904692609024 |
|---|---|
| author | Selene Píriz-Pezzutto Mauro Martínez-Moré María Martha Sainz Omar Borsani Mariana Sotelo-Silveira |
| author_facet | Selene Píriz-Pezzutto Mauro Martínez-Moré María Martha Sainz Omar Borsani Mariana Sotelo-Silveira |
| author_sort | Selene Píriz-Pezzutto |
| collection | DOAJ |
| description | Climate change triggers abiotic stress, such as drought and high salinity, that can cause osmotic stress. Water availability can limit plant growth, and the root tip tissues initially sense it. Most experiments destined to understand root growth adaptation to osmotic stress apply homogeneous high osmotic potentials (osmotic shock) to shoots and roots. However, this treatment does not represent natural field conditions where a root may encounter increasing osmotic potentials while exploring the soil. Osmotic shock severely reduces root growth rate, decreasing cell division in the proximal meristem and reducing mature cell length. In this work, we developed an in vitro osmotic gradient experimental system with increasing osmotic potentials. The system generates a controlled osmotic gradient in the root growth zone while exposing the aerial tissues to control conditions. The osmotic gradient system allowed Arabidopsis seedlings of Col-0 and ttl1 mutant (affected in the gene TETRATRICOPEPTIDE THIOREDOXIN-LIKE 1 (TTL1)) to sustain proper root growth for 25 days, reaching osmotic potentials of -1.2 MPa. We demonstrated that roots of seedlings grown in the osmotic gradient sustain a higher root growth rate than those that were grown under a homogeneous high osmotic potential. Furthermore, we found out that the expression of some genes is modified in the roots grown in the osmotic gradient compared to those grown in osmotic shock. Our data indicate that using an osmotic gradient can improve our understanding of how plants respond to osmotic stress and help find new genes to improve plant field performance. |
| format | Article |
| id | doaj-art-9654cc2bfbf34ea8a6b20aaffe9551a6 |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-9654cc2bfbf34ea8a6b20aaffe9551a62025-08-20T02:13:01ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-11-011510.3389/fpls.2024.14652191465219Arabidopsis root apical meristem adaptation to an osmotic gradient condition: an integrated approach from cell expansion to gene expressionSelene Píriz-PezzuttoMauro Martínez-MoréMaría Martha SainzOmar BorsaniMariana Sotelo-SilveiraClimate change triggers abiotic stress, such as drought and high salinity, that can cause osmotic stress. Water availability can limit plant growth, and the root tip tissues initially sense it. Most experiments destined to understand root growth adaptation to osmotic stress apply homogeneous high osmotic potentials (osmotic shock) to shoots and roots. However, this treatment does not represent natural field conditions where a root may encounter increasing osmotic potentials while exploring the soil. Osmotic shock severely reduces root growth rate, decreasing cell division in the proximal meristem and reducing mature cell length. In this work, we developed an in vitro osmotic gradient experimental system with increasing osmotic potentials. The system generates a controlled osmotic gradient in the root growth zone while exposing the aerial tissues to control conditions. The osmotic gradient system allowed Arabidopsis seedlings of Col-0 and ttl1 mutant (affected in the gene TETRATRICOPEPTIDE THIOREDOXIN-LIKE 1 (TTL1)) to sustain proper root growth for 25 days, reaching osmotic potentials of -1.2 MPa. We demonstrated that roots of seedlings grown in the osmotic gradient sustain a higher root growth rate than those that were grown under a homogeneous high osmotic potential. Furthermore, we found out that the expression of some genes is modified in the roots grown in the osmotic gradient compared to those grown in osmotic shock. Our data indicate that using an osmotic gradient can improve our understanding of how plants respond to osmotic stress and help find new genes to improve plant field performance.https://www.frontiersin.org/articles/10.3389/fpls.2024.1465219/fullosmotic stressgradientroot growthArabidopsisTTL1 |
| spellingShingle | Selene Píriz-Pezzutto Mauro Martínez-Moré María Martha Sainz Omar Borsani Mariana Sotelo-Silveira Arabidopsis root apical meristem adaptation to an osmotic gradient condition: an integrated approach from cell expansion to gene expression Frontiers in Plant Science osmotic stress gradient root growth Arabidopsis TTL1 |
| title | Arabidopsis root apical meristem adaptation to an osmotic gradient condition: an integrated approach from cell expansion to gene expression |
| title_full | Arabidopsis root apical meristem adaptation to an osmotic gradient condition: an integrated approach from cell expansion to gene expression |
| title_fullStr | Arabidopsis root apical meristem adaptation to an osmotic gradient condition: an integrated approach from cell expansion to gene expression |
| title_full_unstemmed | Arabidopsis root apical meristem adaptation to an osmotic gradient condition: an integrated approach from cell expansion to gene expression |
| title_short | Arabidopsis root apical meristem adaptation to an osmotic gradient condition: an integrated approach from cell expansion to gene expression |
| title_sort | arabidopsis root apical meristem adaptation to an osmotic gradient condition an integrated approach from cell expansion to gene expression |
| topic | osmotic stress gradient root growth Arabidopsis TTL1 |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1465219/full |
| work_keys_str_mv | AT selenepirizpezzutto arabidopsisrootapicalmeristemadaptationtoanosmoticgradientconditionanintegratedapproachfromcellexpansiontogeneexpression AT mauromartinezmore arabidopsisrootapicalmeristemadaptationtoanosmoticgradientconditionanintegratedapproachfromcellexpansiontogeneexpression AT mariamarthasainz arabidopsisrootapicalmeristemadaptationtoanosmoticgradientconditionanintegratedapproachfromcellexpansiontogeneexpression AT omarborsani arabidopsisrootapicalmeristemadaptationtoanosmoticgradientconditionanintegratedapproachfromcellexpansiontogeneexpression AT marianasotelosilveira arabidopsisrootapicalmeristemadaptationtoanosmoticgradientconditionanintegratedapproachfromcellexpansiontogeneexpression |