Modulating gene expression as a strategy to investigate thyroid cancer biology
ABSTRACT Modulating the expression of a coding or noncoding gene is a key tool in scientific research. This strategy has evolved methodologically due to advances in cloning approaches, modeling/algorithms in short hairpin RNA (shRNA) design for knockdown efficiency, and biochemical modifications in...
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| Format: | Article |
| Language: | English |
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Brazilian Society of Endocrinology and Metabolism
2025-01-01
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| Series: | Archives of Endocrinology and Metabolism |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972024001000700&lng=en&tlng=en |
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| _version_ | 1832592461201932288 |
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| author | Diego Claro de Mello Joice Moraes Menezes Antonio Tarelo Freitas de Oliveira Marcella Maringolo Cristovão Edna Teruko Kimura Cesar Seigi Fuziwara |
| author_facet | Diego Claro de Mello Joice Moraes Menezes Antonio Tarelo Freitas de Oliveira Marcella Maringolo Cristovão Edna Teruko Kimura Cesar Seigi Fuziwara |
| author_sort | Diego Claro de Mello |
| collection | DOAJ |
| description | ABSTRACT Modulating the expression of a coding or noncoding gene is a key tool in scientific research. This strategy has evolved methodologically due to advances in cloning approaches, modeling/algorithms in short hairpin RNA (shRNA) design for knockdown efficiency, and biochemical modifications in RNA synthesis, among other developments. Overall, these modifications have improved the ways to either reduce or induce the expression of a given gene with efficiency and facility for implementation in the lab. Allied with that, the existence of various human cell line models for cancer covering different histotypes and biological behaviors, especially for thyroid cancer, has helped improve the understanding of cancer biology. In this review, we cover the most frequently used current techniques for gene modulation in the thyroid cancer field, such as RNA interference (RNAi), short hairpin RNA (shRNA), and gene editing with CRISPR/Cas9 for inhibiting a target gene, and strategies to overexpress a gene, such as plasmid cloning and CRISPRa. Exploring the possibilities for gene modulation allows the improvement of the scientific quality of the studies and the integration of clinicians and basic scientists, leading to better development of translational research. |
| format | Article |
| id | doaj-art-9632cb79a8344d349414c04322ffec9f |
| institution | Kabale University |
| issn | 2359-4292 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Brazilian Society of Endocrinology and Metabolism |
| record_format | Article |
| series | Archives of Endocrinology and Metabolism |
| spelling | doaj-art-9632cb79a8344d349414c04322ffec9f2025-01-21T07:45:10ZengBrazilian Society of Endocrinology and MetabolismArchives of Endocrinology and Metabolism2359-42922025-01-0168spe110.20945/2359-4292-2024-0073Modulating gene expression as a strategy to investigate thyroid cancer biologyDiego Claro de Mellohttps://orcid.org/0000-0002-6405-0635Joice Moraes Menezeshttps://orcid.org/0000-0001-6399-3095Antonio Tarelo Freitas de Oliveirahttps://orcid.org/0009-0000-8767-4662Marcella Maringolo Cristovãohttps://orcid.org/0000-0001-9110-5788Edna Teruko Kimurahttps://orcid.org/0000-0001-8403-4459Cesar Seigi Fuziwarahttps://orcid.org/0000-0002-0567-5432ABSTRACT Modulating the expression of a coding or noncoding gene is a key tool in scientific research. This strategy has evolved methodologically due to advances in cloning approaches, modeling/algorithms in short hairpin RNA (shRNA) design for knockdown efficiency, and biochemical modifications in RNA synthesis, among other developments. Overall, these modifications have improved the ways to either reduce or induce the expression of a given gene with efficiency and facility for implementation in the lab. Allied with that, the existence of various human cell line models for cancer covering different histotypes and biological behaviors, especially for thyroid cancer, has helped improve the understanding of cancer biology. In this review, we cover the most frequently used current techniques for gene modulation in the thyroid cancer field, such as RNA interference (RNAi), short hairpin RNA (shRNA), and gene editing with CRISPR/Cas9 for inhibiting a target gene, and strategies to overexpress a gene, such as plasmid cloning and CRISPRa. Exploring the possibilities for gene modulation allows the improvement of the scientific quality of the studies and the integration of clinicians and basic scientists, leading to better development of translational research.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972024001000700&lng=en&tlng=enRNA interferencemicroRNACRISPR/Cas9thyroid cancer |
| spellingShingle | Diego Claro de Mello Joice Moraes Menezes Antonio Tarelo Freitas de Oliveira Marcella Maringolo Cristovão Edna Teruko Kimura Cesar Seigi Fuziwara Modulating gene expression as a strategy to investigate thyroid cancer biology Archives of Endocrinology and Metabolism RNA interference microRNA CRISPR/Cas9 thyroid cancer |
| title | Modulating gene expression as a strategy to investigate thyroid cancer biology |
| title_full | Modulating gene expression as a strategy to investigate thyroid cancer biology |
| title_fullStr | Modulating gene expression as a strategy to investigate thyroid cancer biology |
| title_full_unstemmed | Modulating gene expression as a strategy to investigate thyroid cancer biology |
| title_short | Modulating gene expression as a strategy to investigate thyroid cancer biology |
| title_sort | modulating gene expression as a strategy to investigate thyroid cancer biology |
| topic | RNA interference microRNA CRISPR/Cas9 thyroid cancer |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972024001000700&lng=en&tlng=en |
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