Lineage analysis of human papillomavirus types 33 and 35 based on E6 gene in cervical samples from Tehran, Iran
Abstract Knowing the geographical distribution of lineages and sublineages of each HPV type is beneficial for the epidemiological and evolutionary studies of this virus. However, no studies have analyzed the variants of HPV 33 and 35 in Iranian women. To investigate the sequence variation of HPV 33...
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Nature Portfolio
2025-04-01
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| author | Mohammad Shabanpour Sima Taherkhani Zabihollah Shoja Mahtab Eftekhari Somayeh Jalilvand |
| author_facet | Mohammad Shabanpour Sima Taherkhani Zabihollah Shoja Mahtab Eftekhari Somayeh Jalilvand |
| author_sort | Mohammad Shabanpour |
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| description | Abstract Knowing the geographical distribution of lineages and sublineages of each HPV type is beneficial for the epidemiological and evolutionary studies of this virus. However, no studies have analyzed the variants of HPV 33 and 35 in Iranian women. To investigate the sequence variation of HPV 33 and 35 E6 gene, 42 and 92 samples were analyzed using nested PCR and sequencing, respectively. Sublineage analysis of HPV 33 showed that most of the samples belonged to the A2 sublineage (85.8%) and the remaining were classified to the A1 sublineage. Four mutations at positions A132C, A213C, A364C, and A480T were detected, among which two mutations A213C and A364C led to amino acid changes corresponding to positions K35N and N86H of amino acid, respectively, in 85.8% of samples. For HPV 35, two sublineages A1 and A2 were found in studied samples with a prevalence of 84.8 and 15.2%, respectively. Seven nucleotide changes were observed at positions of C127G/T, A130C, A131C, C136T, G249T, A326G, and C341T. Three of these mutations including A130C, G249T, and C341T resulted in amino acid changes at positions of E7D, C47F, and R78W in 4.4%, 3.3%, and 9.8% of samples, respectively. Concerning HPV 33 or 35 distinct lineages by histology/cytology status, no statistically significant differences were observed. Our results indicated that sublineages A2 of HPV 33 and A1 of HPV 35 were dominant in Tehran, Iran. However, more studies with larger sample sizes from different histopathological stages of cervix in various geographical regions of Iran are necessary to evaluate the pathogenicity risk of HPV 33 and 35 (sub)lineages in Iranian women with cervical cancer in the future. |
| format | Article |
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| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
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| spelling | doaj-art-962c2b8026fb400dac092a962d1d4bc62025-08-20T03:14:07ZengNature PortfolioScientific Reports2045-23222025-04-011511810.1038/s41598-025-97080-6Lineage analysis of human papillomavirus types 33 and 35 based on E6 gene in cervical samples from Tehran, IranMohammad Shabanpour0Sima Taherkhani1Zabihollah Shoja2Mahtab Eftekhari3Somayeh Jalilvand4Department of Virology, School of Public Health, Tehran University of Medical SciencesDepartment of Virology, School of Public Health, Tehran University of Medical SciencesDepartment of Virology, Pasteur Institute of IranDepartment of Virology, School of Public Health, Tehran University of Medical SciencesDepartment of Virology, School of Public Health, Tehran University of Medical SciencesAbstract Knowing the geographical distribution of lineages and sublineages of each HPV type is beneficial for the epidemiological and evolutionary studies of this virus. However, no studies have analyzed the variants of HPV 33 and 35 in Iranian women. To investigate the sequence variation of HPV 33 and 35 E6 gene, 42 and 92 samples were analyzed using nested PCR and sequencing, respectively. Sublineage analysis of HPV 33 showed that most of the samples belonged to the A2 sublineage (85.8%) and the remaining were classified to the A1 sublineage. Four mutations at positions A132C, A213C, A364C, and A480T were detected, among which two mutations A213C and A364C led to amino acid changes corresponding to positions K35N and N86H of amino acid, respectively, in 85.8% of samples. For HPV 35, two sublineages A1 and A2 were found in studied samples with a prevalence of 84.8 and 15.2%, respectively. Seven nucleotide changes were observed at positions of C127G/T, A130C, A131C, C136T, G249T, A326G, and C341T. Three of these mutations including A130C, G249T, and C341T resulted in amino acid changes at positions of E7D, C47F, and R78W in 4.4%, 3.3%, and 9.8% of samples, respectively. Concerning HPV 33 or 35 distinct lineages by histology/cytology status, no statistically significant differences were observed. Our results indicated that sublineages A2 of HPV 33 and A1 of HPV 35 were dominant in Tehran, Iran. However, more studies with larger sample sizes from different histopathological stages of cervix in various geographical regions of Iran are necessary to evaluate the pathogenicity risk of HPV 33 and 35 (sub)lineages in Iranian women with cervical cancer in the future.https://doi.org/10.1038/s41598-025-97080-6Human papillomavirusHPV 33HPV 35LineageSublineageAnd cervical cancer |
| spellingShingle | Mohammad Shabanpour Sima Taherkhani Zabihollah Shoja Mahtab Eftekhari Somayeh Jalilvand Lineage analysis of human papillomavirus types 33 and 35 based on E6 gene in cervical samples from Tehran, Iran Scientific Reports Human papillomavirus HPV 33 HPV 35 Lineage Sublineage And cervical cancer |
| title | Lineage analysis of human papillomavirus types 33 and 35 based on E6 gene in cervical samples from Tehran, Iran |
| title_full | Lineage analysis of human papillomavirus types 33 and 35 based on E6 gene in cervical samples from Tehran, Iran |
| title_fullStr | Lineage analysis of human papillomavirus types 33 and 35 based on E6 gene in cervical samples from Tehran, Iran |
| title_full_unstemmed | Lineage analysis of human papillomavirus types 33 and 35 based on E6 gene in cervical samples from Tehran, Iran |
| title_short | Lineage analysis of human papillomavirus types 33 and 35 based on E6 gene in cervical samples from Tehran, Iran |
| title_sort | lineage analysis of human papillomavirus types 33 and 35 based on e6 gene in cervical samples from tehran iran |
| topic | Human papillomavirus HPV 33 HPV 35 Lineage Sublineage And cervical cancer |
| url | https://doi.org/10.1038/s41598-025-97080-6 |
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