CORRELATION OF NEOPTERINE AND IFNγ LEVELS IN THE PATIENTS WITH EXACERBATION OF CHRONIC PERIODONTITIS AND INTOLERANCE OF DENTAL MATERIALS

Studies of potential biomarkers in periodontology are focused on analysis of oral liquid which revealed a number of potential salivary periodontitis biomarkers. The biomarkers should be simple to perform, with easily interpretable results providing relevant information from initial stages of disease...

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Main Author: I. Yu. Karpuk
Format: Article
Language:Russian
Published: St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists 2017-01-01
Series:Медицинская иммунология
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Online Access:https://www.mimmun.ru/mimmun/article/view/1165
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author I. Yu. Karpuk
author_facet I. Yu. Karpuk
author_sort I. Yu. Karpuk
collection DOAJ
description Studies of potential biomarkers in periodontology are focused on analysis of oral liquid which revealed a number of potential salivary periodontitis biomarkers. The biomarkers should be simple to perform, with easily interpretable results providing relevant information from initial stages of disease, to measure its activity, thus reflecting the disease evolution (e.g., from gingivitis to periodontitis). However there are some more scientific, clinical and technological tasks to develop successful clinical salivary diagnostics for periodontitis treatment. The purpose of our study was to assess interrelations of IFNγ and neopterine in oral fluid of the patients with chronic generalized periodontitis (CGP), aiming for more complete understanding of periodontal disorders and at patients with an intolerance of stomatologic materials. It is shown that the average levels of neopterine in exacerbated moderate-stage CGP patients was raised before treatment to 11.6 [8.0; 15.4] ng/ml, as compared with post-treatment level of 5,5 [4,1; 7,1] ng/ml (р < 0.05) Average amount of IFNγ in oral fluid from these patients group was 6.7 [5.0; 8.7] pg/ml before treatment, as compared to 5.5 [3.5; 7.1] pg/ml after treatment (no significant difference). Neopterine level in patients with exacerbated severe CGP before treatment was even higher than in patients of moderate/severe group, with an average of 8.2 [6.0; 9.9] ng/ml thus sufficiently exceeding post-treatment levels, i.e., 5.5 [4.1; 7.1] ng/ml (р < 0.05). A course of periodontal therapy in this group was associated with sufficient changes of IFNγ levels in oral fluid: appropriate levels before and after treatment were, respectively, 8.6 [7.3; 11.2] pg/ml, and 5.4 [4.3; 6.7] pg/ml. We have revealed that the OHI-S and SBI indexes characterizing conditions of oral cavity are interconnected with local neopterine and IFNγ levels. It is shown that an increased neopterine concentration in oral fluid and its correlation with SBI index in CGP patients is an independent biomarker of therapeutic effect. The patients with a CGP exhibited a highly significant correlation between initial levels of IFNγ and neopterine in oral fluid (R = 0.82; p = 0.0001), and after treatment (R = 0.78; p = 0.0001) in both groups of the patients.Level of a neopterin and IFNγ at patients with complaints to intolerance of dentoprosthetic materials before removal of artificial limbs and after removal of orthopedic designs authentically didn't change.
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spelling doaj-art-962ae63dab634f8088efe4d84eff3a282025-08-20T03:37:45ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2017-01-01191556410.15789/1563-0625-2017-1-55-64882CORRELATION OF NEOPTERINE AND IFNγ LEVELS IN THE PATIENTS WITH EXACERBATION OF CHRONIC PERIODONTITIS AND INTOLERANCE OF DENTAL MATERIALSI. Yu. Karpuk0Vitebsk State Medical UniversityStudies of potential biomarkers in periodontology are focused on analysis of oral liquid which revealed a number of potential salivary periodontitis biomarkers. The biomarkers should be simple to perform, with easily interpretable results providing relevant information from initial stages of disease, to measure its activity, thus reflecting the disease evolution (e.g., from gingivitis to periodontitis). However there are some more scientific, clinical and technological tasks to develop successful clinical salivary diagnostics for periodontitis treatment. The purpose of our study was to assess interrelations of IFNγ and neopterine in oral fluid of the patients with chronic generalized periodontitis (CGP), aiming for more complete understanding of periodontal disorders and at patients with an intolerance of stomatologic materials. It is shown that the average levels of neopterine in exacerbated moderate-stage CGP patients was raised before treatment to 11.6 [8.0; 15.4] ng/ml, as compared with post-treatment level of 5,5 [4,1; 7,1] ng/ml (р < 0.05) Average amount of IFNγ in oral fluid from these patients group was 6.7 [5.0; 8.7] pg/ml before treatment, as compared to 5.5 [3.5; 7.1] pg/ml after treatment (no significant difference). Neopterine level in patients with exacerbated severe CGP before treatment was even higher than in patients of moderate/severe group, with an average of 8.2 [6.0; 9.9] ng/ml thus sufficiently exceeding post-treatment levels, i.e., 5.5 [4.1; 7.1] ng/ml (р < 0.05). A course of periodontal therapy in this group was associated with sufficient changes of IFNγ levels in oral fluid: appropriate levels before and after treatment were, respectively, 8.6 [7.3; 11.2] pg/ml, and 5.4 [4.3; 6.7] pg/ml. We have revealed that the OHI-S and SBI indexes characterizing conditions of oral cavity are interconnected with local neopterine and IFNγ levels. It is shown that an increased neopterine concentration in oral fluid and its correlation with SBI index in CGP patients is an independent biomarker of therapeutic effect. The patients with a CGP exhibited a highly significant correlation between initial levels of IFNγ and neopterine in oral fluid (R = 0.82; p = 0.0001), and after treatment (R = 0.78; p = 0.0001) in both groups of the patients.Level of a neopterin and IFNγ at patients with complaints to intolerance of dentoprosthetic materials before removal of artificial limbs and after removal of orthopedic designs authentically didn't change.https://www.mimmun.ru/mimmun/article/view/1165neopterinechronic periodontitisinterferon-gammadiagnosticsbiomarker
spellingShingle I. Yu. Karpuk
CORRELATION OF NEOPTERINE AND IFNγ LEVELS IN THE PATIENTS WITH EXACERBATION OF CHRONIC PERIODONTITIS AND INTOLERANCE OF DENTAL MATERIALS
Медицинская иммунология
neopterine
chronic periodontitis
interferon-gamma
diagnostics
biomarker
title CORRELATION OF NEOPTERINE AND IFNγ LEVELS IN THE PATIENTS WITH EXACERBATION OF CHRONIC PERIODONTITIS AND INTOLERANCE OF DENTAL MATERIALS
title_full CORRELATION OF NEOPTERINE AND IFNγ LEVELS IN THE PATIENTS WITH EXACERBATION OF CHRONIC PERIODONTITIS AND INTOLERANCE OF DENTAL MATERIALS
title_fullStr CORRELATION OF NEOPTERINE AND IFNγ LEVELS IN THE PATIENTS WITH EXACERBATION OF CHRONIC PERIODONTITIS AND INTOLERANCE OF DENTAL MATERIALS
title_full_unstemmed CORRELATION OF NEOPTERINE AND IFNγ LEVELS IN THE PATIENTS WITH EXACERBATION OF CHRONIC PERIODONTITIS AND INTOLERANCE OF DENTAL MATERIALS
title_short CORRELATION OF NEOPTERINE AND IFNγ LEVELS IN THE PATIENTS WITH EXACERBATION OF CHRONIC PERIODONTITIS AND INTOLERANCE OF DENTAL MATERIALS
title_sort correlation of neopterine and ifnγ levels in the patients with exacerbation of chronic periodontitis and intolerance of dental materials
topic neopterine
chronic periodontitis
interferon-gamma
diagnostics
biomarker
url https://www.mimmun.ru/mimmun/article/view/1165
work_keys_str_mv AT iyukarpuk correlationofneopterineandifnglevelsinthepatientswithexacerbationofchronicperiodontitisandintoleranceofdentalmaterials