Comparison of Development and Antioxidative Ability in Fertilized Crossbred (Yorkshire × Landrace × Duroc) Oocytes Using Duroc and Landrace Sperm
Pig production through crossbreeding methods is a pillar of the swine industry; however, research on the fertilization ability of male pigs in crossbreeds is lacking. Therefore, this study investigated the effects of Duroc sperm (DS) and Landrace sperm (LS) on fertility in Yorkshire × Landrace × Dur...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/14/24/3562 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850041192465563648 |
|---|---|
| author | Hayoung Lee Hyewon Kim Jisoon An Hee-Tae Cheong Sang-Hee Lee |
| author_facet | Hayoung Lee Hyewon Kim Jisoon An Hee-Tae Cheong Sang-Hee Lee |
| author_sort | Hayoung Lee |
| collection | DOAJ |
| description | Pig production through crossbreeding methods is a pillar of the swine industry; however, research on the fertilization ability of male pigs in crossbreeds is lacking. Therefore, this study investigated the effects of Duroc sperm (DS) and Landrace sperm (LS) on fertility in Yorkshire × Landrace × Duroc (YLD) oocytes. Sperm were collected from the Duroc and Landrace species, and sperm characteristics, viability, and acrosome reactions were analyzed using flow cytometry. Oocytes were collected from YLD ovaries, and the fertility of DS and LS was determined using in vitro fertilization (IVF). Reactive oxygen species (ROS) and antioxidative abilities were analyzed using H<sub>2</sub>DCFDA and a Cell Tracker Red assay. Pluripotency (<i>OCT4</i>, <i>SOX2</i>, and <i>NANOG)</i>, antioxidative (<i>SOD1</i>, <i>SOD2</i>, <i>CAT</i>, and <i>GPx1</i>), apoptotic (<i>Bax</i> and <i>Bcl-2</i>), and cell cycle-related (<i>Cdc2</i> and <i>CCNB1</i>) genes were detected using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in oocytes fertilized with sperm. The results showed no significant difference in viability or acrosome reaction between DS and LS. ROS levels were significantly lower in the LS group than in the DS group, whereas glutathione (GSH) levels in the embryo did not significantly differ between the DS and LS groups. The <i>OCT4</i>, <i>GPx1</i>, and <i>Cdc2</i> mRNA expression levels were significantly higher in the LS than DS groups. Blastocyst formation was significantly higher in the LS than DS groups. ROS levels were reduced, and blastocyte formation was increased in LS-obtained embryos. In conclusion, these results provide a fundamental understanding of using Landrace semen in the three-way crossbreeding of YLD pigs. |
| format | Article |
| id | doaj-art-962a8129c11d48369e4151d84d041d4b |
| institution | DOAJ |
| issn | 2076-2615 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-962a8129c11d48369e4151d84d041d4b2025-08-20T02:55:49ZengMDPI AGAnimals2076-26152024-12-011424356210.3390/ani14243562Comparison of Development and Antioxidative Ability in Fertilized Crossbred (Yorkshire × Landrace × Duroc) Oocytes Using Duroc and Landrace SpermHayoung Lee0Hyewon Kim1Jisoon An2Hee-Tae Cheong3Sang-Hee Lee4College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Republic of KoreaCollege of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Republic of KoreaCollege of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Republic of KoreaCollege of Veterinary Sciences, Kangwon National University, Chuncheon 24341, Republic of KoreaCollege of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Republic of KoreaPig production through crossbreeding methods is a pillar of the swine industry; however, research on the fertilization ability of male pigs in crossbreeds is lacking. Therefore, this study investigated the effects of Duroc sperm (DS) and Landrace sperm (LS) on fertility in Yorkshire × Landrace × Duroc (YLD) oocytes. Sperm were collected from the Duroc and Landrace species, and sperm characteristics, viability, and acrosome reactions were analyzed using flow cytometry. Oocytes were collected from YLD ovaries, and the fertility of DS and LS was determined using in vitro fertilization (IVF). Reactive oxygen species (ROS) and antioxidative abilities were analyzed using H<sub>2</sub>DCFDA and a Cell Tracker Red assay. Pluripotency (<i>OCT4</i>, <i>SOX2</i>, and <i>NANOG)</i>, antioxidative (<i>SOD1</i>, <i>SOD2</i>, <i>CAT</i>, and <i>GPx1</i>), apoptotic (<i>Bax</i> and <i>Bcl-2</i>), and cell cycle-related (<i>Cdc2</i> and <i>CCNB1</i>) genes were detected using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in oocytes fertilized with sperm. The results showed no significant difference in viability or acrosome reaction between DS and LS. ROS levels were significantly lower in the LS group than in the DS group, whereas glutathione (GSH) levels in the embryo did not significantly differ between the DS and LS groups. The <i>OCT4</i>, <i>GPx1</i>, and <i>Cdc2</i> mRNA expression levels were significantly higher in the LS than DS groups. Blastocyst formation was significantly higher in the LS than DS groups. ROS levels were reduced, and blastocyte formation was increased in LS-obtained embryos. In conclusion, these results provide a fundamental understanding of using Landrace semen in the three-way crossbreeding of YLD pigs.https://www.mdpi.com/2076-2615/14/24/3562Durocembryo developmentLandracesperm characteristics |
| spellingShingle | Hayoung Lee Hyewon Kim Jisoon An Hee-Tae Cheong Sang-Hee Lee Comparison of Development and Antioxidative Ability in Fertilized Crossbred (Yorkshire × Landrace × Duroc) Oocytes Using Duroc and Landrace Sperm Animals Duroc embryo development Landrace sperm characteristics |
| title | Comparison of Development and Antioxidative Ability in Fertilized Crossbred (Yorkshire × Landrace × Duroc) Oocytes Using Duroc and Landrace Sperm |
| title_full | Comparison of Development and Antioxidative Ability in Fertilized Crossbred (Yorkshire × Landrace × Duroc) Oocytes Using Duroc and Landrace Sperm |
| title_fullStr | Comparison of Development and Antioxidative Ability in Fertilized Crossbred (Yorkshire × Landrace × Duroc) Oocytes Using Duroc and Landrace Sperm |
| title_full_unstemmed | Comparison of Development and Antioxidative Ability in Fertilized Crossbred (Yorkshire × Landrace × Duroc) Oocytes Using Duroc and Landrace Sperm |
| title_short | Comparison of Development and Antioxidative Ability in Fertilized Crossbred (Yorkshire × Landrace × Duroc) Oocytes Using Duroc and Landrace Sperm |
| title_sort | comparison of development and antioxidative ability in fertilized crossbred yorkshire landrace duroc oocytes using duroc and landrace sperm |
| topic | Duroc embryo development Landrace sperm characteristics |
| url | https://www.mdpi.com/2076-2615/14/24/3562 |
| work_keys_str_mv | AT hayounglee comparisonofdevelopmentandantioxidativeabilityinfertilizedcrossbredyorkshirelandracedurocoocytesusingdurocandlandracesperm AT hyewonkim comparisonofdevelopmentandantioxidativeabilityinfertilizedcrossbredyorkshirelandracedurocoocytesusingdurocandlandracesperm AT jisoonan comparisonofdevelopmentandantioxidativeabilityinfertilizedcrossbredyorkshirelandracedurocoocytesusingdurocandlandracesperm AT heetaecheong comparisonofdevelopmentandantioxidativeabilityinfertilizedcrossbredyorkshirelandracedurocoocytesusingdurocandlandracesperm AT sangheelee comparisonofdevelopmentandantioxidativeabilityinfertilizedcrossbredyorkshirelandracedurocoocytesusingdurocandlandracesperm |