Anti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene.
<h4>Background</h4>Epidemiological studies have shown that unfiltered coffee consumption is associated with a low incidence of cancer. This study aims to identify the effects of kahweol, an antioxidant diterpene contained in unfiltered coffee, on angiogenesis and key inflammatory molecul...
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0023407&type=printable |
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| author | Casimiro Cárdenas Ana R Quesada Miguel A Medina |
| author_facet | Casimiro Cárdenas Ana R Quesada Miguel A Medina |
| author_sort | Casimiro Cárdenas |
| collection | DOAJ |
| description | <h4>Background</h4>Epidemiological studies have shown that unfiltered coffee consumption is associated with a low incidence of cancer. This study aims to identify the effects of kahweol, an antioxidant diterpene contained in unfiltered coffee, on angiogenesis and key inflammatory molecules.<h4>Methodology/principal findings</h4>The experimental procedures included in vivo angiogenesis assays (both the chicken and quail choriallantoic membrane assay and the angiogenesis assay with fluorescent zebrafish), the ex vivo mouse aortic ring assay and the in vitro analysis of the effects of treatment of human endothelial cells with kahweol in cell growth, cell viability, cell migration and zymographic assays, as well as the tube formation assay on Matrigel. Additionally, two inflammation markers were determined, namely, the expression levels of cyclooxygenase 2 and the levels of secreted monocyte chemoattractant protein-1. We show for the first time that kahweol is an anti-angiogenic compound with inhibitory effects in two in vivo and one ex vivo angiogenesis models, with effects on specific steps of the angiogenic process: endothelial cell proliferation, migration, invasion and tube formation on Matrigel. We also demonstrate the inhibitory effect of kahweol on the endothelial cell potential to remodel extracellular matrix by targeting two key molecules involved in the process, MMP-2 and uPA. Finally, the anti-inflammatory potential of this compound is demonstrated by its inhibition of both COX-2 expression and MCP-1 secretion in endothelial cells.<h4>Conclusion/significance</h4>Taken together, our data indicate that, indeed, kahweol behaves as an anti-inflammatory and anti-angiogenic compound with potential use in antitumoral therapies. These data may contribute to the explanation of the reported antitumoral effects of kahweol, including the recent epidemiological meta-analysis showing that drinking coffee could decrease the risk of certain cancers. |
| format | Article |
| id | doaj-art-96259c5b9dfd470181af55a852e17b4a |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-96259c5b9dfd470181af55a852e17b4a2025-08-20T02:48:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0168e2340710.1371/journal.pone.0023407Anti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene.Casimiro CárdenasAna R QuesadaMiguel A Medina<h4>Background</h4>Epidemiological studies have shown that unfiltered coffee consumption is associated with a low incidence of cancer. This study aims to identify the effects of kahweol, an antioxidant diterpene contained in unfiltered coffee, on angiogenesis and key inflammatory molecules.<h4>Methodology/principal findings</h4>The experimental procedures included in vivo angiogenesis assays (both the chicken and quail choriallantoic membrane assay and the angiogenesis assay with fluorescent zebrafish), the ex vivo mouse aortic ring assay and the in vitro analysis of the effects of treatment of human endothelial cells with kahweol in cell growth, cell viability, cell migration and zymographic assays, as well as the tube formation assay on Matrigel. Additionally, two inflammation markers were determined, namely, the expression levels of cyclooxygenase 2 and the levels of secreted monocyte chemoattractant protein-1. We show for the first time that kahweol is an anti-angiogenic compound with inhibitory effects in two in vivo and one ex vivo angiogenesis models, with effects on specific steps of the angiogenic process: endothelial cell proliferation, migration, invasion and tube formation on Matrigel. We also demonstrate the inhibitory effect of kahweol on the endothelial cell potential to remodel extracellular matrix by targeting two key molecules involved in the process, MMP-2 and uPA. Finally, the anti-inflammatory potential of this compound is demonstrated by its inhibition of both COX-2 expression and MCP-1 secretion in endothelial cells.<h4>Conclusion/significance</h4>Taken together, our data indicate that, indeed, kahweol behaves as an anti-inflammatory and anti-angiogenic compound with potential use in antitumoral therapies. These data may contribute to the explanation of the reported antitumoral effects of kahweol, including the recent epidemiological meta-analysis showing that drinking coffee could decrease the risk of certain cancers.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0023407&type=printable |
| spellingShingle | Casimiro Cárdenas Ana R Quesada Miguel A Medina Anti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene. PLoS ONE |
| title | Anti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene. |
| title_full | Anti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene. |
| title_fullStr | Anti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene. |
| title_full_unstemmed | Anti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene. |
| title_short | Anti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene. |
| title_sort | anti angiogenic and anti inflammatory properties of kahweol a coffee diterpene |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0023407&type=printable |
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